BMC Musculoskeletal Disorders, Год журнала: 2024, Номер 25(1)

Опубликована: Май 6, 2024

Abstract Background With the increasing incidence of steroid-induced necrosis femoral head (SNFH), numerous scholars have investigated its pathogenesis. Current evidence suggests that imbalance between lipogenesis and osteoblast differentiation in bone marrow mesenchymal stem cells (BMSCs) is a key pathological feature SNFH. MicroRNAs (miRNAs) strong gene regulatory effects can influence direction cell differentiation. N6-methyladenosine (m6A) prevalent epigenetic modification involved diverse pathophysiological processes. However, knowledge how miRNAs regulate m6A-related factors affect BMSC limited. Objective We aimed to investigate role miR27a regulating expression YTHDF2 BMSCs. Methods compared miR27a, YTHDF2, total m6A mRNA levels SNFH-affected control CCK-8 TUNEL assays were used assess proliferation apoptosis. Western blotting qRT‒PCR measure osteogenic (ALP, RUNX2, OCN) lipogenic (PPARγ C/EBPα) markers. Alizarin Red Oil O staining quantify differentiation, respectively. was knocked down or overexpressed evaluate impact on relationship with YTHDF2. Bioinformatics analyses identified as differentially expressed SNFH (ROC analysis) revealed potential signaling pathways through GSEA. The silencing functions BMSCs assessed. Results downregulation upregulation observed knockdown/overexpression modulated expression, impacting decreased methylation promoted while exerted similar effects. GSEA suggested associated Conclusion regulates affecting promoting osteogenesis. This finding therapeutic target for

Язык: Английский