PARP‐1 Inhibition Increases Oxidative Stress in Ets‐1‐Expressing MDA‐MB‐231 Breast Cancer Cells

Cancer Reports, Год журнала: 2025, Номер 8(1)

Опубликована: Янв. 1, 2025

The Ets-1 transcription factor plays a primordial role in regulating the expression of numerous genes implicated cancer progression. In previous study, we revealed that poly(ADP-ribose) polymerase-1 (PARP-1) inhibition by PJ-34 results level increase cells, which is related with cell death Ets-1-expressing cells. mechanism antitumor effect PARP-1 was investigated MDA-MB-231 breast We tested effects four PARP inhibitors (PARPi) (PJ-34, Veliparib, Olaparib, and Rucaparib). first demonstrated PARPi reduced cells growth through G2/M cycle arrest. Next, evaluated on oxidative DNA damage Ets-1-overexpressing Ets-1-non-expressing showed led only to accumulate it, triggers response as panel damage-related proteins. Importantly, increased reactive oxygen species (ROS), this accompanied upregulation p47phox expression, subunit NAPDH oxidase (NOX). These preliminary findings correlate PARPi-induced damage/oxidative stress

Язык: Английский

PARylation-mediated post-transcriptional modifications in cancer immunity and immunotherapy

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 11, 2025

Poly-ADP-ribosylation (PARylation) is a post-translational modification in which ADP-ribose added to substrate proteins. PARylation mediated by superfamily of ADP-ribosyl transferases known as PARPs and influences wide range cellular functions, including genome integrity maintenance, the regulation proliferation differentiation. We others have recently reported that SH3 domain-binding protein 2 (3BP2) plays role bone metabolism, immune system regulation, cytokine production. Additionally, has gained attention target for cancer treatment. In this review, we provide an overview PARylation, its involvement several signaling pathways related immunity, potential combination therapies with PARP inhibitors checkpoint inhibitors.

Язык: Английский

The complex universe of inactive PARP1

Trends in Genetics, Год журнала: 2024, Номер unknown

Опубликована: Сен. 1, 2024

Язык: Английский