Loss of Sirtuin 7 impairs cell motility and proliferation and enhances S-phase cell arrest after 5-fluorouracil treatment in head and neck cancer

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 16, 2025

Sirtuin 7 (SIRT7), a member of the sirtuin family NAD+-dependent deacetylases, plays vital role in cancer, exhibiting context-dependent functions across various malignancies. Our study investigates SIRT7 depletion head and neck squamous cell carcinoma (HNSCC) progression. In vitro 3D organotypic models demonstrated that knock-out attenuates cancer viability, proliferation, motility as well induces downregulation migration- epithelial-mesenchymal transition (EMT)-related gene expression. Moreover, loss results slower organoid formation less invasive morphology, validated by vimentin downregulation. The potentiates S-phase arrest cycle progression after 5-FU treatment elevates ratio dead cells. Additionally, deletion reduces expression G1 phase-associated proteins, Cyclin D CDK4. Altogether, our highlights promising therapeutic target HNSCC, enhancing effectiveness modalities such combinational treatment.

Язык: Английский

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Xanthohumol attenuates TXNIP-mediated renal tubular injury in vitro and in vivo diabetic models

Journal of Natural Medicines, Год журнала: 2025, Номер 79(2), С. 314 - 327

Опубликована: Янв. 3, 2025

Язык: Английский

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Advances in the Regulation of Growth by SIRT1

Medical Diagnosis, Год журнала: 2025, Номер 15(01), С. 22 - 27

Опубликована: Янв. 1, 2025

Язык: Английский

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Activation of SIRT1 Reduces Renal Tubular Epithelial Cells Fibrosis in Hypoxia Through SIRT1‐FoxO1‐FoxO3‐Autophagy Pathway

Advanced Biology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Abstract Heart failure‐induced renal tubular epithelial cell fibrosis is an important pathological process that leads to chronic kidney disease. This study investigate the regulatory mechanism of over‐expression or knock‐down SIRT1 gene, alleviating hypoxia‐induced HK2 in heart failure. The focus on SIRT1‐FoxO1‐FoxO3‐Autophagy pathway. In vitro experiments are performed by HK2cell line simulate normal oxygen state (Normoxia) and hypoxia (Hypoxia) caused failure, gene transfected vectors, Rapamycin (RAPA)‐induced cellular autophagy, models divided into four subgroups, named control group, oeSIRT1, siSIRT1 siSIRT1+RAPA. Western blotting (WB), real‐time qPCR, immunofluorescence (IF), ELISA, transmission electron microscopy used quantitatively semi‐quantitatively analyze expression FoxO1, FoxO3, SIRT1, Beclin1, LC‐3, α‐SMA, E‐ Cadherin, collagen‐I cells supernatants. It demonstrated activation regulates activity FoxO1 thereby affecting autophagy. modulation a reduction markers (α‐SMA E‐cadherin) extracellular matrix deposition (collagen I), which ultimately attenuates fibrosis. These findings provide new insights potential therapeutic strategies for treating targeting

Язык: Английский

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Macrophage polarization regulation shed lights on immunotherapy for CaOx kidney stone disease

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 179, С. 117336 - 117336

Опубликована: Авг. 24, 2024

Kidney stone disease (KSD) is a major public health concern associated with high morbidity and recurrence, places significant burden on the care system worldwide. Calcium oxalate (CaOx) alone or mixture of CaOx calcium phosphate stones accounting for more than 80 % cases. However, beyond surgical removal, prevention reduction recurrence kidney have always been challenge. Given that macrophages are traditional innate immune cells play critical roles in clearance pathogens maintenance tissue homeostasis, which gained interests nephrolithiasis. Several studies recently clearly demonstrated M2-macrophage could reduce renal crystal acumination, provide premise insights therapeutic options KSD by modulating macrophage phenotypes. mechanism macrophage-polarization regulation effects treatments far from clear. Here, we comprehensively reviewed literatures related to cytokines, epigenetic modifications metabolic reprograming disease, aimed better understandings polarization as well its potential clinical applications prevention.

Язык: Английский

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Renal Fibrosis: SIRT1 Still of Value

Biomedicines, Год журнала: 2024, Номер 12(9), С. 1942 - 1942

Опубликована: Авг. 23, 2024

Chronic kidney disease (CKD) is a major global health concern. Renal fibrosis, prevalent outcome regardless of the initial cause, ultimately leads to end-stage renal disease. Glomerulosclerosis and interstitial fibrosis are primary pathological features. Preventing slowing considered effective strategies for delaying CKD progression. However, treatments lacking. Sirtuin 1 (SIRT1), nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase belonging class III histone deacetylases, implicated in physiological regulation protection susceptible diverse array influences, as demonstrated previous studies. Interestingly, controversial conclusions have emerged research has progressed. This review provides comprehensive summary current understanding advancements field; specifically, biological roles mechanisms SIRT1 regulating These include aspects such lipid metabolism, epithelial-mesenchymal transition, oxidative stress, aging, inflammation, autophagy. manuscript explores potential therapeutic target offers new perspectives on treatment approaches prognostic assessments.

Язык: Английский

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Podocyte Death in Diabetic Kidney Disease: Potential Molecular Mechanisms and Therapeutic Targets

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(16), С. 9035 - 9035

Опубликована: Авг. 20, 2024

Cell deaths maintain the normal function of tissues and organs. In pathological conditions, abnormal activation or disruption cell death often leads to pathophysiological effects. Diabetic kidney disease (DKD), a significant microvascular complication diabetes, is linked high mortality morbidity rates, imposing substantial burden on global healthcare systems economies. Loss detachment podocytes are key changes in progression DKD. This review explores potential mechanisms apoptosis, necrosis, autophagy, pyroptosis, ferroptosis, cuproptosis, podoptosis podocytes, focusing how different modes contribute It recognizes limitations current research presents latest basic clinical studies targeting podocyte pathways Lastly, it focuses future treat DKD, with intention inspiring further development therapeutic strategies.

Язык: Английский

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Apelin is Peptide Increasing Tolerance of Organs and Cells to Hypoxia and Reoxygenation. The Signaling Mechanism

International Journal of Peptide Research and Therapeutics, Год журнала: 2024, Номер 30(2)

Опубликована: Март 14, 2024

Язык: Английский

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Loss of Sirtuin 7 impairs cell motility and proliferation and enhances S-phase cell arrest after 5-fluorouracil treatment in head and neck cancer

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 14, 2024

Sirtuin 7 (SIRT7), a member of the sirtuin family NAD+-dependent deacetylases, plays vital role in cancer, exhibiting context-dependent functions across various malignancies. Our study investigates SIRT7 depletion head and neck squamous cell carcinoma (HNSCC) progression. In vitro 3D organotypic models demonstrated that knock-out attenuates cancer viability, proliferation, motility as well induces downregulation migration- epithelial-mesenchymal transition (EMT)-related gene expression. Moreover, loss potentiates slower organoid formation less invasive morphology, validated by vimentin downregulation. The S-phase arrest cycle progression after 5-FU treatment elevates ratio dead cells. Additionally, deletion reduces expression G1 phase-associated proteins, Cyclin D CDK4. Altogether, our highlights promising therapeutic target HNSCC, enhancing effectiveness modalities such combinational treatment.

Язык: Английский

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Gut microbiota-derived TMAO and SIRT1/HMGB1 Axis: unveiling mechanisms of renal impairment in beta-thalassemia major

Pediatric Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Язык: Английский

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