Exosomes as targeted diagnostic biomarkers: Recent studies and trends
Life Sciences,
Год журнала:
2024,
Номер
354, С. 122985 - 122985
Опубликована: Авг. 14, 2024
Язык: Английский
Parkinson’s Spectrum Mechanisms in Pregnancy: Exploring Hypothetical Scenarios for MSA in the Era of ART
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3348 - 3348
Опубликована: Апрель 3, 2025
Multiple
System
Atrophy
(MSA)
is
a
rare,
rapidly
progressive
neurodegenerative
disorder
marked
by
autonomic
dysfunction,
parkinsonism,
and
cerebellar
ataxia.
While
predominantly
affecting
individuals
in
their
fifth
or
sixth
decade,
advancements
assisted
reproductive
technologies
(ART)
have
created
new
clinical
scenarios
involving
pregnancies
women
within
MSA's
typical
onset
range.
Given
the
scarcity
of
documented
MSA
pregnancies,
this
review
leverages
insights
from
related
Parkinson's
spectrum
mechanisms
to
explore
hypothetical
for
how
pregnancy-induced
physiological
changes
might
influence
progression.
Pregnancy-induced
hormonal
fluctuations,
including
elevated
estrogen
progesterone
levels,
may
modulate
α-synuclein
aggregation
neuroinflammatory
pathways.
Immune
adaptations,
such
as
fetal
microchimerism
Th2-biased
immune
profiles,
introduce
additional
complexities,
particularly
donor
embryo
complex
microchimerism.
Metabolic
demands
oxidative
stress
further
intersect
with
these
mechanisms,
potentially
accelerating
disease
We
analyze
existing
literature
theoretical
models,
emphasizing
need
interdisciplinary
research.
Clinical
implications
are
discussed
propose
evidence-based
strategies
optimizing
maternal-fetal
outcomes.
This
paper
identifies
critical
knowledge
gaps
proposes
avenues
future
investigation
optimize
outcomes
unique
underexplored
intersection.
Язык: Английский
Progranulin deficiency in the brain: the interplay between neuronal and non-neuronal cells
Translational Neurodegeneration,
Год журнала:
2025,
Номер
14(1)
Опубликована: Апрель 16, 2025
Abstract
Heterozygous
mutations
in
GRN
gene
lead
to
insufficient
levels
of
the
progranulin
(PGRN)
protein,
resulting
frontotemporal
dementia
(FTD)
with
TAR
DNA-binding
protein
43
(TDP-43)
inclusions,
classified
pathologically
as
lobar
degeneration
(FTLD-TDP).
Homozygous
are
exceedingly
rare
and
cause
neuronal
ceroid
lipofuscinosis
11,
a
lysosomal
storage
disease
onset
young
adulthood,
or
an
FTD
syndrome
late-onset
manifestations.
In
this
review,
we
highlight
broad
spectrum
clinical
phenotypes
associated
PGRN
deficiency,
including
primary
progressive
aphasia
behavioral
variant
dementia.
We
explore
these
alongside
relevant
rodent
vitro
human
models,
ranging
from
induced
pluripotent
stem
cell-derived
neural
progenitors,
neurons,
microglia,
astrocytes
genetically
engineered
heterotypic
organoids
containing
both
neurons
astrocytes.
summarize
advantages
limitations
models
recapitulating
main
FTLD-
hallmarks,
highlighting
role
non-cell-autonomous
mechanisms
formation
TDP-43
pathology,
neuroinflammation,
neurodegeneration.
Data
obtained
patients’
brain
tissues
biofluids,
parallel
single-cell
transcriptomics,
demonstrate
complexity
interactions
among
highly
heterogeneous
cellular
clusters
present
brain,
astrocytes,
oligodendroglia,
endothelial
cells,
pericytes.
Emerging
evidence
has
revealed
that
deficiency
is
cell
cluster-specific,
often
conserved,
genetic
molecular
central
nervous
system.
focus
on
how
distinct
populations
their
dysfunctional
crosstalk
contribute
neurodegeneration
neuroinflammation
FTD-
.
Specifically,
characterize
lipid
droplet-accumulating
microglia
alterations
myelin
content
dysfunction
caused
by
deficiency.
Additionally,
consider
deregulation
glia-neuron
communication
affects
exchange
organelles
such
mitochondria,
removal
excess
toxic
products
aggregates,
PGRN-related
Язык: Английский
Exosome nanovesicles: biomarkers and new strategies for treatment of human diseases
MedComm,
Год журнала:
2024,
Номер
5(8)
Опубликована: Июль 15, 2024
Exosomes
are
nanoscale
vesicles
of
cellular
origin.
One
the
main
characteristics
exosomes
is
their
ability
to
carry
a
wide
range
biomolecules
from
parental
cells,
which
important
mediators
intercellular
communication
and
play
an
role
in
physiological
pathological
processes.
have
advantages
biocompatibility,
low
immunogenicity,
biodistribution.
As
researchers'
understanding
has
increased,
various
strategies
been
proposed
for
use
diagnosing
treating
diseases.
Here,
we
provide
overview
biogenesis
composition
exosomes,
describe
relationship
between
disease
progression,
focus
on
as
biomarkers
early
screening,
monitoring,
guiding
therapy
refractory
diseases
such
tumors
neurodegenerative
We
also
summarize
current
applications
especially
engineered
efficient
drug
delivery,
targeted
therapies,
gene
immune
vaccines.
Finally,
challenges
potential
research
directions
clinical
application
discussed.
In
conclusion,
emerging
molecule
that
can
be
used
diagnosis
treatment
diseases,
combined
with
multidisciplinary
innovative
solutions,
will
applications.
