Transcriptional upregulation of HERV-env genes under simulated microgravity DOI Creative Commons
Seyedesomaye Jasemi, Elena Rita Simula, Antonella Pantaleo

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

Abstract Background Human Endogenous retroviruses (HERVs) constitute about 8% of the human genome. Overexpression HERVs has been detected in various inflammatory disorders like neuroinflammation and cancer. Interestingly, it reported that stress conditions facilitate expression. Space travel will expose astronauts to microgravity environments (a condition), which may result activation might influence pathogenic outcomes during after space flight. This study aimed elucidate transcriptional activity three HERV families (W, K, H) cytokines genes (IL-1, IL-6, TNF-α) different cell lines under (µg) compare them with results obtained normal gravity (ng; 1g). Results We evaluated expression (HERV-K env, HERV-W HERV-H env) cytokine neuroblastoma (SH-SY5Y), HEp-2, Caco-2 simulated (1g) conditions. In SH-SY5Y cells, level IL-1, HERV-K env was significantly increased when exposed short-term µg (3 6 h). The TNF-α remained unchanged throughout all time points. Additionally, IL-1 higher hours incubation compared 1g. There no statistically significant difference levels IL6, between 1g Moreover, HEp-2 TNF-α, following h, h) and, then decreased comparable those observed condition. Conclusions Taken together, dysregulation HERV patterns these dysregulations varied demands further investigation for health protection space.

Язык: Английский

Transcriptional Upregulation of HERV-env Genes Under Simulated Microgravity DOI Creative Commons
Seyedesomaye Jasemi, Elena Rita Simula, Antonella Pantaleo

и другие.

Viruses, Год журнала: 2025, Номер 17(3), С. 306 - 306

Опубликована: Фев. 23, 2025

Human endogenous retroviruses (HERVs) constitute about 8% of the human genome. The overexpression HERVs has been detected in various inflammatory disorders like neuro-inflammation and cancer. Interestingly, it reported that stress conditions facilitate HERV expression. Space travel exposes astronauts to microgravity environments (a condition), which may result activation might influence pathogenic outcomes during after space flight. This study aimed elucidate transcriptional activity three families (W, K, H) cytokine genes (IL-1, IL-6, TNF-α) different cell lines under (μg) compare them with results obtained normal gravity (ng; 1g). We evaluated expression (HERV-K env, HERV-K gag, HERV-W HERV-H env) gene neuroblastoma (SH-SY5Y), HEp-2, Caco-2 simulated μg 1g conditions. In SH-SY5Y cells, level IL-1, env was significantly increased when exposed short-term (3 6 h). TNF-α remained unchanged throughout all time points. Additionally, IL-1 higher h incubation compared 1g. There no statistically significant difference levels IL6, between Moreover, HEp-2 TNF-α, following h, h) then decreased comparable those observed condition. Taken together, dysregulation patterns these dysregulations varied lines, demands further investigation for health protection space.

Язык: Английский

Процитировано

1
Treatment-resistant depression: molecular mechanisms and management DOI Creative Commons
Mayanja Kajumba, Angelina Kakooza‐Mwesige, Noeline Nakasujja

и другие.

Molecular Biomedicine, Год журнала: 2024, Номер 5(1)

Опубликована: Окт. 17, 2024

Due to the heterogeneous nature of depression, underlying etiological mechanisms greatly differ among individuals, and there are no known subtype-specific biomarkers serve as precise targets for therapeutic efficacy. The extensive research efforts over past decades have not yielded much success, currently used first-line conventional antidepressants still ineffective close 66% patients. Most clinicians use trial-and-error treatment approaches, which seem beneficial only a fraction patients, with some eventually developing resistance. Here, we review evidence from both preclinical clinical studies on pathogenesis depression antidepressant response. We also discuss efficacy pharmacological non-pharmacological well novel emerging therapies. reveals that in response, specific, but rather involve an interplay between various neurotransmitter systems, inflammatory mediators, stress, HPA axis dysregulation, genetics, other psycho-neurophysiological factors. None current hypotheses sufficiently accounts interactional involved its etiology could partly explain limited success discovering efficacious treatment. Effective management treatment-resistant (TRD) requires targeting several mechanisms, using and/or personalized modalities, could, example, include multi-target pharmacotherapies augmentation psychotherapy approaches. Future guided by interaction provide more insights into potential etiologies TRD, precision biomarker targets, modalities.

Язык: Английский

Процитировано

4
An integrated approach for the accurate detection of HERV-K HML-2 transcription and protein synthesis DOI Creative Commons
Charles Gleason, Sandra Terry, Matthew M. Hernandez

и другие.

