The
increasing
prevalence
of
allergies
and
asthma
has
led
to
a
growing
global
socioeconomic
burden.
Since
the
outbreak
COVID-19
pandemic,
health
lifestyles
children
adolescents
have
changed
dramatically.
It's
unclear
how
this
shift
impacted
allergy
asthma,
with
limited
studies
addressing
question.
We
aim
explore
difference
among
US
during
before
pandemic
using
nationally
representative
sample
adolescents.
This
cross-sectional
study
included
31,503
participants
in
National
Health
Interview
Survey
(NHIS)
between
2018
2021.
Allergies
were
defined
on
an
affirmative
response
questionnaire
by
parent
or
guardian.
Chi-square
tests
used
compare
baseline
characteristics
for
categorical
variables.
Differences
estimated
weighted
logistic
regression,
adjusting
demographic
factors.
Interaction
analyses
explored
variations
across
strata.
In
aged
0
17,
any
was
26.1%
(95%
CI,
24.8%-
27.4%)
27.1%
25.9%-
28.2%)
Thereinto,
2018,
respiratory
allergies,
food
skin
14.0%
13.1%-
15.0%),
6.5%
5.8%-
7.1%)
12.6%
11.6%-
13.5%),
respectively,
2021,
18.8%
17.8%-
19.9%),
5.8%
5.2%-
6.4%)
10.7%
9.9%-
11.5%),
respectively.
And
11.1%
10.5%-
11.7%)
2018–2019
9.8%
9.2%-
10.4%)
2020–2021.
Prevalence
had
statistically
significant
differences.
differences
persisted
after
increased
both
decreased
compared
pre-COVID-19
pandemic.
Further
research
is
required
association
allergic
diseases
particular
emphasis
impact
lifestyle
changes
resulting
from
measures
prevent
infection.
Molecular
oxygen
(O2)
is
essential
for
numerous
metabolic
processes.
Not
surprisingly,
hypoxia
and
the
resulting
adaptations
play
a
pivotal
role
in
pathophysiology,
e.g.,
cancer
or
inflammatory
diseases.
Of
note,
myeloid
cells
are
known
to
accumulate
hypoxic
regions
such
as
tumor
cores
rheumatoid
arthritis
joints
may
contribute
disease
progression.
While
most
studies
so
far
concentrated
on
transcriptional
adaptation
by
hypoxia-inducible
factors
(HIF)
1
2
under
short
term
hypoxia,
prolonged
deprivation
alternative
post-transcriptional
regulation
rather
poorly
investigated.
Consequently,
aim
of
study
was
generate
comprehensive
overview
mRNA
de
novo
synthesis
degradation
its
contribution
total
changes
monocytic
course
hypoxia.
To
this
end,
I
used
thiol-linked
alkylation
sequencing
RNA
(SLAM-Seq)
characterize
dynamics
Specifically,
labeled
THP-1
normoxia
(N),
acute
(AH;
8
h
1%
O2),
chronic
(CH;
72
O2)
with
4-thiouridine
(4sU),
which
allows
transcriptome-wide
identification
synthesized
mRNAs
estimation
their
half-lives.
Total
expression
analyses
revealed
that
occurred
CH.
Considering
HIF
accumulation
shown
decline
again
CH,
further
analyzed
impact
stability
gene
expression.
observed
global
reduction
half-lives
indicative
attenuation
energy-consuming
protein
upon
deprivation.
Moreover,
subgroup
destabilized
transcripts
decreased
consisted
59
nuclear-encoded
mitochondrial
mRNAs.
This
might
prevent
futile
production
new
mitochondria
conditions,
where
even
actively
degraded
detrimental
reactive
species.
stability-regulated
were
mainly
vast
majority
differentially
upregulated.
Functional
not
only
but
also
cholesterol
homeostasis
response
top
enriched
terms,
corroborating
findings
level.
Focusing
hypoxia-altered
metabolism,
an
9
early
decrease
late
precursors,
separated
several
oxygen-dependent
enzymatic
steps.
Although
levels
slightly
reduced,
my
data
indicate
locally
lowered
endoplasmic
reticulum
(ER)
cause
feedback
activation
ER
cholesterol-sensing
transcription
factor
sterol
regulatory
element-binding
(SREBP2)
induction
biosynthesis
enzymes.
Interestingly,
broad
range
interferon-stimulated
genes
(ISGs),
antiviral
function,
induced
similar
kinetics
SREBP2
targets,
suggesting
immunometabolic
crosstalk.
availability
certain
intermediates
well
direct
involvement
seemed
unlikely
ISG
induction,
intracellular
distribution
appeared
crucial
chemokine-ISGs.
Mechanistically,
found
MyD88-dependent
toll-like
receptor
4
(TLR4)
signaling
contributes
enhanced
likely
sensitized
dynamics.
Importantly,
amplified
chemokine-ISGs
monocytes
treatment
severe
respiratory
syndrome
coronavirus
type
(SARS-CoV-2)
spike
via
TLR4
similarly
after
addition
infectious
virus,
systemic
inflammation
hypoxemic
patients
disease-2019
(COVID-19).
Taken
together,
comprehensively
monocytes.
identified
modulating
mechanism
limit
oxygen-restricted
conditions.
characterized
crosstalk
between
disturbed
spontaneous
interferon
(IFN)-signaling
monocytes,
cases
COVID-19.