Язык: Английский
Multiomics analysis of platelet-rich plasma promoting biological performance of mesenchymal stem cells
BMC Genomics,
Год журнала:
2024,
Номер
25(1)
Опубликована: Июнь 5, 2024
Abstract
Mesenchymal
Stem
Cells
are
ideal
seed
cells
for
tissue
repair
and
cell
therapy
have
promising
applications
in
regenerative
medicine
engineering.
Using
Platelet-Rich
Plasma
as
an
adjuvant
to
create
improve
the
microenvironment
growth
can
enhance
biological
properties
of
efficacy
therapy.
However,
mechanism
by
which
improves
performance
is
still
unknown.
In
this
study,
examining
effects
on
Cells,
combined
with
multiomics
analysis
(Transcriptomics,
Proteomics
Metabolomics)
related
tests,
we
analyzed
specific
pathways,
mechanisms
metabolic
pathways
Cells.
vitro
culture
system,
was
significantly
improved
after
replacing
Foetal
Bovine
Serum
Plasma,
genes
(
ESM1
,
PDGFB
CLEC7A
CCR1
ITGA6
et
al.
)
proliferation,
adhesion,
growth,
migration
signal
transduction
were
upregulated.
secretion
function
MSC
exosomes,
upregulate
many
proteins
repair,
immune
regulation
anti-infection,
effect
exosomes
skin
injury.
After
underwent
reprogramming,
metabolism
amino
acids
fatty
various
signaling
changed,
anabolic
enhanced.
These
results
provide
a
theoretical
technical
reference
optimizing
improving
characteristics
clinical
application
Язык: Английский
Circular RNAs: novel noncoding players in male infertility
Hereditas,
Год журнала:
2024,
Номер
161(1)
Опубликована: Ноя. 18, 2024
Abstract
Infertility
is
a
global
problem
being
associated
with
emotional
and
financial
burden.
Recent
studies
have
shown
contribution
of
group
non-coding
RNAs,
namely
circular
RNAs
(circRNAs)
to
the
etiology
some
infertility
conditions.
CircRNA
are
transcribed
from
exons
form
RNA
molecule,
abundant
in
eukaryotes.
Traditionally
classified
as
RNA,
these
transcripts
endogenously
produced
through
either
non-canonical
back-splicing
or
linear
splicing,
typically
precursor
messenger
ribonucleic
acid
(pre-mRNA).
While
during
canonical
splicing
process
3’
end
exon
joined
5’
succeeding
mRNA,
backsplicing,
same
make
molecule.
circRNAs
involved
regulation
several
aspects
spermatogenesis.
They
appear
influence
how
stem
germ
cells
grow
divide
sperm
production
process.
Malfunctions
circRNA
activity
could
contribute
male
issues
stemming
abnormalities
In
current
review,
we
highlight
exciting
potential
key
players
fertility.
Язык: Английский
Dual-Omics Approach Unveils Novel Perspective on the Quality Control of Genetically Engineered Exosomes
Pharmaceutics,
Год журнала:
2024,
Номер
16(6), С. 824 - 824
Опубликована: Июнь 18, 2024
Exosomes,
nanoscale
vesicles
derived
from
human
cells,
offer
great
promise
for
targeted
drug
delivery.
However,
their
inherent
diversity
and
genetic
modifications
present
challenges
in
terms
of
ensuring
quality
clinical
use.
To
explore
solutions,
we
employed
advanced
gene
fusion
transfection
techniques
293T
cells
to
generate
two
distinct
sets
genetically
engineered
samples.
We
used
dual-omics
analysis,
combining
transcriptomics
proteomics,
comprehensively
assess
exosome
by
comparing
with
controls.
Transcriptomic
profiling
showed
increased
levels
engineering
scaffolds
the
modified
groups,
confirming
success
manipulation.
Through
transcriptomic
identified
15
RNA
species,
including
2008
miRNAs
13,897
mRNAs,
loaded
onto
exosomes,
no
significant
differences
miRNA
or
mRNA
between
control
exosomes.
Proteomics
analysis
changes
introduced
through
over
1330
endogenous
exosome-associated
proteins,
indicating
complex
nature
Further
pathway
enrichment
a
small
subset
cellular
signaling
pathways,
aiding
our
understanding
potential
biological
impacts
on
recipient
cells.
Detection
100
cow
proteins
highlighted
effectiveness
LC-MS
identifying
contaminants.
Our
findings
establish
framework
products,
facilitating
translation
therapeutic
applications
nanomedicine.
Язык: Английский
Emerging role of gut microbiota extracellular vesicle in neurodegenerative disorders and insights on their therapeutic management
iMetaOmics.,
Год журнала:
2024,
Номер
1(2)
Опубликована: Окт. 25, 2024
Abstract
Targeting
gut
flora
to
lower
sickness
risk
is
a
growing
scientific
subject.
The
intricate
network
of
microorganisms
(gut
microbiota)
in
the
human
intestines
regulates
many
physiological
systems
and
may
be
important
for
general
health.
Recent
research
has
shown
dynamic
relationship
between
microbiota
central
nervous
system
(CNS).
Dysbiosis
key
establishing
progressing
diseases,
including
neurodegenerative
disorders.
Recently,
extracellular
vesicles
(GMEVs)
have
been
suggested
as
brain‐gut
communication
carriers.
Vesicle
components
contact
immune
receptors,
initiating
neuroinflammatory
responses
causing
diseases.
This
study
seeks
explain
how
its
cause
or
worsen
We
also
highlighted
recent
advances
our
understanding
these
GMEVs'
cargo's
routes,
which
could
used
drug
delivery
treatments.
examines
current
state
potential
therapeutic
effects
GMEVs
on
illnesses.
Язык: Английский