Quality and composition control of complex TCM preparations through a novel “Herbs-in vivo Compounds-Targets-Pathways” network methodology: the case of Lianhuaqingwen capsules DOI Creative Commons

Simian Chen,

Hui Qi,

Chunyan Zhu

и другие.

Pharmacological Research, Год журнала: 2025, Номер unknown, С. 107650 - 107650

Опубликована: Фев. 1, 2025

Lianhuaqingwen (LHQW) capsules, a Chinese patent medicine, consist of 13 herbal ingredients and are widely used for respiratory diseases. However, the complex composition LHQW poses challenges in assessing its quality consistency. In this study, comprehensive network was constructed by integrating Digital RNA with pertUrbation Genes (DRUG)-seq, sequencing, pharmacodynamic data. This enables rapid systematic screening compounds that exhibit high-exposure vivo significant activity potential, serving as potential control markers. details, DRUG-seq employed to evaluate gene expression alterations peripheral blood mononuclear cells derived from healthy volunteers. Ultrahigh-performance liquid chromatography coupled high-resolution mass spectrometry (UPLC-HRMS) analyze components rats, identifying total 505 compounds. Additionally, absorption, distribution, metabolism, excretion (ADME) profiles were plotted 27 primary LHQW. And an HPLC-MS/MS method quantified 46 LHQW, 15 them identified markers high exposure levels. These exhibited inhibitory effects on lipopolysaccharide (LPS)-induced pneumonia mice, mechanisms predicted RNA-seq verified RT-qPCR. summary, study successfully "Herbs- Compounds-targets-pathways" network, offering novel insights into establishing foundation enhancing measures.

Язык: Английский

Quality and composition control of complex TCM preparations through a novel “Herbs-in vivo Compounds-Targets-Pathways” network methodology: the case of Lianhuaqingwen capsules DOI Creative Commons

Simian Chen,

Hui Qi,

Chunyan Zhu

и другие.

Pharmacological Research, Год журнала: 2025, Номер unknown, С. 107650 - 107650

Опубликована: Фев. 1, 2025

Lianhuaqingwen (LHQW) capsules, a Chinese patent medicine, consist of 13 herbal ingredients and are widely used for respiratory diseases. However, the complex composition LHQW poses challenges in assessing its quality consistency. In this study, comprehensive network was constructed by integrating Digital RNA with pertUrbation Genes (DRUG)-seq, sequencing, pharmacodynamic data. This enables rapid systematic screening compounds that exhibit high-exposure vivo significant activity potential, serving as potential control markers. details, DRUG-seq employed to evaluate gene expression alterations peripheral blood mononuclear cells derived from healthy volunteers. Ultrahigh-performance liquid chromatography coupled high-resolution mass spectrometry (UPLC-HRMS) analyze components rats, identifying total 505 compounds. Additionally, absorption, distribution, metabolism, excretion (ADME) profiles were plotted 27 primary LHQW. And an HPLC-MS/MS method quantified 46 LHQW, 15 them identified markers high exposure levels. These exhibited inhibitory effects on lipopolysaccharide (LPS)-induced pneumonia mice, mechanisms predicted RNA-seq verified RT-qPCR. summary, study successfully "Herbs- Compounds-targets-pathways" network, offering novel insights into establishing foundation enhancing measures.

Язык: Английский

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