Cancer Cell International,
Год журнала:
2023,
Номер
23(1)
Опубликована: Окт. 16, 2023
Drug
resistance
is
a
major
obstacle
causing
chemotherapy
failure,
and
enabling
cancer
progression.
Exosome
excreted
by
cells
participated
in
progression
chemoresistance,
can
be
used
as
an
prognostic
biomarker.
Previous
studies
have
revealed
that
serum
exosomal
hsa-circ-0004771
over-expressed
colorectal
(CRC)
sufferers
suggested
it
predictive
biomarker
for
early
diagnosis
prognosis
of
CRC.
This
work
will
to
investigate
the
role
mechanism
mediating
5-fluorouracil
(5-FU)
CRC.Serum
tissue
samples
were
collected
from
60
patients
with
CRC/
benign
intestinal
disease,
healthy
control.
Exosomes
isolated
identified
cell
cultured
media
TEM,
WB,
NTA,
flow
cytometry.
qRT-PCR
WB
performed
evaluate
mRNA
expressions
has-circ-0004771
miR-653,
ZEB2
protein
expression,
respectively.
Cell
proliferation,
migration,
invasion,
apoptosis
abilities
assessed
BrdU
colony
formation
assay,
wound-healing
cytometry,
respectively.Exosomal
was
CRC
media,
while
miR-653
lower-expressed
tissues
cells.
Negative
correlations
existed
between
patients'
serum/cell
culture
tissues/cells,
Exosomal
positively
related
MiR-653
associated
poor
patients,
its
upregulation
restrained
migration
stimulated
apoptosis.
higher-expressed
5-FU-resistant
downregulated
overexpressed
In
vitro,
may
involved
5-FU-resistance
modulating
miR-653/ZEB2
pathway.miR-653
plays
tumour
suppressor
progression,
potentially
through
axis.
Abstract
Given
that
hypoxia
is
a
persistent
physiological
feature
of
many
different
solid
tumors
and
key
driver
for
cancer
malignancy,
it
thought
to
be
major
target
in
treatment
recently.
Tumor-associated
macrophages
(TAMs)
are
the
most
abundant
immune
cells
tumor
microenvironment
(TME),
which
have
large
impact
on
development
immunotherapy.
TAMs
massively
accumulate
within
hypoxic
regions.
represent
deadly
combination
because
has
been
suggested
induce
pro-tumorigenic
macrophage
phenotype.
Hypoxia
not
only
directly
affects
polarization,
but
also
an
indirect
effect
by
altering
communication
between
macrophages.
For
example,
can
influence
expression
chemokines
exosomes,
both
profound
impacts
recipient
cells.
Recently,
demonstrated
intricate
interaction
TME
relevant
poor
prognosis
increased
malignancy.
However,
there
no
comprehensive
literature
reviews
molecular
mechanisms
underlying
hypoxia-mediated
TAMs.
Therefore,
this
review
aim
collect
all
recently
available
data
topic
provide
insights
developing
novel
therapeutic
strategies
reducing
effects
hypoxia.
Frontiers in Immunology,
Год журнала:
2023,
Номер
13
Опубликована: Янв. 18, 2023
Ovarian
cancer
is
a
malignant
tumor
of
the
female
reproductive
system,
with
very
poor
prognosis
and
high
mortality
rates.
Chemotherapy
radiotherapy
are
most
common
treatments
for
ovarian
cancer,
unsatisfactory
results.
Exosomes
subpopulation
extracellular
vesicles,
which
have
diameter
approximately
30–100
nm
secreted
by
many
different
types
cells
in
various
body
fluids.
highly
stable
effective
carriers
immunotherapeutic
drugs.
Recent
studies
shown
that
exosomes
involved
cellular
responses
microenvironment,
influencing
development
therapeutic
efficacy
exhibiting
dual
roles
inhibiting
promoting
development.
also
contain
variety
genes
related
to
immunotherapy
could
be
potential
biomarkers
diagnosis
prognosis.
Undoubtedly,
great
field
immunotherapy.
However,
translation
this
idea
clinic
has
not
occurred.
Therefore,
it
important
understand
how
used
regulate
progression.
