BMC Gastroenterology, Год журнала: 2025, Номер 25(1)
Опубликована: Апрель 8, 2025
Язык: Английский
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Янв. 27, 2025
Mitochondrial function is crucial for hepatic lipid metabolism. Current research identifies two types of mitochondria based on their contact with droplets: peridroplet (PDM) and cytoplasmic (CM). This work aimed to investigate the alterations CM PDM in metabolic dysfunction-associated steatotic liver disease (MASLD) induced by spontaneous type-2 diabetes mellitus (T2DM) db/db mice. It was found that insulin resistance increased both number size droplets enhancing accumulation free fatty acids, which accompanied an increase contacts mitochondria. We described different patterns tight between small purified examining oxidation states morphological characteristics. In CM, enhanced acid resulted elongated surrounded single were responsible droplet consumption, while PDM, substrates synthesis promoted expansion assistance endoplasmic reticulum. These data show ways mitochondrial could provide new insights future
Язык: Английский
Процитировано
0
Journal of Food Biochemistry, Год журнала: 2025, Номер 2025(1)
Опубликована: Янв. 1, 2025
Nonalcoholic fatty liver disease (NAFLD) has become a global pandemic, imposing significant socioeconomic burden. Hepatic steatosis is key pathological event in NAFLD. However, there still lack of effective drugs for NAFLD treatment. Liquiritin (LIQ), derived from licorice root, exhibits antioxidative and anti‐inflammatory properties, making it valuable managing various conditions such as dermatological disorders, respiratory ailments, gastritis. Here, we investigated the impact underlying mechanisms LIQ on hepatic mouse primary hepatocytes (MPHs). Our study found that significantly decreased palmitic acid (PA)‐induced lipid accumulation MPHs. Additionally, remarkably increased mRNA expression Cpt1a, PPAR α , Ehhadh, Cyp4a10, Acox1, suggesting achieves its lipid‐lowering effect by regulating mitochondria‐mediated β‐oxidation. Furthermore, regulatory mitochondrial oxidative phosphorylation (OXPHOS) process was confirmed Seahorse analysis. Mechanistically, bioinformatics analysis predicted VEGFA crucial target mediating beneficial effects against PA‐induced Notably, treatment with purified protein partially counteracted properties LIQ. our data showed promoted degradation through ubiquitin–proteasome pathway. Therefore, findings not only confirm MPHs but also identify potential target. These results highlight therapeutic promise introduce novel treating steatosis.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 306, С. 141660 - 141660
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Antioxidants, Год журнала: 2025, Номер 14(4), С. 372 - 372
Опубликована: Март 21, 2025
Mitochondria are of great importance in cell biology since they major sites adenosine triphosphate (ATP) production and widely involved different cellular pathways the response to stress. During ATP production, reactive oxygen species (ROS) can be produced. While a small amount ROS may important for regulation physiological processes, at elevated levels turn into harmful agents leading damage. From pathological perspective, it could particularly interesting focus on mitochondrial function endothelial cells development aging onset diseases, including renal, cardio-metabolic, liver neurodegenerative ones. However, date, there no surveys which address above issues. To fill this gap, valuable collect recent findings about role mitochondria function, not only increase knowledge but also clinical applications. Here, we overview most issues view characterizing as an innovative potential target prevention aging, well treatment conditions.
Язык: Английский
Процитировано
0
Nutrients, Год журнала: 2025, Номер 17(7), С. 1214 - 1214
Опубликована: Март 30, 2025
Nonalcoholic fatty liver disease (NAFLD) is a growing global health concern that closely linked to metabolic syndrome, yet no approved pharmacological treatment exists. The Mediterranean diet (MD) emerged as first-line dietary intervention for NAFLD, offering and hepatoprotective benefits. Now conceptualized complex chemical matrix rich in bioactive compounds, the MD exerts antioxidant anti-inflammatory effects, improving insulin sensitivity lipid metabolism. Mitochondria play central role NAFLD pathophysiology, influencing energy metabolism, oxidative stress, homeostasis. Emerging evidence suggests MD's compounds enhance mitochondrial function by modulating phosphorylation, biogenesis, mitophagy. However, most research has focused on individual rather than whole, leaving gaps understanding its collective impact pattern. This narrative review explores how influence highlighting key pathways such substrate control, dynamics, efficiency. A literature search was conducted identify relevant studies MD, mitochondria, NAFLD. While promising, our remains incomplete, particularly when current knowledge limited lack of mechanistic comprehensive holistic impact. Future integrating cutting-edge experimental approaches needed elucidate intricate diet-mitochondria interactions. deeper influences essential developing precision-targeted nutritional strategies can effectively prevent manage disease.
