Dinaciclib Interrupts Cell Cycle and Induces Apoptosis in Oral Squamous Cell Carcinoma: Mechanistic Insights and Therapeutic Potential

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2197 - 2197

Опубликована: Фев. 28, 2025

Dinaciclib, a potent cyclin-dependent kinase (CDK) inhibitor, has demonstrated considerable antitumor effects in various malignancies. However, its impact on oral squamous cell carcinoma (OSCC), predominant and highly aggressive form of head neck (HNSC) with limited treatment options, remains underexplored. We conducted gene set enrichment analyses HNSC patients that reinforced the relevance these cycle-related genes to OSCC pathogenesis. Given known dysregulation patients, we hypothesized Dinaciclib may inhibit growth by targeting overexpressed cyclins CDKs, thereby disrupting cycle progression inducing apoptosis. This study investigated Dinaciclib's proliferation, progression, apoptosis lines Ca9-22, OECM-1, HSC-3. Our results significantly reduces proliferation dose-dependent manner. Flow cytometry Western blot showed induces arrest at G1/S G2/M transitions downregulating Cyclins A, B, D, E, along CDKs 1 2-key regulators checkpoints. Furthermore, upregulated apoptotic markers, such as cleaved-caspase-3 cleaved-PARP, confirming pro-apoptotic effects. In conclusion, findings highlight therapeutic promise simultaneously These support further exploration viable monotherapy or combination other subtypes improve patient outcomes.

Язык: Английский

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Metformin as an immunomodulatory agent in enhancing head and neck squamous cell carcinoma therapies

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2025, Номер 1880(2), С. 189262 - 189262

Опубликована: Янв. 17, 2025

Язык: Английский

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Gene Editing in ErbB/HER Family-Mediated Cancer Immunology

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

The ErbB/HER family has an essential role in tumor progression, proliferation, invasion, metastasis, and migration. ErbB/HER-targeted therapeutic agents have emerged as effective options to achieve excellent clinical outcomes boost cancer drug discovery by enhancing treatment efficacy, lowering resistance, minimizing systemic toxicity. Furthermore, combination therapy targeting members, well hormonal therapy, chemotherapy, immunotherapy, radiotherapy, also enhances effects for immunology. Zinc-finger nucleases (ZFNs), transcription activator-like effector (TALENs), Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Associated 9 (CRISPR-Cas9) comprise powerful tools redefining the boundaries of research. In this chapter, we provide a comprehensive evaluation anti-cancer single combined therapeutics target which could represent promising approaches treatment. We discuss recent worldwide advancements structures, mechanism, selectivity, efficacy design development efforts, sheds light on their potential improving addition, highlight achievements potentials ZFNs, TALENs, CRISPR/Cas9 immunology, such genetic analysis manipulation. customized application CRISPR/Cas9-mediated ErbB2/HER2 inhibited cell proliferation tumorigenicity opens up novel possibility

Язык: Английский

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NDUFS1 promotes malignant breast cancer behaviors through activation of mitochondrial metabolism and PROX1/c-Myc signaling

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

Breast cancer is the most prevalent among women. Previous studies demonstrated that adipose-derived stem cells (ADSCs) co-cultured with resistin promote malignant behaviors in breast cells. This study explores roles of ADSCs and adipocytokine within tumor microenvironment, emphasizing their contributions to metabolic reprogramming progression. RNA sequencing analysis pathways revealed co-culture resistin-treated exhibited elevated expression NDUFS1, largest subunit mitochondrial complex I. Knockdown NDUFS1 inhibited cell proliferation tumorsphere formation, whereas its overexpression enhanced these effects through metabolism-mediated PROX1/c-Myc signaling pathway. Furthermore, treatment metformin, an inhibitor NDUFS1-activated metabolism, reduced Myc PROX1 diminished proliferation. Syngeneic orthotopic mouse model showed downregulation significantly mammary growth alongside decreased c-Myc. Also, metabolism inhibitors metformin rotenone a therapeutic effect on NDUFS1-expressing tumor. Collectively, this research establishes novel mechanistic framework linking adaptations cancer, paving way for innovative strategies aimed at targeting signaling.

