Evaluating the effects of c-phycocyanin from cyanobacterium Spirulina platensis as an anti-inflammatory agent on stimulated human chondrocyte cells

Applied Phycology, Год журнала: 2025, Номер unknown, С. 1 - 12

Опубликована: Янв. 15, 2025

Inflammation is a critical defence mechanism in higher animals but uncontrolled inflammation implicated many diseases and can lead to chronic conditions, posing significant challenges healthcare the global economy. Conventional therapies often come with limitations adverse effects, underscoring need for safer more effective alternatives. This study investigates efficacy of C-Phycocyanin (C-PC), pigment derived from Spirulina platensis cultivated under optimal light as an anti-inflammatory agent. research focused on its effects nitric oxide secretion, which supports body's immune system by killing pathogens, interleukin-1 beta (IL-1β) gene expression inflammatory indicator, phorbol 12-myristate 13-acetate (PMA)-stimulated human chondrocyte cells (C28/I2). The Methyl Thiazol Tetrazolium (MTT) assay was employed evaluate metabolic activity confirm non-toxicity C-PC. results demonstrated that C-PC maintained 99.32% cell viability C28/I2 vitro model after seven days. Notably, treatment prevented death promoted proliferation, increasing number viable 12.34% during same period. Additionally, it significantly reduced IL-1β secretion 70.24% 91.25%, respectively, effectively reducing levels those observed unstimulated conditions. Scanning electron microscopy (SEM) crystal violet staining showed restored normal morphology protected inflammation-induced changes.

Язык: Английский

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Application and progress of smart hydrogel microspheres for regulating oxidative stress in osteoarthritis

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160620 - 160620

Опубликована: Фев. 1, 2025

Язык: Английский

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Integrating network pharmacology, molecular docking, and experimental verification to demonstrate that Jiawei Duhuo Parasitic Decoction treated osteoarthritis by inhibiting PTGS2 expression

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2025, Номер unknown, С. 100601 - 100601

Опубликована: Март 1, 2025

Язык: Английский

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Cuproptosis and its potential role in musculoskeletal disease

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Апрель 11, 2025

Cuproptosis, a recently identified form of copper-dependent cell death, arises from intracellular copper dyshomeostasis. As an essential trace element, plays critical role in bioenergetic metabolism, redox regulation, and synaptic transmission. However, excessive exerts cytotoxic effects through multiple pathways, including increased reactive oxygen species (ROS) production, apoptotic cascade activation, necrotic membrane rupture, inflammatory responses, mitochondrial dysfunction. Distinct other death mechanisms, cuproptosis is characterized by ion binding to acetylated respiratory chain proteins, leading pathogenic protein aggregation, iron-sulfur cluster depletion, cellular collapse. Emerging evidence underscores aberrant accumulation resultant proteotoxic stress as pivotal contributors the pathogenesis musculoskeletal pathologies, osteoporosis, osteoarthritis, sarcopenia, osteosarcoma, intervertebral disc degeneration, spinal cord injury, biofilm-associated orthopedic infections. Understanding spatiotemporal regulation may provide novel opportunities for advancing diagnostic therapeutic approaches medicine. This review synthesizes current insights into molecular mechanisms cuproptosis, its diseases, potential biomarker-driven interventions.

Язык: Английский

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Identification of biomarkers related to autophagy in osteoarthritis via bioinformatics analysis and experimental validation (Preprint)

Опубликована: Ноя. 7, 2024

Osteoarthritis(OA) is a degenerative disease with few effective early interventions and therapeutic options, autophagy thought to play significant role in progression of OA. We used bioinformatics analysis experimental validation identify biomarkers related OA the aim targets. The series matrix file GSE82107 was downloaded from Gene Expression Omnibus (GEO) public databases. data were processed differentially expressed genes (DEGs) identified via R 4.2.2 software. related-DEGs subsequently defined as DEGs that overlapped autophagy-related selected on basis Venn diagram. screened Ontology (GO) analysis, Kyoto Encyclopedia Genes Genomes (KEGG) enrichment protein-protein interaction (PPI) immune cell infiltration analysis. Based constructed PPI network, hub CytoHubba plugin. Moreover, confirmed receiver operating characteristic (ROC) curve GSE129147 GSE169077 datasets. In addition, we collected synovial tissue knees ten patients: five patients who underwent total knee arthroplasty young arthroscopic surgery for anterior cruciate ligament rupture. samples subjected RNA extraction followed by real-time quantitative RT-PCR.Finally, verified expression HUB gene calculating corresponding mRNA levels. Five namely, BCL2L1, CTSD, ATG3, GABARAPL2 CTSB related-DEGs. ROC revealed almost all had good diagnostic value, but Q-PCR results clinical GABARAPL2, upregulated (p < 0.05). relative expressions CTSD ATG3 not significantly different between group control > This study OA, including CTSB. These are promising targets treatment.

Язык: Английский

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Evaluation of the Healing Effect of Kappa-Carrageenan Combined with Dental Pulp Stem Cell-Derived Exosomes on Induced Knee Osteoarthritis in Male Rats

Опубликована: Янв. 1, 2024

Язык: Английский

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