Microbial production of α-amylase from agro-waste: An approach towards biorefinery and bio-economy
Energy Nexus,
Год журнала:
2024,
Номер
14, С. 100293 - 100293
Опубликована: Апрель 16, 2024
Economic
utilization
of
waste
in
the
generation
value-added
products
is
primary
prerequisite
a
circular
economy.
Agro-waste
one
that
enormous
on
hand
and
rich
nutrients
bioproducts
other
hand.
Microbial
fermentation
an
easy
technology
can
work
following
nature
substrate.
Development
such
process
very
much
possible
however
it
faces
initial
challenge
diversity
substrates,
microorganisms,
enzymes,
bioproducts.
It
would
be
thus
ideal
to
make
axis
product
then
optimize
around
this
axis.
Here
we
explore
potential
widely
industrially
used
enzyme
α-amylase
as
terminal
The
starch-rich
agro-waste
like
cereal
waste.
connection
between
these
two
terminals
producing
microorganism.
A
lot
e.g.
nutraceuticals,
biofuels,
fertilizers,
nanoparticles,
etc.
are
This
review
explores
suitability
serve
We
discuss
has
for
production,
industrial
applicability
α-amylase,
microorganisms
known
bioengineered
produce
optimization
production
process.
Язык: Английский
Exploring the termite gut as a hub of industrially important microbes and enzymes for biofuel production
Biomass and Bioenergy,
Год журнала:
2025,
Номер
199, С. 107899 - 107899
Опубликована: Апрель 16, 2025
Язык: Английский
Promoters and Synthetic Promoters in Trichoderma reesei
Methods in molecular biology,
Год журнала:
2024,
Номер
unknown, С. 47 - 68
Опубликована: Янв. 1, 2024
Язык: Английский
Unveiling the secretome of Penicillium fuscoglaucum JAM-1 for efficient dual substrate degradation and waste valorization
Biomass Conversion and Biorefinery,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 15, 2024
Abstract
In
the
pursuit
of
cost-effective
and
superior
enzymes
crucial
for
efficient
hydrolysis
diverse
lignocellulosic
biomasses,
filamentous
fungi
have
emerged
as
key
candidates
bioprospecting
endeavors.
our
exploration
potent
biomass
degraders,
we
identified
a
strain
Penicillium
fuscoglaucum
JAM-1,
showcasing
multipurpose
hydrolase
capabilities
in
its
secretome.
During
fermentation,
P.
JAM-1
effectively
utilized
rapeseed
cake
(RSC),
resulting
improved
enzymatic
activities,
including
xylanase
(612
U/gds),
β-glucosidase
(264
endoglucanase
(102
FPase
(21.3
exo-polygalacturonase
(49.17
compared
to
pine
sawdust
(PSD).
Secretome
profiling
revealed
protein
abundance
totaling
435
120
proteins
during
RSC
PSD
utilization,
respectively.
The
major
component
carbohydrate-active
(CAZymes)
consists
cellulose-degrading
proteins,
endoglucanases
(GH5,
GH7),
β-glucosidases
(GH1,
GH3,
GH17),
cellobiohydrolases
(GH6,
GH7).
Correspondingly,
hemicellulose-degrading
were
present,
encompassing
endo-1,4-xylanase
(GH10),
α/β-galactosidase
(GH27,
GH35,
GH36),
α/β-mannosidases
(GH38,
GH2,
GH47,
GH5),
α-
l
-arabinofuranosidase
(GH43,
GH62,
GH51,
GH54)
auxiliary
activities
enzymes,
such
AA9
(formerly
known
GH61)
lytic
polysaccharide
monooxygenase
(LPMO).
Upon
application
fruit
waste,
crude
enzyme
demonstrated
higher
saccharification
potential
commercial
cellulase
(Cellic
CTec2).
Specifically,
yielded
565
mg/g
reducing
sugar
within
72
h,
outperforming
Cellic
CTec2,
which
352
under
identical
conditions.
A
comprehensive
comparative
analysis
workings,
secretome
underscores
producer,
boost
lignocellulose
biodegradation.
These
findings
highlight
practical
applications
various
industrial
processes,
suggesting
role
valuable
candidate
further
exploitation
biotechnological
applications.
Graphical
abstract
Язык: Английский
Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production
BMC Biotechnology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 18, 2024
The
eco-friendly
transformation
of
agro-industrial
wastes
through
microbial
bioconversion
could
address
sustainability
challenges
in
line
with
the
United
Nations'
Sustainable
Development
Goals.
bulk
waste
consists
lignocellulosic
materials
fermentable
sugars,
predominantly
cellulose
and
hemicellulose.
A
number
pretreatment
options
have
been
employed
for
material
saccharification
toward
successful
fermentation
into
second-generation
bioethanol.
Biological
and/or
enzymatic
substrates
offers
sustainable
bioethanol
production
opportunities
that
may
also
contribute
to
management
without
affecting
food
security.
In
this
study,
we
isolated
a
promising
filamentous
bacterium
from
guts
cockroaches
commendable
cellulolytic
activity.
matrices
sequential
statistics,
one-factor-at-a-time
(OFAT)
significant
variable
screening
by
Placket-Burman
design
(PBD)
Box‒Behnken
surface
methodology
(BBD-RSM),
were
major
medium
modeling
optimization
solid-state
fermentation.
optimized
solutions
used
saccharify
lignocellulose
real
time,
kinetics
reducing
sugar
accumulation
subsequently
evaluated
determine
maximum
concentration
sugars
extracted
lignocellulose.
hydrolysate
highest
was
subjected
Saccharomyces
cerevisiae,
Klyuveromyces
marxianus
mixture
both,
after
which
ethanol
yield,
efficiency
determined.
Sequential
statistics
revealed
rice
husk
powder,
corn
cob
peptone
inoculum
volume
variables
bioprocess
at
59.8%
(w/w)
17.8%
38.8%
(v/w;
109
cfu/mL)
volume,
5.3%
peptone.
These
conditions
mediated
xylanolytic
activities
219.93
±
18.64
FPU/mL
333.44
22.74
U/mL,
respectively.
under
two
peaks
between
16
32
h
another
56
64
h.
Although
K.
had
significantly
greater
than
S.
50:50
(%
v/v)
both
yeasts
led
88.32%
55.56
0.19
g/L
crude
bioethanol,
suggesting
inexpensive,
be
obtained
renewable
energy
sources.
Язык: Английский