LncRNA SNHG12 in extracellular vesicles derived from carcinoma-associated fibroblasts promotes cisplatin resistance in non-small cell lung cancer cells DOI Open Access
Deli Tan, Gang Li, Peng Zhang

и другие.

Bioengineered, Год журнала: 2022, Номер 13(1), С. 1838 - 1857

Опубликована: Янв. 1, 2022

Non-small-cell lung cancer (NSCLC) is defined as the most universally diagnosed class of cancer. Cisplatin (DDP) an effective drug for NSCLC, but tumors are prone to resistance. The current study set out evaluate regulatory effect long non-coding RNA (lncRNA) small nucleolar host gene 12 (SNHG12) in extracellular vesicles (EVs) derived from carcinoma-associated fibroblasts (CAFs) on DDP resistance NSCLC cells. Firstly, cells were treated with EVs, followed by detection cell activity, IC50 values, proliferation and apoptosis, Cy3-SNHG12. We observed that CAFs-EVs promoted values inhibited apoptosis. In addition, we learned lncRNA SNHG12 carried into facilitated Furthermore, ELAV like binding protein 1 (HuR/ELAVL1) X-linked inhibitor apoptosis (XIAP) was verified stability XIAP also transcription XIAP, while silencing HuR could partially-reverse this promoting effect. Further joint experimentation showed partially Additionally, tumor growth positive rate Ki67 detected, which CAFs-oe-EVs Ki67, well levels SNHG12, HuR, vivo. Collectively, our findings indicated thus augmenting

Язык: Английский

N6-methyladenosine methyltransferases: functions, regulation, and clinical potential DOI Creative Commons
Wei Huang,

Tian-Qi Chen,

Ke Fang

и другие.

Journal of Hematology & Oncology, Год журнала: 2021, Номер 14(1)

Опубликована: Июль 27, 2021

Abstract N6-methyladenosine (m6A) has emerged as an abundant modification throughout the transcriptome with widespread functions in protein-coding and noncoding RNAs. It affects fates of modified RNAs, including their stability, splicing, and/or translation, thus plays important roles posttranscriptional regulation. To date, m6A methyltransferases have been reported to execute deposition on distinct RNAs by own or forming different complexes additional partner proteins. In this review, we summarize function these regulating key genes pathways cancer biology. We also highlight progress use mediating therapy resistance, chemotherapy, targeted therapy, immunotherapy radiotherapy. Finally, discuss current approaches clinical potential methyltransferase-targeting strategies.

Язык: Английский

Процитировано

182
The plasticity of mRNA translation during cancer progression and therapy resistance DOI
Lucilla Fabbri, Alina Chakraborty, Caroline Robert

и другие.

Nature reviews. Cancer, Год журнала: 2021, Номер 21(9), С. 558 - 577

Опубликована: Авг. 2, 2021

Язык: Английский

Процитировано

175
Circular RNAs: characteristics, biogenesis, mechanisms and functions in liver cancer DOI Creative Commons
Hao Shen, Boqiang Liu, Junjie Xu

и другие.

Journal of Hematology & Oncology, Год журнала: 2021, Номер 14(1)

Опубликована: Авг. 30, 2021

Abstract Background Hepatocellular carcinoma (HCC) is one of the most common malignancies globally. Despite aggressive and multimodal treatment regimens, overall survival HCC patients remains poor. Main Circular RNAs (circRNAs) are noncoding (ncRNAs) with covalently closed structures tissue- or organ-specific expression patterns in eukaryotes. They highly stable have important biological functions, including acting as microRNA sponges, protein scaffolds, transcription regulators, translation templates interacting RNA-binding protein. Recent advances indicated that circRNAs present abnormal tissues their dysregulation contributes to initiation progression. Furthermore, researchers revealed some might serve diagnostic biomarkers drug targets clinical settings. In this review, we systematically evaluate characteristics, biogenesis, mechanisms functions further discuss current shortcomings potential directions prospective studies on liver cancer-related circRNAs. Conclusion CircRNAs a novel class ncRNAs play significant role progression, but internal applications need investigation.

Язык: Английский

Процитировано

104
RNA-binding proteins and cancer metastasis DOI
Shengjie Wang,

Zelong Sun,

Zhe Lei

и другие.

Seminars in Cancer Biology, Год журнала: 2022, Номер 86, С. 748 - 768

Опубликована: Март 23, 2022

Язык: Английский

Процитировано

94
Protein synthesis control in cancer: selectivity and therapeutic targeting DOI Open Access
Joanna Kovalski, Duygu Kuzuoğlu‐Öztürk, Davide Ruggero

и другие.

The EMBO Journal, Год журнала: 2022, Номер 41(8)

Опубликована: Март 22, 2022

Язык: Английский

Процитировано

80
Alternative splicing and related RNA binding proteins in human health and disease DOI Creative Commons

Yining Tao,

Qi Zhang, Haoyu Wang

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Фев. 2, 2024

Abstract Alternative splicing (AS) serves as a pivotal mechanism in transcriptional regulation, engendering transcript diversity, and modifications protein structure functionality. Across varying tissues, developmental stages, or under specific conditions, AS gives rise to distinct splice isoforms. This implies that these isoforms possess unique temporal spatial roles, thereby associating with standard biological activities diseases. Among these, AS-related RNA-binding proteins (RBPs) play an instrumental role regulating alternative events. Under physiological the diversity of mediated by influences structure, function, interaction, localization proteins, participating differentiation development array tissues organs. pathological alterations are linked various diseases, particularly cancer. These changes can lead gene patterns, culminating loss For instance, cancer, abnormalities RBPs may result aberrant expression cancer-associated genes, promoting onset progression tumors. also associated numerous neurodegenerative diseases autoimmune Consequently, study across different holds significant value. review provides detailed account recent advancements tissue which aids deepening understanding complexity offers new insights methodologies for precision medicine.

