Shared circulating diagnostic biomarkers and molecular mechanisms in ischemic stroke and systemic lupus erythematosus DOI Creative Commons

Xiaoyi Ma,

Lifei Huang, Huanhuan Yan

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 17, 2025

Introduction Ischemic stroke, a prevalent cerebrovascular disorder characterized by reduced cerebral blood flow, and systemic lupus erythematosus (SLE), an autoimmune disease affecting various organs, are suspected to share overlapping etiological mechanisms genetic predispositions. This study aimed identify shared diagnostic biomarkers molecular analyzing datasets from the GEO database. Methods We pinpointed differentially expressed genes using limma package identified co-expression modules associated with both conditions Weighted Gene Coexpression Network Analysis. Pathway enrichment analysis was conducted GO KEGG co-driver genes. LASSO regression applied evaluate potential markers, immune cell infiltration quantified CIBERSORT computational method. A middle artery occlusion (MCAO) mouse model developed assess core gene expression in vivo . Results 69 driver linked stroke SLE, which were narrowed down top 10 through Protein-Protein Interaction network Cytoscape. selected EIF2AK2, PARP9, IFI27 as biomarkers, supported ROC curve analysis. Immune profiles nearly identical between ischemic SLE. 9.4T MR imaging, H&E Nissl staining confirmed MCAO model, qPCR elevated of three hub Discussion Our findings provide evidence for common indicators offering novel insights therapeutic strategies targeting their microenvironments.

Язык: Английский

tRNA-derived small RNAs: their role in the mechanisms, biomarkers, and therapeutic strategies of colorectal cancer DOI Creative Commons
Bo Zhang, Yi Pan, Zhe Li

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 13, 2025

Colorectal cancer (CRC) is the third most prevalent malignancy and second leading cause of cancer-related mortality worldwide, with an increasing shift towards younger age onset. In recent years, there has been recognition significance tRNA-derived small RNAs (tsRNAs), encompassing fragments (tRFs) tRNA halves (tiRNAs). Their involvement in regulating translation, gene expression, reverse transcription, epigenetics gradually come to light. Emerging research revealed dysregulation tsRNAs CRC, implicating their role CRC initiation progression, highlighting potential early diagnosis, prognosis, therapeutic strategies. Although clinical application still its stages, findings highlight a close relationship between biogenesis function tsRNAs, chemical modifications, tumor immune microenvironment (TIME). Additionally, similar other RNAs, can be effectively delivered via nanoparticles (NPs). Consequently, future should focus on elucidating concerning base TIME regulation, immunotherapy, NPs delivery systems facilitate translation.

Язык: Английский

Процитировано

1
Machine Learning-Based Identification of Novel Exosome-Derived Metabolic Biomarkers for the Diagnosis of Systemic Lupus Erythematosus and Differentiation of Renal Involvement DOI
Zhongyu Wang, Wenjing Liu, Qiuheng Jin

и другие.

Current Medical Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Язык: Английский

Процитировано

0
Shared circulating diagnostic biomarkers and molecular mechanisms in ischemic stroke and systemic lupus erythematosus DOI Creative Commons

Xiaoyi Ma,

Lifei Huang, Huanhuan Yan

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 17, 2025

Introduction Ischemic stroke, a prevalent cerebrovascular disorder characterized by reduced cerebral blood flow, and systemic lupus erythematosus (SLE), an autoimmune disease affecting various organs, are suspected to share overlapping etiological mechanisms genetic predispositions. This study aimed identify shared diagnostic biomarkers molecular analyzing datasets from the GEO database. Methods We pinpointed differentially expressed genes using limma package identified co-expression modules associated with both conditions Weighted Gene Coexpression Network Analysis. Pathway enrichment analysis was conducted GO KEGG co-driver genes. LASSO regression applied evaluate potential markers, immune cell infiltration quantified CIBERSORT computational method. A middle artery occlusion (MCAO) mouse model developed assess core gene expression in vivo . Results 69 driver linked stroke SLE, which were narrowed down top 10 through Protein-Protein Interaction network Cytoscape. selected EIF2AK2, PARP9, IFI27 as biomarkers, supported ROC curve analysis. Immune profiles nearly identical between ischemic SLE. 9.4T MR imaging, H&E Nissl staining confirmed MCAO model, qPCR elevated of three hub Discussion Our findings provide evidence for common indicators offering novel insights therapeutic strategies targeting their microenvironments.

Язык: Английский

Процитировано

0