Integrative Transcriptomics and Proteomics Analysis Reveals THRSP’s Role in Lipid Metabolism DOI Open Access
Yujie Li, Ke Xu,

Ao Zhou

и другие.

Genes, Год журнала: 2024, Номер 15(12), С. 1562 - 1562

Опубликована: Ноя. 30, 2024

Background/Objectives: Abnormalities in lipid metabolism and endoplasmic reticulum (ER) stress are strongly associated with the development of a multitude pathological conditions, including nonalcoholic fatty liver disease (NAFLD), diabetes mellitus, obesity. Previous studies have indicated potential connection between thyroid hormone responsive (THRSP) that ER may participate synthesis key regulators adipogenesis. However, specific mechanisms remain to be investigated. Methods: In this study, we explored roles THRSP by interfering gene expression mouse mesenchymal stem cells, comparing effects on adipogenesis control interfered groups, combining transcriptomic proteomic analysis. Results: Our results showed number droplets was significantly reduced after THRSP, levels adipogenesis, such as LPL, FABP4, PLIN1, CIDEC, were downregulated. Both differential genes (proteins) enriched processes lipolytic regulation, stress, cholesterol metabolism, sphingolipid PPAR signaling pathway, glycerophospholipid metabolism. The marker gene, ATF6, most downregulated transcription factor. addition, RT-qPCR validation pathway SCD1; droplet generation LIPE, DGAT1, AGPAT2; ATF6 Conclusions: These suggest is involved regulating which closely related Interfering helpful ameliorating occurrence diseases abnormalities

Язык: Английский

Modulating lipid droplet dynamics in neurodegeneration: an emerging area of molecular pharmacology DOI

RS Verma,

Prateek Sharma, Veerta Sharma

и другие.

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Март 3, 2025

Язык: Английский

Процитировано

0
Integrative Transcriptomics and Proteomics Analysis Reveals THRSP’s Role in Lipid Metabolism DOI Open Access
Yujie Li, Ke Xu,

Ao Zhou

и другие.

Genes, Год журнала: 2024, Номер 15(12), С. 1562 - 1562

Опубликована: Ноя. 30, 2024

Background/Objectives: Abnormalities in lipid metabolism and endoplasmic reticulum (ER) stress are strongly associated with the development of a multitude pathological conditions, including nonalcoholic fatty liver disease (NAFLD), diabetes mellitus, obesity. Previous studies have indicated potential connection between thyroid hormone responsive (THRSP) that ER may participate synthesis key regulators adipogenesis. However, specific mechanisms remain to be investigated. Methods: In this study, we explored roles THRSP by interfering gene expression mouse mesenchymal stem cells, comparing effects on adipogenesis control interfered groups, combining transcriptomic proteomic analysis. Results: Our results showed number droplets was significantly reduced after THRSP, levels adipogenesis, such as LPL, FABP4, PLIN1, CIDEC, were downregulated. Both differential genes (proteins) enriched processes lipolytic regulation, stress, cholesterol metabolism, sphingolipid PPAR signaling pathway, glycerophospholipid metabolism. The marker gene, ATF6, most downregulated transcription factor. addition, RT-qPCR validation pathway SCD1; droplet generation LIPE, DGAT1, AGPAT2; ATF6 Conclusions: These suggest is involved regulating which closely related Interfering helpful ameliorating occurrence diseases abnormalities

Язык: Английский

Процитировано

0