Cyclic GMP-AMP Synthase (cGAS) Deletion Promotes Less Prominent Inflammatory Macrophages and Sepsis Severity in Catheter-Induced Infection and LPS Injection Models DOI Open Access

Chatsuree Suksamai,

Warerat Kaewduangduen,

Pornpimol Phuengmaung

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5069 - 5069

Опубликована: Май 24, 2025

Activation of cGAS, a cytosolic receptor recognizing double-stranded DNA, in macrophages is important sepsis (a life-threatening condition caused by infection). The responses against induced subcutaneous implantation the Pseudomonas-contaminated catheters cGAS-deficient (cGAS−/−) mice were lower than wild-type (WT) as indicated liver enzymes, white blood cell count, cytokines, and M1-polarized spleens. Likewise, lethal dose lipopolysaccharide (LPS) less severe severity determined mortality, organ injury, cell-free serum cytokines. Patterns transcriptome (LPS)-stimulated bone marrow-derived clearly different between cGAS−/− WT cells. Gene set enrichment analysis (GSEA; computational statistical determination gene set) more prominent oxidative phosphorylation (OXPHOS; mitochondrial function) mTORC1 pathways LPS-activated compared with WT. Meanwhile, LPS upregulated cGAS increased cGAMP inducer) only along inflammation macrophages, supernatant pro-inflammatory molecules (nuclear factor kappa B; NF-κB), M1 polarization (IL-1β, CD80, CD86), macrophage extracellular traps (METs; web-like structures composed histones, other proteins) through detection citrullinated histone 3 (CitH3) immunofluorescent visualization. In conclusion, demonstrated (catheter-induced injection model) vitro (transcriptomic analysis, polarization, METs).

Язык: Английский

Establishment and identification of spleen cell line from tiger puffer fish(Takifugu rubripes)and immune transcriptome response to IHNV DOI

Yangbin He,

Jingjing Zhang,

Lei Lin

и другие.

Fish & Shellfish Immunology, Год журнала: 2025, Номер unknown, С. 110235 - 110235

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Cyclic GMP-AMP Synthase (cGAS) Deletion Promotes Less Prominent Inflammatory Macrophages and Sepsis Severity in Catheter-Induced Infection and LPS Injection Models DOI Open Access

Chatsuree Suksamai,

Warerat Kaewduangduen,

Pornpimol Phuengmaung

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5069 - 5069

Опубликована: Май 24, 2025

Activation of cGAS, a cytosolic receptor recognizing double-stranded DNA, in macrophages is important sepsis (a life-threatening condition caused by infection). The responses against induced subcutaneous implantation the Pseudomonas-contaminated catheters cGAS-deficient (cGAS−/−) mice were lower than wild-type (WT) as indicated liver enzymes, white blood cell count, cytokines, and M1-polarized spleens. Likewise, lethal dose lipopolysaccharide (LPS) less severe severity determined mortality, organ injury, cell-free serum cytokines. Patterns transcriptome (LPS)-stimulated bone marrow-derived clearly different between cGAS−/− WT cells. Gene set enrichment analysis (GSEA; computational statistical determination gene set) more prominent oxidative phosphorylation (OXPHOS; mitochondrial function) mTORC1 pathways LPS-activated compared with WT. Meanwhile, LPS upregulated cGAS increased cGAMP inducer) only along inflammation macrophages, supernatant pro-inflammatory molecules (nuclear factor kappa B; NF-κB), M1 polarization (IL-1β, CD80, CD86), macrophage extracellular traps (METs; web-like structures composed histones, other proteins) through detection citrullinated histone 3 (CitH3) immunofluorescent visualization. In conclusion, demonstrated (catheter-induced injection model) vitro (transcriptomic analysis, polarization, METs).

Язык: Английский

Процитировано

0