In
review,
we
summarize
fluids
mechanisms
influence
response.
We
discuss
prospects
clinical
application
exosome-based
cancer.
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
162, С. 114698 - 114698
Опубликована: Апрель 13, 2023
With
the
rapid
development
of
next-generation
sequencing
technology,
several
studies
have
shown
that
ncRNAs
can
act
as
competitive
endogenous
RNAs
(ceRNAs)
and
are
involved
in
various
biological
processes,
such
proliferation,
differentiation,
apoptosis,
migration
breast
cancer
(BC)
cells,
plays
an
important
role
BC
progression
a
molecular
target
for
its
diagnosis,
treatment,
prognosis,
differentiation
subtypes
age
groups
patients.
Based
on
description
ceRNA-related
functions,
this
study
screened
sorted
analysis
experimental
verification
conclusions
BC-related
ceRNAs
found
mediated
ceRNA
networks
promote
by
promoting
expression
genes
related
to
drug
resistance,
inducing
production
epithelial-mesenchymal
transition
(EMT)
metastasis
activating
cancer-related
signaling
pathways.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(12), С. 6480 - 6480
Опубликована: Июнь 9, 2022
Extracellular
vesicles
(EVs)
are
lipid
bilayer
membrane
particles
that
play
critical
roles
in
intracellular
communication
through
EV-encapsulated
informative
content,
including
proteins,
lipids,
and
nucleic
acids.
Mesenchymal
stem
cells
(MSCs)
pluripotent
with
self-renewal
ability
derived
from
bone
marrow,
fat,
umbilical
cord,
menstruation
blood,
pulp,
etc.,
which
they
use
to
induce
tissue
regeneration
by
their
direct
recruitment
into
injured
tissues,
the
heart,
liver,
lung,
kidney,
or
secreting
factors,
such
as
vascular
endothelial
growth
factor
insulin-like
factor.
Recently,
MSC-derived
EVs
have
been
shown
regenerative
effects
against
various
diseases,
partially
due
post-transcriptional
regulation
of
target
genes
miRNAs.
Furthermore,
garnered
attention
novel
drug
delivery
systems,
because
can
specially
encapsulate
molecules.
In
this
review,
we
summarize
molecular
mechanisms
EVs.
Biomarker Research,
Год журнала:
2023,
Номер
11(1)
Опубликована: Ноя. 19, 2023
Abstract
Tumor-associated
macrophages
(TAMs),
one
of
the
most
abundant
immune
cell
types
in
tumor
microenvironment
(TME),
account
for
approximately
50%
local
hematopoietic
cells.
TAMs
play
an
important
role
tumorigenesis
and
development
through
crosstalk
between
various
cells
cytokines
TME.
Exosomes
are
small
extracellular
vesicles
with
a
diameter
50–150
nm,
that
can
transfer
biological
information
(e.g.,
proteins,
nucleic
acids,
lipids)
from
secretory
to
recipient
circulatory
system,
thereby
influencing
progression
human
diseases,
including
cancer.
Recent
studies
have
suggested
TAMs-derived
exosomes
crucial
roles
malignant
proliferation,
invasion,
metastasis,
angiogenesis,
responses,
drug
resistance,
metabolic
reprogramming.
potential
be
targeted
therapy.
In
addition,
abnormal
expression
non-coding
RNAs
proteins
is
closely
related
clinicopathological
features
patients
cancer,
these
expected
become
new
liquid
biopsy
markers
early
diagnosis,
prognosis,
monitoring
tumors.
this
review,
we
explored
provide
diagnostic
biomarkers
therapeutic
targets
cancer
prevention.
The Indian Journal of Medical Research,
Год журнала:
2024,
Номер
159(2), С. 163 - 163
Опубликована: Янв. 1, 2024
From
an
initial
thought
of
being
used
as
a
cellular
garbage
bin
to
promising
target
for
liquid
biopsies,
the
role
exosomes
has
drastically
evolved
in
just
few
years
their
discovery
1983.
Exosomes
are
naturally
secreted
nano-sized
vesicles,
abundant
all
types
body
fluids
and
can
be
isolated
intact
even
from
stored
biological
samples.
