Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 20, 2025

Spinal cord injury (SCI) treatment remains a formidable challenge, as current therapeutic approaches provide only marginal relief and fail to reverse the underlying tissue damage. This study aims develop novel composite material combining enzymatic nanoparticles nerve growth factor (NGF) modulate immune microenvironment enhance SCI repair. CeMn (NP) NP-polyethylene glycol (PEG) nanozymes were synthesized via sol–gel reaction DSPE-mPEG modification. Transmission Electron Microscopy, Selected-Area Diffraction, X-ray Diffraction Photoelectron Spectroscopy confirmed their crystalline structure, mixed-valence states, redox properties. Size uniformity, biocompatibility, catalytic activity assessed hydrodynamic diameter, zeta potential, elemental analysis. The Lightgel/NGF/CeMn NP-PEG was characterized electron microscopy, compression testing, rheological analysis, NGF release kinetics, 30-day degradation studies. Both in vitro vivo experiments conducted evaluate effects of on SCI. successfully synthesized, exhibiting favorable physical At concentration 4 µg/mL, maintained cell viability demonstrated enhanced biological activity. It also showed superior mechanical properties an effective profile. Notably, significantly upregulated expression growth-associated proteins, reduced inflammatory cytokines, scavenged reactive oxygen species (ROS), promoted M2 macrophage polarization by inhibiting cyclic GMP-AMP synthase (cGAS)-stimulator interferon genes (STING) signaling pathway. In rat model, it facilitated functional recovery attenuated inflammation. shows significant promise for SCI, effectively eliminating ROS, promoting polarization, reducing pro-inflammatory supporting neuronal regeneration. These substantially motor function rats, positioning promising candidate future clinical applications.

Язык: Английский

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Curcumin: A Golden Approach to Healthy Aging: A Systematic Review of the Evidence

Nutrients, Год журнала: 2024, Номер 16(16), С. 2721 - 2721

Опубликована: Авг. 15, 2024

Aging-related disorders pose significant challenges due to their complex interplay of physiological and metabolic factors, including inflammation, oxidative stress, mitochondrial dysfunction. Curcumin, a natural compound with potent antioxidant anti-inflammatory properties, has emerged as promising candidate for mitigating these age-related processes. However, gaps in understanding the precise mechanisms curcumin’s effects optimal dosages different conditions necessitate further investigation. This systematic review synthesizes current evidence on potential addressing disorders, emphasizing its impact cognitive function, neurodegeneration, muscle health older adults. By evaluating safety, efficacy, action curcumin supplementation, this aims provide insights into therapeutic promoting healthy aging. A search across three databases using specific keywords yielded 2256 documents, leading selection 15 clinical trials synthesis. Here, we highlight multifaceted agent combating disorders. The findings suggest that could offer effective approach enhancing quality life aging individuals. Further research well-designed are essential validate optimize use personalized medicine approaches conditions.

Язык: Английский

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GDF11 Mitigates Neuropathic Pain via Regulation of Microglial Polarization and Neuroinflammation through TGF-βR1/SMAD2/NF-κB Pathway in Male Mice

Journal of Neuroimmune Pharmacology, Год журнала: 2025, Номер 20(1)

Опубликована: Фев. 12, 2025

Язык: Английский

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Natural products for the treatment of ulcerative colitis: focus on the JAK/STAT pathway

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 26, 2025

Ulcerative colitis (UC) is an autoimmune disease with incompletely understood pathogenesis. The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway plays a key role in immune response inflammation. More more studies demonstrated that JAK/STAT associated the pathogenesis UC. affects UC multiple ways by regulating intestinal inflammatory response, affecting mucosal barrier, modulating T cell homeostasis, macrophages. Encouragingly, natural products are promising candidates for treatment Natural have advantage being multi-targeted rich therapeutic modalities. This review summarized research progress pathway-mediated Furthermore, latest on targeting were systematically summarized, including active ingredients such as arbutin, aloe polysaccharide, berberine, matrine, curcumin, Ginsenoside Rh2, so on. aim this paper to provide new ideas drug development regulate treating

Язык: Английский

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The Role and Application of M2 Macrophages in Spinal Cord Injury

Advances in Clinical Medicine, Год журнала: 2025, Номер 15(01), С. 981 - 989

Опубликована: Янв. 1, 2025

Язык: Английский

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Engineered hybrid exosomes responsive to reactive oxygen species target the treatment of spinal cord injury by repairing mitochondrial damage and promoting neuronal function recovery