Язык: Английский

Процитировано

71
Smartphone-assisted self-powered dual-mode biosensor designed on binary 3D DNA Walkers mediated CRISPR/Cas12a system DOI

Jinyue Shi,

Peiyuan Li, Yumei Huang

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 483, С. 149231 - 149231

Опубликована: Янв. 30, 2024

Язык: Английский

Процитировано

28
HPV E6/E7 promotes aerobic glycolysis in cervical cancer by regulating IGF2BP2 to stabilize m6A-MYC expression DOI Creative Commons
Chenchen Hu,

Tianyue Liu,

Chenying Han

и другие.

International Journal of Biological Sciences, Год журнала: 2021, Номер 18(2), С. 507 - 521

Опубликована: Дек. 15, 2021

Enhanced aerobic glycolysis constitutes an additional source of energy for tumor proliferation and metastasis. Human papillomavirus (HPV) infection is the main cause cervical cancer (CC); however, associated molecular mechanisms remain poorly defined, as does relationship between CC glycolysis. To investigate whether HPV 16/18 E6/E7 can enhance in CC, expression was knocked down SiHa HeLa cells using small interfering RNA (siRNA). Then, glucose uptake, lactate production, ATP levels, reactive oxygen species (ROS) content, extracellular acidification rate (ECAR) consumption (OCR) were evaluated. RNA-seq used to probe mechanism involved E6/E7-driven glycolysis, identified IGF2BP2 a target E6/E7. The regulatory effect confirmed by qRT-PCR, western blot, RIP assay. biological roles underlying how promote progression vitro vivo. tissue microarrays analyze CC. knockdown attenuated glycolytic capacity growth cells, while overexpression rescued this higher tissues than adjacent positively correlated with stage. Mechanistically, proteins promoted proliferation, metastasis regulating MYC mRNA m6A modifications through IGF2BP2. We found that methylation sites via activating established link promotion progression. Blocking E6/E7-related metabolic pathway represents potential strategy treatment

Язык: Английский

Процитировано

85
RNA-Binding Proteins Hold Key Roles in Function, Dysfunction, and Disease DOI Creative Commons

Sophia Kelaini,

Celine Chan,

Victoria A. Cornelius

и другие.

Biology, Год журнала: 2021, Номер 10(5), С. 366 - 366

Опубликована: Апрель 24, 2021

RNA-binding proteins (RBPs) are multi-faceted in the regulation of RNA or its splicing, localisation, stability, and translation. Amassing proof from many recent dedicated studies reinforces perception RBPs exerting control through differing expression levels, cellular localization post-transcriptional alterations. However, since is reliant on micro-environment events like stress response metabolism, their binding affinities resulting RNA-RBP networks may be affected. Therefore, any misregulation disruption features related homeostasis can lead to a number diseases that include diabetes, cardiovascular disease, other disorders such as cancer neurodegenerative diseases. As such, correct crucial good health effect exert loss function cause pathogenesis. In this review, we will discuss significance typical how disrupted disease.

Язык: Английский

Процитировано

71
HNRNPC downregulation inhibits IL‐6/STAT3‐mediated HCC metastasis by decreasing HIF1A expression DOI Creative Commons
Danfei Liu, Xiangyuan Luo, Meng Xie

и другие.

Cancer Science, Год журнала: 2022, Номер 113(10), С. 3347 - 3361

Опубликована: Июль 18, 2022

RNA-binding protein (RBP) dysregulation is functionally linked to several human diseases, including neurological disorders, cardiovascular disease, and cancer. Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a diverse family of RBPs involved in nucleic acid metabolism. A growing body studies has shown that the dysregulated hnRNPs play important roles tumorigenesis. Here, we found heterogeneous ribonucleoprotein C (C1/C2) (HNRNPC) had good performance distinguishing between hepatocellular carcinoma (HCC) normal liver tissues through bioinformatics analysis. Further investigation revealed HNRNPC was significantly correlated with multiple malignant characteristics HCC, tumor size, microvascular invasion, differentiation, TNM stage. Patients HCC positive expression exhibited decreased overall survival increased recurrence rate. downregulation inhibited invasion metastasis. The hypoxia inducible factor 1 subunit alpha (HIF1A) identified as molecular mechanism underlying downregulation-inhibited metastasis by RNA sequencing. Mechanistically, HIF1A destabilizing mRNA. overexpression rescued decrease invasiveness induced downregulation. Additionally, interleukin (IL)-6/STAT3 signaling upregulated cells, knockdown IL-6/STAT3-enhanced Furthermore, anti-IL-6 antibody siltuximab IL-6-mediated In summary, our research clinical value, functional role, showed potential biomarker for diagnosis, prognosis, further therapeutic targets HCC.

Язык: Английский

Процитировано

41