Being
stable
carriers
genetic
material
(cellular
DNA,
mRNA
miRNA)
having
specific
cargo
(signature
content
originating
cells),
play
crucial
pathogenesis
have
been
identified
novel
source
biomarkers
variety
disease
conditions.
Recently
emerged
'liquid
biopsy
tool'and
shown
great
potential
field
non-invasive
diagnostics,
prognostics
treatment
response
monitoring
both
communicable
well
non-communicable
diseases.
However,
there
certain
limitations
overcome
which
restrict
use
exosome-based
gold
standard
testing
procedure
routine
clinical
practices.
The
present
review
summarizes
current
knowledge
on
tool
diagnosis,
prognosis
diseases
highlights
major
limitations,
technical
advancements
future
prospects
utilization
interventions.
Frontiers in Genetics,
Год журнала:
2023,
Номер
14
Опубликована: Фев. 28, 2023
Breast
cancer
is
the
most
prevalent
type
of
malignancy
among
women.
Exosomes
are
extracellular
vesicles
cell
membrane
origin
that
released
via
exocytosis.
Their
cargo
contains
lipids,
proteins,
DNA,
and
different
forms
RNA,
including
circular
RNAs.
Circular
RNAs
new
class
non-coding
with
a
closed-loop
shape
involved
in
several
types
cancer,
breast
cancer.
contained
lot
circRNAs
which
called
exosomal
circRNAs.
By
interfering
biological
pathways,
can
have
either
proliferative
or
suppressive
role
The
involvement
has
been
studied
consideration
to
tumor
development
progression
as
well
its
effects
on
therapeutic
resistance.
However,
exact
mechanism
still
unclear,
there
not
available
clinical
implications
exo-circRNAs
Here,
we
highlight
recent
potential
circRNAas
targets
diagnostics
for
Cancers,
Год журнала:
2023,
Номер
15(6), С. 1632 - 1632
Опубликована: Март 7, 2023
The
largest
part
of
human
DNA
is
transcribed
into
RNA
that
does
not
code
for
proteins.
These
non-coding
RNAs
(ncRNAs)
are
key
regulators
protein-coding
gene
expression
and
have
been
shown
to
play
important
roles
in
health,
disease
therapy
response.
Today,
endocrine
ERα-positive
breast
cancer
(BC)
a
successful
treatment
approach,
but
resistance
this
major
clinical
problem.
Therefore,
deeper
understanding
mechanisms
overcome
resistance.
An
increasing
amount
evidence
demonstrate
ncRNAs
affect
the
response
therapy.
Thus,
considered
versatile
biomarkers
predict
or
monitor
In
review
article,
we
intend
give
summary
update
on
effects
microRNAs
(miRNAs),
long
(lncRNAs)
circular
(circRNAs)
estrogen
signaling
BC
cells,
pathway
being
target
therapy,
their
role
For
purpose,
reviewed
articles
these
topics
listed
PubMed
database.
Finally,
provide
an
assessment
regarding
use
ncRNA
types,
particularly
circulating
forms,
as
predictive
potential
targets
Biotechnology Journal,
Год журнала:
2024,
Номер
19(2)
Опубликована: Фев. 1, 2024
Abstract
Exosomes
have
been
the
hidden
treasure
of
cell
in
terms
cellular
interactions,
transportation
and
therapy.
The
native
exosomes
(NEx)
secreted
by
parent
cells
hold
promising
aspects
cancer
diagnosis
NEx
has
low
immunogenicity,
high
biocompatibility,
toxicity
stability
which
enables
them
to
be
an
ideal
prognostic
biomarker
diagnosis.
However,
due
heterogeneity,
lacks
specificity
accuracy
used
as
therapeutic
drug
delivery
vehicle
Transforming
these
with
their
innate
structure
multiple
receptors
engineered
(EEx)
can
provide
better
opportunities
field
theranostics.
surface
exhibits
numeric
modified
pave
direction
its
Through
membrane,
EEx
increased
loading
potentiality
higher
target
act
a
nanocarrier
for
delivery.
This
review
provides
insights
into
tool
along
need
transformation
We
also
highlighted
different
methods
associated
transformations,
nano‐bio
interaction
recipient
major
challenges
clinical
application