Medicine,
Год журнала:
2025,
Номер
104(12), С. e41937 - e41937
Опубликована: Март 21, 2025
Fanconi
anemia
(FA)
is
an
inherited
disorder
characterized
by
congenital
malformations,
bone
marrow
failure,
and
malignancies.
Hematopoietic
cell
transplant
(HCT)
the
only
proven
cure
for
hematological
complications.
FA
patients
have
increased
chromosomal
instability
aberrant
deoxyribonucleic
acid
repair
thus
can
tolerate
low
doses
of
chemotherapy
or
radiation
as
part
conditioning
prior
to
HCT.
Yet,
they
are
still
prone
severe
regimen
related
toxicities
including
hemorrhagic
cystitis
(HC)
from
cyclophosphamide
(CY).
As
CYP2B6
a
primary
enzyme
responsible
catalyzation
prodrug
form
CY,
understanding
association
between
genetic
variants
HC
in
may
predict
which
will
be
more
susceptible
developing
HC.
A
descriptive
study
was
performed
identify
associated
with
who
underwent
HCT
1999
2017.
All
received
CY-based
preparative
had
pretransplant
recipient
available
genomic
analysis.
Forty
pediatric
were
eligible
this
They
matched
sibling
donors
(n
=
6)
alternative
34)
failure
38)
myelodysplastic
syndrome
2).
The
incidence
32.5%
occurred
at
median
32
days
(range
20-180)
after
9
concomitant
viral
infection
(BK
virus,
n
8
both
adenovirus
BK
1).
No
significantly
top
rs2279343
(g.23060A
>
G),
rs2279344
(g.23280G
A)
gene.
among
variant
42%
(CI
22%-62%)
compared
20%
0%-40%)
those
without
(P
.19).
40%
22%-58%)
10%
0%-28%)
.11).
our
analysis
statistically
data
suggest
that
increase
risk
CY
based
therapy
but
these
must
further
evaluated
larger
population.
Journal of Cellular and Molecular Medicine,
Год журнала:
2019,
Номер
23(5), С. 3130 - 3139
Опубликована: Март 15, 2019
The
present
research
focuses
on
the
influence
of
CCCTC-binding
factor
(CTCF)
prostate
cancer
(PC)
via
regulation
FoxO
signalling
pathway.
A
bioinformatics
analysis
was
conducted
to
screen
out
target
genes
for
CTCF
in
LNCaP
cells
and
enrich
relevant
pathways
cells.
It
found
that
pathway
enriched
according
ChIP-seq
results
CTCF.
expression
CTCF,
pFoxO1a,
FoxO1a,
pFoxO3a
FoxO3a
tested
by
RT-qPCR
Western
blot.
Inhibition
could
lead
up-regulation
rates
cell
proliferation,
invasion
apoptosis
were
examined
MTT
assay,
assay
flow
cytometry
under
different
interference
conditions.
Down-regulation
suppress
facilitate
apoptosis.
Lastly,
effect
tumour
growth
determined
nude
mice.
regulated
pathway,
which
retarded
vivo.
In
conclusion,
regulates
affect
progress
PC.
Cancers,
Год журнала:
2018,
Номер
10(1), С. 25 - 25
Опубликована: Янв. 22, 2018
In
radiation
biophysics,
it
is
a
subject
of
nowadays
research
to
investigate
DNA
strand
break
repair
in
detail
after
damage
induction
by
ionizing
radiation.
It
debate
as
what
makes
up
the
cell’s
decision
use
certain
pathway
and
how
machinery
recruited
foci
spatially
temporarily
organized.
Single-molecule
localization
microscopy
(SMLM)
allows
super-resolution
analysis
precise
single
fluorescent
molecule
tags,
resulting
nuclear
structure
with
spatial
resolution
10
nm
regime.
Here,
we
used
SMLM
study
MRE11
foci.
one
three
proteins
involved
MRN-complex
(MRE11-RAD50-NBS1
complex),
prominent
resection
broken
end
bridging
component
homologous
recombination
(HRR)
alternative
non-homologous
joining
(a-NHEJ).
We
analyzed
arrangements
antibody-labelled
nuclei
breast
cancer
skin
fibroblast
cell
line
along
time-course
(up
48
h)
irradiation
dose
2
Gy.
Different
kinetics
for
cluster
formation
relaxation
were
determined.
Changes
internal
nano-scaled
clusters
quantified
compared
between
two
types.
The
results
indicate
type-dependent
response
concerning
recruitment
formation.
data
H2AX
phosphorylation
detected
γH2AX
distribution.
These
suggested
modulations
signal
frequencies
that
not
directly
correlated
induction.
application
biophysics
offers
new
possibilities
organization
damaging
during
subsequent
repair.
RNA Biology,
Год журнала:
2019,
Номер
16(9), С. 1133 - 1146
Опубликована: Июнь 18, 2019
Heterochromatic
regions
of
the
genome
are
epigenetically
regulated
to
maintain
a
heritable
'"silent
state"'.
In
fission
yeast
and
other
organisms,
epigenetic
silencing
is
guided
by
nascent
transcripts,
which
targeted
RNA
interference
pathway.
The
key
effector
complex
pathway
consists
small
interfering
molecules
(siRNAs)
associated
with
Argonaute,
assembled
into
RNA-induced
transcriptional
(RITS)
complex.
This
review
focuses
on
our
current
understanding
how
RITS
promotes
heterochromatin
formation,
in
particular
role
Argonaute-containing
complexes
many
functions
such
as
quelling,
release
polymerases,
cellular
quiescence
defense.