Язык: Английский

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Collision of functional MOFs enable label-free amperometry biosensor: A scalable ATP detection platform for the study of stomatology disease

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113462 - 113462

Опубликована: Март 1, 2025

Язык: Английский

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Auricularia auricula polysaccharides alleviate experimental silicosis by targeting EGFR through the “gut-lung axis”

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142541 - 142541

Опубликована: Март 1, 2025

Язык: Английский

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Advances in Electrochemical Biosensors for the Detection of Common Oral Diseases

Critical Reviews in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 1 - 21

Опубликована: Фев. 16, 2024

Limiting and preventing oral diseases remains a major challenge to the health of populations around world, so finding ways detect early-stage (e.g., caries, periodontal disease, cancer) aiding in their prevention has always been an important clinical treatment concept. The development application electrochemical detection technology can provide support for early non-invasive diagnosis make up shortcomings traditional diagnostic methods, which are highly sensitive, non-invasive, cost-effective, less labor-intensive. It detects specific disease markers body fluids through reactions, discovers warning signals diseases, realizes rapid reliable diagnosis. This paper comprehensively summarizes biosensors common terms platforms, sensing types, detection, discusses challenges faced by as well great prospects future applications, hope providing insights diseases.

Язык: Английский

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The role of the SOX2 gene in cervical cancer: focus on ferroptosis and construction of a predictive model

Journal of Cancer Research and Clinical Oncology, Год журнала: 2024, Номер 150(12)

Опубликована: Ноя. 23, 2024

Язык: Английский

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2α, 3α, 24‐Thrihydroxyurs‐12‐en‐24‐ursolic acid enhances the cytotoxic effect of cisplatin on oral cancer cells by down‐regulating autophagy

Journal of Cellular Biochemistry, Год журнала: 2023, Номер 125(2)

Опубликована: Ноя. 22, 2023

Abstract This study aimed to investigate the effect and mechanism of 2α, 3α, 24‐thrihydroxyurs‐12‐en‐24‐ursolic acid (TEOA) alone or in combination with cisplatin on oral cancer. TEOA, a pentacyclic triterpenoid compound isolated from roots Actinidia eriantha , has demonstrated antitumor activity preclinical experiments. However, its role cancer remains poorly understood. Our findings revealed that low concentration TEOA did not exhibit significant cytotoxicity against squamous cell carcinoma cells. when combined cisplatin, showed therapeutic effect. The treatments resulted inhibition proliferation migration increase apoptosis Cisplatin exposure increased autophagy levels, which may contribute chemoresistance. Of note, presence significantly inhibited cisplatin‐induced autophagy, leading improved chemotherapy efficacy. indicate mild dosage enhance cytotoxic by downregulating

Язык: Английский

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The Role of SOX 2 Gene in Cervical Cancer: Focus on Iron Death and Construct a Predictive Model

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 19, 2024

Background The intricate interplay between stemness markers and cell death pathways significantly influences the pathophysiology of cervical cancer. SOX2, a pivotal regulator stem pluripotency, has recently been implicated in modulation ferroptosis, specialized form iron-dependent death, cancer dynamics. This study delineates role SOX2 ferroptotic landscape carcinoma. Objective To delineate association expression ferroptosis develop robust, SOX2-centric predictive model for patient prognosis, enhancing personalized therapeutic approaches. Methods A multi-dimensional approach integrating advanced bioinformatics, comprehensive molecular profiling, state-of-the-art machine learning algorithms was employed to assess patterns their correlation with tissues. prognostic meticulously crafted, incorporating levels indicators. Results investigation unveiled profound processes cancer, substantiated by robust evidence. developed model, underpinned expression, exhibited superior accuracy potentially guiding decision-making. Conclusion underscores critical orchestrating pathway presenting novel framework. emerges as significant advancement evaluation, offering gateway precision oncology realm gynecologic oncology.

Язык: Английский

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