Application of Invasive Bacteria for the Delivery of Anti-Cancer Therapeutics DOI Open Access
Rasaq Akinsola, Kumaran Narayanan

Therapeutics, Год журнала: 2024, Номер 1(2), С. 124 - 141

Опубликована: Дек. 20, 2024

Bacterial vectors for biomolecule delivery to targeted organelles, facilitating temporary or continuous protein production, have emerged as a promising approach treating acquired and inherited diseases. This method offers selective cancer eradication targeting strategy with minimal side effects. provide an alternative viral gene delivery, given their capacity deliver large genetic materials while inducing immunogenicity cytotoxicity. Bacteria such Bifidobacterium, Salmonella, Clostridium, Streptococcus demonstrated potential tumor-targeted serve oncolytic bacteria. These also been used transfer amplify genes encoding biomolecules pro-drug-converting enzymes, toxins, angiogenesis inhibitors, cytokines. The microenvironment of necrotic tumors unique opportunity therapy the non-pathogenic anaerobic bacterium. For example, Clostridium sporogenes can germinate selectively in regions upon injection endospores, which helps enhance specificity sporogenes, resulting tumor-specific colonization. Also, E. coli Salmonella sp. be capacitated hypoxic sensing promotor into core region solid tumors. uniqueness tumor microenvironment, including hypoxia, immunosuppression, metabolite deficiency enrichment, necrosis, enables bacteria tumor. Combining traditional bacterial will significantly complement cover limitations other treatments. review provides overview use vector therapy, discussing strategies maximize efficiency address challenges. In this review, we discuss anti-cancer therapeutics focusing on therapeutic strategies. We highlight complementary therapies mechanism immunotherapy perspectives future use.

Язык: Английский

Targeted delivery and controlled release of polymeric nanomedicines for tumor therapy DOI Creative Commons
Na Shen, Weidong Zhao,

Hongyu Chu

и другие.

Fundamental Research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1

Bacterial Systems as a Precision Delivery Platform of Therapeutic Peptides for Cancer Therapy DOI Creative Commons
Jinyuan Liu, Guizhi Shen, Xuehai Yan

и другие.

Polymer science & technology., Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Язык: Английский

Процитировано

1

Advances in Engineered Bacteria for Cancer Therapy DOI Creative Commons
Wenping Pan, Hongmei Liu, Decheng Wu

и другие.

Precision medicine and engineering., Год журнала: 2025, Номер unknown, С. 100017 - 100017

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Thermal-responsive ultrasound activatable in situ production of therapeutics for real-time imaging and targeted tumor therapy DOI Creative Commons

Meng Du,

Fengyi Zeng, Ting Wang

и другие.

Theranostics, Год журнала: 2025, Номер 15(9), С. 4212 - 4228

Опубликована: Март 18, 2025

Rationale: Engineered bacteria with tumor-targeting capabilities and genetic modification potential have emerged as promising vectors for targeted tumor therapy through the effective production release of therapeutic molecules. Controlled gene expression real-time monitoring protein in tumors are great significance optimizing engineered well anti-tumor effects. Methods: We constructed E. coli MG1655 cells using recombinant thermal plasmid pBV220 carrying optical imaging reporter miRFP720 Cytolysin A, which could be controlled expressed synchronously by effect produced focused ultrasound. The concentrations A after ultrasound irradiation were detected vitro vivo western blot analysis enzyme-linked immunosorbent assay (ELISA) analysis. immune cell infiltration rate inflammation factors treatment flow cytometry ELISA efficacy alone or combined anti-Programmed death 1 ligand (aPD-L1) immunotherapy evaluated on subcutaneous models orthotopic glioblastoma models. Results: Upon ultrasound-controlled activation, level molecule probe enhanced. Importantly, monitored non-invasively signal intensity miRFP720, provided guidance optimized ultrasound-mediated situ. Furthermore, not only exerted potent effects but also induced immunogenic death, enhancing efficiency aPD-L1 deep site treatment. Conclusion: In this study, a guided activatable mode was established. Utilizing ultrasound, A-miRFP720 can concurrently express mode, realized enhanced local immunotherapy.

