Combining single-cell analysis and molecular docking techniques to construct a prognostic model for colon adenocarcinoma and uncovering inhibin subunit βb as a novel therapeutic target DOI Creative Commons

Qinqing Wu,

Ye Lu,

Yuwei Wu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 9, 2025

Colon adenocarcinoma (COAD) is a malignancy with high mortality rate and complex biological characteristics heterogeneity, which poses challenges for clinical treatment. Anoikis type of programmed cell death that occurs when cells lose their attachment to the extracellular matrix (ECM), it plays crucial role in tumor metastasis. However, specific link between anoikis COAD, as well its mechanisms progression, remains unclear, making potential new direction therapeutic strategy research. We employed transcriptomic data information from The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) pinpoint differentially expressed anoikis-related genes (ARGs) COAD. Using Cox proportional hazards models Lasso regression analysis, we developed prognostic signature derived these ARGs. also investigated roles interactions microenvironment by analyzing single-cell RNA sequencing data. Additionally, molecular docking techniques evaluate inhibin subunit beta B (INHBB) targets assess binding affinity candidate drugs. Finally, used gene knockout silence key INHBB explored functions vitro. In our study, expression differences ARGs, successfully classified patients Kaplan-Meier survival analysis demonstrated elevated risk scores experienced poorer prognosis, finding was confirmed both training validation cohorts. immune infiltration revealed notable increase presence within high-risk patients. Molecular identified drug candidates INHBB, including risperidone. Furthermore, vitro experiments showed downregulation COAD lines significantly reduced cellular viability migration capacity. summary, research, based on provides insights into precise classification, prognosis assessment, identification It validates progression establishing foundation future personalized treatment strategies.

Язык: Английский

Chitosan-based hydrogels in cancer therapy: Drug and gene delivery, stimuli-responsive carriers, phototherapy and immunotherapy DOI

Hailin Zhu,

Haowei Sun, Jingyuan Dai

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 282, С. 137047 - 137047

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

4
Combining single-cell analysis and molecular docking techniques to construct a prognostic model for colon adenocarcinoma and uncovering inhibin subunit βb as a novel therapeutic target DOI Creative Commons

Qinqing Wu,

Ye Lu,

Yuwei Wu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 9, 2025

Colon adenocarcinoma (COAD) is a malignancy with high mortality rate and complex biological characteristics heterogeneity, which poses challenges for clinical treatment. Anoikis type of programmed cell death that occurs when cells lose their attachment to the extracellular matrix (ECM), it plays crucial role in tumor metastasis. However, specific link between anoikis COAD, as well its mechanisms progression, remains unclear, making potential new direction therapeutic strategy research. We employed transcriptomic data information from The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) pinpoint differentially expressed anoikis-related genes (ARGs) COAD. Using Cox proportional hazards models Lasso regression analysis, we developed prognostic signature derived these ARGs. also investigated roles interactions microenvironment by analyzing single-cell RNA sequencing data. Additionally, molecular docking techniques evaluate inhibin subunit beta B (INHBB) targets assess binding affinity candidate drugs. Finally, used gene knockout silence key INHBB explored functions vitro. In our study, expression differences ARGs, successfully classified patients Kaplan-Meier survival analysis demonstrated elevated risk scores experienced poorer prognosis, finding was confirmed both training validation cohorts. immune infiltration revealed notable increase presence within high-risk patients. Molecular identified drug candidates INHBB, including risperidone. Furthermore, vitro experiments showed downregulation COAD lines significantly reduced cellular viability migration capacity. summary, research, based on provides insights into precise classification, prognosis assessment, identification It validates progression establishing foundation future personalized treatment strategies.

Язык: Английский

Процитировано

0