Nucleic Acids Research, Год журнала: 2025, Номер 53(2)

Опубликована: Янв. 11, 2025

Abstract Human endogenous retroviruses (HERVs) occupy a large portion of the human genome. Most HERVs are transcriptionally silent, but they can be reactivated during pathological states such as viral infection and certain cancers. The HERV-K HML-2 clade includes elements that recently integrated have in germ line often contain intact open reading frames possibly support peptide protein expression. Understanding HERV–K-host interactions their potential biomarkers is problematic due to high similarity among different elements. Previously, we described long-read single molecule real-time sequencing (PacBio) strategy analyze RNA expression profiles cell types. However, identifying proteins accurately difficult without robust reliable methods reagents. Here present new approach characterize (a) being translated (b) produce enough detected identified by mass spectrometry. Our data reveal profiling alone cannot predict which responsible for production, observe several differences between highest expressed RNAs predominant source synthesis. These studies represent an important advance toward untangling complexity interactions.

Язык: Английский

Процитировано

0
Recapitulation and reversal of neuropsychiatric phenotypes in a mouse model of human endogenous retrovirus type W expression DOI Creative Commons
Felisa Herrero, Celine Heeb,

Michelle G. Meier

и другие.

Molecular Psychiatry, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Abstract Human endogenous retroviruses (HERVs) are inherited genetic elements derived from exogenous retroviral infections occurring throughout evolution. Accumulating evidence implicates increased expression of HERV type W envelope (HERV-W ENV) in psychiatric and neurodevelopmental disorders. To gain more mechanistic insights into the neurobiological disease pathways affected by HERV-W ENV expression, we took advantage a mouse model that recapitulates human-specific protein. Behavioral cognitive phenotyping transgenic (TG) mice expressing wild-type (WT) controls showed this caused deficits numerous functional domains, including repetitive behavior, social object recognition memory, sensorimotor gating. Genome-wide RNA sequencing hippocampal tissue demonstrated led to transcriptomic alterations highly relevant for disorders, functions, synaptic development. Differential gene TG encompassed downregulation several genes associated with schizophrenia autism spectrum disorder, Setd1a , Cacna1g Ank3 Shank3 as well histone methyltransferase belong Set1-like H3 lysine 4 (H3K4) family ( Kmt2a Kmt2b Kmt2d ). Concomitant latter, displayed enzymatic activity lysine-specific demethylase-1 (LSD1), H3K4 mono-methylation, decreased di- tri-methylation hippocampus. Importantly, pharmacological inhibition LSD1 through oral ORY-1001 treatment normalized abnormal methylation rescued behavioral mice. In conclusion, our study suggests has capacity disrupt various functions alter brain transcriptome manner is Moreover, identified epigenetic may offer avenues interventions against induced HERW-W expression.

Язык: Английский

Процитировано

0
Unraveling the Interaction between Inflammation and the Cardiometabolic Index in Older Men: A Pilot Study DOI Open Access
Rafael L. Carvalho, Tábatta Renata Pereira de Brito, Jônatas Bussador do Amaral

и другие.

Nutrients, Год журнала: 2024, Номер 16(15), С. 2529 - 2529

Опубликована: Авг. 2, 2024

Both cardiometabolic and chronic inflammatory diseases pose a significant challenge to global public health, particularly among older adults. Here, we investigated the interplay between systemic status index (CMI) in men with adequate weight or obesity. In this observational cross-sectional study, (71.79 ± 7.35 years) were separated into groups normal (NW,

Язык: Английский

Процитировано

1
Transcriptional upregulation of HERV-env genes under simulated microgravity DOI Creative Commons
Seyedesomaye Jasemi, Elena Rita Simula, Antonella Pantaleo

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

Abstract Background Human Endogenous retroviruses (HERVs) constitute about 8% of the human genome. Overexpression HERVs has been detected in various inflammatory disorders like neuroinflammation and cancer. Interestingly, it reported that stress conditions facilitate expression. Space travel will expose astronauts to microgravity environments (a condition), which may result activation might influence pathogenic outcomes during after space flight. This study aimed elucidate transcriptional activity three HERV families (W, K, H) cytokines genes (IL-1, IL-6, TNF-α) different cell lines under (µg) compare them with results obtained normal gravity (ng; 1g). Results We evaluated expression (HERV-K env, HERV-W HERV-H env) cytokine neuroblastoma (SH-SY5Y), HEp-2, Caco-2 simulated (1g) conditions. In SH-SY5Y cells, level IL-1, HERV-K env was significantly increased when exposed short-term µg (3 6 h). The TNF-α remained unchanged throughout all time points. Additionally, IL-1 higher hours incubation compared 1g. There no statistically significant difference levels IL6, between 1g Moreover, HEp-2 TNF-α, following h, h) and, then decreased comparable those observed condition. Conclusions Taken together, dysregulation HERV patterns these dysregulations varied demands further investigation for health protection space.

Язык: Английский

Процитировано

0