Bacterial Extracellular Vesicles and Antimicrobial Peptides: A Synergistic Approach to Overcome Antimicrobial Resistance DOI Creative Commons
Corina Ciobănaşu

Antibiotics, Год журнала: 2025, Номер 14(4), С. 414 - 414

Опубликована: Апрель 19, 2025

Antimicrobial resistance is already a major global health threat, contributing to nearly 5 million deaths annually. The rise of multidrug-resistant pathogens has made many infections increasingly difficult treat. This growing threat driven the search for alternative therapeutic approaches. Among most promising candidates are bacterial extracellular vesicles (BEVs) and antimicrobial peptides (AMPs), which offer unique mechanisms action, potential synergistic effects, ability bypass conventional pathways. review summarizes current research on effects BEVs AMPs overcome resistance.

Язык: Английский

A Simplified Method for the Isolation of Extracellular Vesicles from Probiotic Bacteria and Their Characterization DOI Open Access
Harshal Sawant, Ji Bihl, Alip Borthakur

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1058 - 1058

Опубликована: Янв. 26, 2025

Probiotic bacteria are normal inhabitants of a healthy human gut, conferring multiple beneficial effects on the gut and beyond. Under various disease states, abundance diversity significantly decreased, process called dysbiosis. Among intra- extracellular components probiotics, vesicles (EVs) secreted by them have recently garnered significant attention as potential mediators probiotics’ host health. Further, these nanosized particles that encapsulate wide range bioactive molecules (proteins, lipids, RNA, DNA) standing out key factors could mediate microbiota–host communication confer ameliorating in experimental inflammatory, metabolic, cardiovascular models. However, standard protocol EV isolation from probiotic bacteria, not varying lab to lab, must be established achieve consistency results pre-clinical Our current study compared two commonly used methods for biological samples, ultracentrifugation precipitation, develop isolating EVs probiotics Lactobacillus acidophilus (LA), Gram-positive bacterium, Escherichia coli Nissle (EcN), Gram-negative bacterium. The method gave ~1.5-fold higher yield both LA EcN precipitation method. released level LA. were quantified characterized nanoparticle-tracking analysis (NTA) measuring specific surface biomarkers using Western blot. Here, we describe our standardized step-by-step their characterization.

Язык: Английский

Процитировано

0
Mobiluncus mulieris Alters the Transcriptomic Profile of Cervicovaginal Epithelial Cells, Shedding Light on Molecular Drivers of Adverse Reproductive Outcomes DOI Creative Commons
Yu Hasegawa,

Olivia Swain,

Urvija Rajpal

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Abstract The vaginal microbiota plays a crucial role in reproductive health, with alterations its composition linked to various adverse outcomes. These outcomes are associated characterized by the depletion of Lactobacillus spp. and overgrowth anaerobic bacteria, commonly referred as community state type IV (CST IV). CST has been repeatedly bacterial vaginosis is risk factor for sexually transmitted diseases other disorders, such preterm birth (PTB). While Gardnerella frequently implicated IV, another key member, Mobiluncus mulieris, also several However, molecular mechanisms underlying contribution remain poorly understood. Using an unbiased discovery approach, this study investigated complex host-microbe interactions driven M. mulieris across distinct epithelial barriers lower tract. RNA sequencing revealed that live bacteria-free supernatant, extracellular vesicles (bEVs) induce unique transcriptional changes different cells lining Notably, all three components triggered diverse immune responses inflammatory pathways, bEVs exerting most profound effects activating inflammation through both toll-like receptor (TLR) 2 TLR5. Additionally, altered expression pathways involved matrix (ECM) organization, including increased metalloproteinase 9 (MMP9), mediator ECM degradation disorders PTB. findings were further supported clinical data showing elevated MMP9 levels pregnant women containing mulieris. results demonstrate broad impact tract epithelia how specific anaerobes drive may contribute This identifies potential therapeutic targets mitigating mulieris-driven health.

Язык: Английский

Процитировано

0
The potential use of bacteria and their derivatives as delivery systems for nanoparticles in the treatment of cancer DOI
Shiva Ahmadishoar, Saba Saeed,

Morug Salih Mahdi

и другие.

Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 54

Опубликована: Апрель 5, 2025

Cancer is a leading cause of mortality and morbidity worldwide. Nanomaterials, unique optical, magnetic, electrical properties at the nanoscale (1-100 nm), have been engineered to improve drug capacity, bioavailability, specificity in cancer treatment. These advancements address toxicity lack selectivity conventional therapies, enabling precise targeting cells, tumor microenvironment, immune system. Among emerging approaches, bacterial treatment shows promise due its natural ability target diverse therapeutic mechanisms, which nanotechnology can further enhance. Bacteria-based delivery systems leverage bacteria's adaptability survival strategies within human body. Bacterial derivatives, such as ghosts (BGs), extracellular vesicles (BEVs), dietary toxins, are recognized effective biological nanomaterials capable carrying nanoparticles (NPs). attracted increasing attention for their potential targeted NP This study explores use various bacteria byproducts vehicles, highlighting treating different types cancer. By combining strengths therapy, these innovative approaches aim revolutionize with improved precision efficacy.

Язык: Английский

Процитировано

0
Probiotic Lactobacillus‐Derived Extracellular Vesicles: Insights Into Disease Prevention and Management DOI
Pooja Yadav, Nabendu Debnath, Diwas Pradhan

и другие.

Molecular Nutrition & Food Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

ABSTRACT Bacterial extracellular vesicles (BEVs) have emerged as versatile and promising tools for therapeutic interventions across a spectrum of medical applications. Among these, Lactobacillus ‐derived (LDEVs) garnered significant attention due to their diverse physiological functions applications in health advancement. These LDEVs modulate host cell signaling pathways through the delivery bioactive molecules, including nucleic acids proteins. The immunomodulatory properties are important, they been shown regulate balance between pro‐inflammatory anti‐inflammatory responses various diseases. play crucial role maintaining gut homeostasis by modulating composition function microbiota, which has implications conditions, inflammatory bowel diseases, metabolic disorders, neurological disorders. Furthermore, hold potential deliver payloads specific tissues or organs. Engineered can be loaded with agents such antimicrobial peptides acid‐based therapies treat By leveraging unique LDEVs, researchers develop innovative strategies disease prevention, treatment, overall well‐being. Thus, this review aims provide comprehensive overview benefits promoting

Язык: Английский

Процитировано

0
Bacterial Extracellular Vesicles and Antimicrobial Peptides: A Synergistic Approach to Overcome Antimicrobial Resistance DOI Creative Commons
Corina Ciobănaşu

Antibiotics, Год журнала: 2025, Номер 14(4), С. 414 - 414

Опубликована: Апрель 19, 2025

Antimicrobial resistance is already a major global health threat, contributing to nearly 5 million deaths annually. The rise of multidrug-resistant pathogens has made many infections increasingly difficult treat. This growing threat driven the search for alternative therapeutic approaches. Among most promising candidates are bacterial extracellular vesicles (BEVs) and antimicrobial peptides (AMPs), which offer unique mechanisms action, potential synergistic effects, ability bypass conventional pathways. review summarizes current research on effects BEVs AMPs overcome resistance.

Язык: Английский

Процитировано

0