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160669 - 160669

Опубликована: Фев. 1, 2025

Язык: Английский

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Tenascin-C Facilitates Microglial Polarization via TLR4/MyD88/NF-κB Pathway Following Subarachnoid Hemorrhage

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 3555 - 3570

Опубликована: Март 1, 2025

Purpose: This study primarily aims to elucidate the underlying mechanism of Tenascin-C in neuroinflammation and microglia polarization a mouse model subarachnoid hemorrhage (SAH). Methods: The was constructed by injecting blood into anterior chiasmatic cistern stimulating primary with hemoglobin vitro. Then, Imatinib mesylate used inhibit Tenascin-C. Through neurological function scoring, brain edema, cell extraction, immunofluorescence staining, CCK8, Tunel Elisa, Western blot other methods, potential induced explored. Results: results this observed that expression up-regulated after hemorrhage. Inhibiting increase imatinib could significantly ameliorate neuroinflammation, neuronal apoptosis, barrier disruption edema. When level decreased, numbers TLR4 positive, MyD88 positive NF-κB microglial cells decreased accordingly. Moreover, hemorrhage, number for M1-type markers increased significantly. After inhibited Tenascin-C, M2-type Conclusion: In summary, elevated induces activation microglia, releasing large inflammatory factors aggravating early injury. Keywords: SAH,

Язык: Английский

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Identification of novel biomarkers and immune characteristics of spinal cord injury based on comprehensive bioinformatic analysis: A retrospective observational study

NeuroMarkers., Год журнала: 2025, Номер 2(2), С. 100077 - 100077

Опубликована: Март 22, 2025

Язык: Английский

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Bioinformatics analysis techniques identify the ferroptosis-related gene MYC as a potential therapeutic target for spinal cord injury: an observational study based on the GEO database

Advanced technology in neuroscience ., Год журнала: 2025, Номер 2(2), С. 59 - 71

Опубликована: Апрель 8, 2025

JOURNAL/atin/04.03/02274269-202506000-00001/figure1/v/2025-04-06T104049Z/r/image-tiff Spinal cord injury is a destructive disease with limited effective treatment options. Ferroptosis, form of regulated cell death, has been suggested to be related the pathogenesis spinal injury. This study aimed elucidate prognostic value and therapeutic potential ferroptosis-related genes in using advanced bioinformatics techniques. We retrieved datasets from GEO database identified differentially expressed between control groups. These were intersected identify genes. A hub gene was through cytoHubba plugin Cytoscape, miRNA–mRNA–transcription factor drug–biomarker networks constructed reveal molecular targets compounds. The results revealed highly upregulated gene, MYC , group. Subsequent analyses 30 miRNAs, transcription factors, drugs as associated this for findings suggest that analysis techniques provide new insights into role injury, highlighting biomarker target lays foundation personalized facilitating development strategies tailored unique characteristics individual patients.

Язык: Английский

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Neuroinflammation and Amyotrophic Lateral Sclerosis: Recent Advances in Anti-Inflammatory Cytokines as Therapeutic Strategies

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3854 - 3854

Опубликована: Апрель 18, 2025

Neuroinflammation is an inflammatory response occurring within the central nervous system (CNS). The process marked by production of pro-inflammatory cytokines, chemokines, small-molecule messengers, and reactive oxygen species. Microglia astrocytes are primarily involved in this process, while endothelial cells infiltrating blood contribute to neuroinflammation when blood–brain barrier (BBB) damaged. increasingly recognized as a pathological hallmark several neurological diseases, including amyotrophic lateral sclerosis (ALS), closely linked neurodegeneration, another key feature ALS. In fact, neurodegeneration trigger for inflammation, neuroinflammation, turn, contributes motor neuron (MN) degeneration through induction synaptic dysfunction, neuronal death, inhibition neurogenesis. Importantly, resolution acute inflammation crucial avoiding chronic tissue destruction. Inflammatory processes mediated soluble factors known which both promoting inhibiting inflammation. Cytokines with anti-inflammatory properties may exert protective roles neuroinflammatory particular, interleukin (IL)-10, transforming growth factor (TGF)-β, IL-4, IL-13, IL-9 have been shown role CNS. Other recently emerging immune regulatory cytokines CNS include IL-35, IL-25, IL-37, IL-27. This review describes current understanding ALS highlights recent advances particular focus on their potential therapeutic applications Furthermore, we discuss strategies aimed at enhancing modulate disease.

Язык: Английский

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