Язык: Английский

Процитировано

0

Living Therapeutics: Precision Diagnosis and Therapy with Engineered Bacteria DOI

Ruyan Xie,

Duoyang Fan, Cheng Xiang

и другие.

Biomaterials, Год журнала: 2025, Номер 321, С. 123342 - 123342

Опубликована: Апрель 15, 2025

Язык: Английский

Процитировано

0

Engineered Bacteria: Strategies and Applications in Cancer Immunotherapy DOI Creative Commons
Shuhao Zhang, Rui Li, Yunxue Xu

и другие.

Fundamental Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

2

Bacterial Outer Membrane Vesicle (OMV)-Encapsulated TiO2 Nanoparticles: A Dual-Action Strategy for Enhanced Radiotherapy and Immunomodulation in Oral Cancer Treatment DOI Creative Commons
Shun-Li Kan, Liwen Zhang, Yu‐Chi Wang

и другие.

Nanomaterials, Год журнала: 2024, Номер 14(24), С. 2045 - 2045

Опубликована: Дек. 20, 2024

Oral squamous-cell carcinoma (OSCC) poses significant treatment challenges due to its high recurrence rates and the limitations of current therapies. Titanium dioxide (TiO2) nanoparticles are promising radiosensitizers, while bacterial outer membrane vesicles (OMVs) known for their immunomodulatory properties. This study investigates potential OMV-encapsulated TiO2 (TiO2@OMV) combine these effects improved OSCC treatment. were synthesized using a hydrothermal method encapsulated within OMVs derived from Escherichia coli. The TiO2@OMV carriers evaluated ability enhance radiosensitivity stimulate immune responses in cell lines. Reactive oxygen species (ROS) production, macrophage recruitment, selective cytotoxicity toward cancer cells assessed. demonstrated radiosensitization activation compared unencapsulated nanoparticles. system selectively induced cells, sparing normal enhanced ROS generation macrophage-mediated antitumor responses. highlights as dual-action therapeutic platform that synergizes radiotherapy immunomodulation, offering targeted effective strategy approach could improve outcomes reduce adverse associated with conventional

Язык: Английский

Процитировано

1

Application of Invasive Bacteria for the Delivery of Anti-Cancer Therapeutics DOI Open Access
Rasaq Akinsola, Kumaran Narayanan

Therapeutics, Год журнала: 2024, Номер 1(2), С. 124 - 141

Опубликована: Дек. 20, 2024

Bacterial vectors for biomolecule delivery to targeted organelles, facilitating temporary or continuous protein production, have emerged as a promising approach treating acquired and inherited diseases. This method offers selective cancer eradication targeting strategy with minimal side effects. provide an alternative viral gene delivery, given their capacity deliver large genetic materials while inducing immunogenicity cytotoxicity. Bacteria such Bifidobacterium, Salmonella, Clostridium, Streptococcus demonstrated potential tumor-targeted serve oncolytic bacteria. These also been used transfer amplify genes encoding biomolecules pro-drug-converting enzymes, toxins, angiogenesis inhibitors, cytokines. The microenvironment of necrotic tumors unique opportunity therapy the non-pathogenic anaerobic bacterium. For example, Clostridium sporogenes can germinate selectively in regions upon injection endospores, which helps enhance specificity sporogenes, resulting tumor-specific colonization. Also, E. coli Salmonella sp. be capacitated hypoxic sensing promotor into core region solid tumors. uniqueness tumor microenvironment, including hypoxia, immunosuppression, metabolite deficiency enrichment, necrosis, enables bacteria tumor. Combining traditional bacterial will significantly complement cover limitations other treatments. review provides overview use vector therapy, discussing strategies maximize efficiency address challenges. In this review, we discuss anti-cancer therapeutics focusing on therapeutic strategies. We highlight complementary therapies mechanism immunotherapy perspectives future use.

Язык: Английский

Процитировано

1