Fractal and Fractional,
Год журнала:
2024,
Номер
9(1), С. 9 - 9
Опубликована: Дек. 28, 2024
By
analyzing
reconstructed
three-dimensional
images
of
retinal
bipolar
neurons,
we
show
that
their
dendritic
arbors
weave
through
space
in
a
manner
generates
fractal-like
behavior
quantified
by
an
‘effective’
fractal
dimension.
Examining
this
along
with
traditional
morphological
parameters
reveals
dependence
arbor
dimension
on
the
summation
lengths
arbor’s
dendrites.
We
discuss
implications
for
healthy
neurons
and
also
deterioration
unhealthy
response
to
diseases.
Journal of Neuroinflammation,
Год журнала:
2025,
Номер
22(1)
Опубликована: Янв. 28, 2025
Abstract
Background
The
retinal
degenerative
diseases
retinitis
pigmentosa
(RP)
and
atrophic
age-
related
macular
degeneration
(AMD)
are
characterized
by
vision
loss
from
photoreceptor
(PR)
degeneration.
Unfortunately,
current
treatments
for
these
limited
at
best.
Genetic
other
preclinical
evidence
suggest
a
relationship
between
inflammation.
To
further
explore
this
relationship,
we
tested
whether
Ibuprofen
(IBU),
an
FDA-approved
non-steroidal
anti-inflammatory
drug
(NSAID),
could
promote
PR
survival
function
in
mouse
model
of
light
damage
(LD)-induced
Methods
LD
was
induced
exposing
mice
to
4000
lx
2–4
hours
(h).
IBU
(100
or
200
mg/kg)
vehicle
administered
daily
intraperitoneal
injection.
Retinal
structure
were
evaluated
spectral-domain
optical
coherence
tomography
(SD-OCT)
electroretinography
(ERG).
Cell
death
genes
analyzed
24
72
h
after
using
the
Mouse
Pan-Cell
Death
Pathway
PCR
Array
(88
genes).
cellular
location
protein
expression
key
necroptosis
assessed
immunohistochemistry.
Results
outer
nuclear
layer
(ONL)
thickness
vehicle-injected
animals
8.7
±
0.6%
retinas
without
(
p
<
0.0001).
In
mg/kg
treated
mice,
central
ONL
74.9
7.7%
untreated
0.001).
A-wave
b-wave
ERG
amplitudes
significantly
preserved
IBU-treated
animals.
inhibited
Twenty-four
hour
LD,
mRNA
inflammatory-factors
tumor
necrosis
factor
Tnf
),
interleukin-1
beta
Il1B
C-C
motif
chemokine
ligand
2
Ccl2
)
increased
10-,
17-,
533-fold,
respectively;
animals,
levels
inflammatory
factors
not
different
no-LD
controls.
Expression
genes,
including
Ripk3
Mlkl
,
upregulated
vehicle-treated
but
dramatically
reduced
near
no
mice.
Microglia
activation
MLKL
upregulation
observed
primarily
photoreceptors
12
as
microglia
migration
plexiform
(OPL)
retinas.
Conclusions
Systemic
administration
partially
protected
light-induced
photochemical
both
inflammation
cell
pathways.
Our
results
that
NSAIDs
may
provide
promising
therapeutic
approach
treatment
human
diseases.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3806 - 3806
Опубликована: Апрель 17, 2025
Pyridazin-3(2H)one-based
molecules
have
always
attracted
the
attention
of
medicinal
chemists
due
to
their
different
pharmacological
properties.
The
incorporation
such
nuclei
in
therapeutically
active
either
as
monocyclic
units
or
fused
bi-
tricyclic
scaffolds
results
a
wide
range
effects
anti-inflammatory,
analgesic,
anticancer,
antimicrobial,
antiviral,
cardiovascular-protective,
antiulcer,
and
many
other
useful
activities.
In
accordance
with
our
consolidated
experience
gained
over
years
chemistry
biology
pyridazin-3(2H)ones,
this
review
summarizes
SAR
studies
pyridazinone-based
polycyclic
compounds
endowed
various
biological
therapeutic
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(2), С. 1253 - 1253
Опубликована: Янв. 19, 2024
This
retrospective
study
examines
the
clinical
characteristics
and
underlying
genetic
variants
that
exist
in
a
Leber
congenital
amaurosis
(LCA)
patient
cohort
evaluated
at
inherited
retinal
disease
(IRD)
clinic
University
of
Minnesota
(UMN)/M
Health
System.
Our
LCA
consisted
33
non-syndromic
patients
one
with
Joubert
syndrome.
We
report
their
relevant
history,
findings,
testing
results.
monitored
presentation
utilizing
ocular
coherence
tomography
(OCT)
fundus
autofluorescence
(FAF).
Electroretinogram
(ERG)
was
performed
when
clinically
indicated.
Next-generation
sequencing
(NGS)
counseling
offered
to
all
patients.
Advanced
photoreceptor
loss
noted
85.7%
subjects.
All
who
underwent
FAF
had
findings
either
ring
macular
hypo/hyper
AF
or
peripheral
hypo-AF.
abnormal
ERG
findings.
A
diagnostic
test
result
identified
74.2%
via
NGS
single-gene
panel
testing.
Two
our
qualified
for
Luxturna
Cells,
Год журнала:
2023,
Номер
12(16), С. 2043 - 2043
Опубликована: Авг. 10, 2023
HK2
and
PKM2
are
two
main
regulators
of
aerobic
glycolysis.
Photoreceptors
(PRs)
use
glycolysis
to
produce
the
biomass
necessary
for
daily
renewal
their
outer
segments.
Previous
work
has
shown
that
important
normal
function
long-term
survival
PRs
but
dispensable
PR
maturation,
individual
loss
opposing
effects
on
during
acute
nutrient
deprivation.
We
generated
double
conditional
(dcKO)
mice
lacking
expression
in
rod
PRs.
Western
blotting,
immunofluorescence,
optical
coherence
tomography,
electroretinography
were
used
characterize
phenotype
dcKO
animals.
Targeted
stable
isotope
tracing
metabolomics,
qRT-PCR,
retinal
oxygen
consumption
performed.
show
animals
displayed
early
shortening
inner/outer
segments,
followed
by
with
aging,
much
more
rapidly
than
either
knockout
alone
without
functional
as
measured
ERG.
Significant
alterations
central
glucose
metabolism
observed
any
apparent
changes
mitochondrial
function,
prior
degeneration.
Finally,
following
experimental
detachment
was
unchanged
compared
wild-type
These
data
suggest
have
differing
roles
promoting
neuroprotection
identifying
them
implications
developing
therapeutic
options
combating
disease.
Molecular Metabolism,
Год журнала:
2024,
Номер
88, С. 101994 - 101994
Опубликована: Июль 19, 2024
Retinitis
pigmentosa
(RP)
is
a
hereditary
retinal
disease
characterized
by
progressive
photoreceptor
degeneration,
leading
to
vision
loss.
The
best
hope
for
cure
RP
lies
in
gene
therapy.
However,
given
that
patients
are
most
often
diagnosed
the
midst
of
ongoing
it
unknown
how
proteome
changes
as
progresses,
and
which
can
be
prevented,
halted,
or
reversed