Язык: Английский
Процитировано
0
Toxicon, Год журнала: 2025, Номер unknown, С. 108338 - 108338
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)
Опубликована: Фев. 27, 2024
Abstract Background Mitochondrial alterations, often dependent on unbalanced mitochondrial dynamics, feature in the pathobiology of human cancers, including multiple myeloma (MM). Flavanones are natural flavonoids endowed with targeting activities. Herein, we investigated capability Hesperetin (Hes) and Naringenin (Nar), two aglycones Hesperidin Naringin flavanone glycosides, to selectively target Drp1, a pivotal regulator prompting anti-MM activity. Methods Molecular docking analyses were performed crystallographic structure Dynamin-1-like protein (Drp1), using Hes Nar molecular structures. Cell viability apoptosis assessed MM cell lines, or co-culture systems primary bone marrow stromal cells, Titer Glo Annexin V-7AAD staining, respectively; clonogenicity was determined methylcellulose colony assays. Transcriptomic carried out Ion AmpliSeq™ platform; mRNA expression levels by quantitative RT-PCR western blotting, respectively. architecture transmission electron microscopy. Real time measurement oxygen consumption high resolution respirometry living cells. In vivo anti-tumor activity evaluated NOD-SCID mice subcutaneously engrafted Results found accommodate within GTPase binding site inhibit Drp1 activity, leading hyperfused mitochondria reduced OXPHOS. vitro, viability, even presence patient-derived triggering ER stress apoptosis. Interestingly, rewired metabolism through down-regulation master transcriptional activators (SREBF-1, c-MYC) lipogenesis genes. An extract Tacle, Citrus variety rich Naringin, capable recapitulate phenotypic perturbations each flavanone, vivo. Conclusion proliferation, rewire induce cells via antagonism fission driver Drp1. These results provide framework for development therapeutics aberrant dependencies.
Язык: Английский
Процитировано
4
Biomolecules, Год журнала: 2025, Номер 15(2), С. 163 - 163
Опубликована: Янв. 22, 2025
Metabolic dysfunction-associated steatotic liver disease (MASLD) is correlated with an increased cardiovascular risk, independent of other traditional risk factors. The mechanisms underlying this pathogenic link are complex yet remain incompletely elucidated. Among these, the most significant visceral adiposity, low-grade inflammation and oxidative stress, endothelial dysfunction, prothrombotic status, insulin resistance, dyslipidemia postprandial hyperlipemia, gut dysbiosis, genetic mutations. Cardiovascular diseases leading cause death in patients MASLD. These have incidence coronary artery disease, carotid structural functional cardiac abnormalities, valvulopathies, as well arrhythmias conduction disorders. In review, we present latest data on association between MASLD focusing that explain correlation these two pathologies. Given high rates morbidity mortality among MASLD, consider it imperative to raise awareness risks associated condition within general population. Further research essential clarify linked This understanding may facilitate identification new diagnostic prognostic biomarkers for patients, novel therapeutic targets.
Язык: Английский
Процитировано
0
Molecular Biology Reports, Год журнала: 2025, Номер 52(1)
Опубликована: Янв. 28, 2025
Язык: Английский
Процитировано
0
BMC Gastroenterology, Год журнала: 2025, Номер 25(1)
Опубликована: Янв. 30, 2025
Neuregulin (NRG) family is involved in energy metabolism, among which NRG1 a neuregulin proved to play protective role MAFLD cells. But the presice echanism has not been fully illustrated. This study aimed investigate of via ERK/SIRT1 signaling pathogenesis MAFLD. C57BL/6 mice were fed with high-fat diet for 8 weeks, and then injected (0.3 mg/kg/d) PD98059 tail vein 5 weeks. HepG2 cells induced by oleic acid palmitic treated 20ng/mL 10µmol/L PD98059. The changes histopathological, biochemical indexes, inflammatory factors, lipid apoptosis autophagy parameters measured. expressions cell animal models significantly lower than that control group. After intervention ERK inhibitor PD98059, expression decreased vivo, but no significant change was observed vitro. Moreover, ameliorated hepatic steatosis, enhanced viability, reduced apoptosis, attenuated liver injury both vitro vivo. intervention, ERBB2, ERBB3, p-ERK1/2, SIRT1 p-FOXO1 as well LC3II/I ratio tissues increased, while SREBP1c decreased. aforementioned therapeutic effect lost after might activating downstream ERK1/2 through ErbB2-ErbB3, promotes markers. indicate new strategy
Язык: Английский
Процитировано
0