Detection of Cancer Stem Cells from Patient Samples
Cells,
Год журнала:
2025,
Номер
14(2), С. 148 - 148
Опубликована: Янв. 20, 2025
The
existence
of
cancer
stem
cells
(CSCs)
in
various
tumors
has
become
increasingly
clear
addition
to
their
prominent
role
therapy
resistance,
metastasis,
and
recurrence.
For
early
diagnosis,
disease
progression
monitoring,
targeting,
there
is
a
high
demand
for
clinical-grade
methods
quantitative
measurement
CSCs
from
patient
samples.
Despite
years
active
research,
standard
not
yet
reached
clinical
settings,
especially
the
case
solid
tumors.
This
because
detecting
this
plastic
heterogeneous
population
straightforward.
review
summarizes
techniques,
highlighting
benefits
limitations
In
addition,
designed
detect
based
on
secreted
niche-associated
signaling
factors
are
reviewed.
Spatial
single-cell
analyzing
tumor
tissues
noninvasive
techniques
such
as
liquid
biopsy
vivo
imaging
discussed.
Additionally,
recently
established
laboratories,
preclinical
studies,
assays
covered.
Finally,
we
discuss
characteristics
an
ideal
method
look
toward
future.
Язык: Английский
Cancer-associated fibroblasts in hepatocellular carcinoma: heterogeneity, mechanisms and therapeutic targets
Hepatology International,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
Язык: Английский
Quantifying and interpreting biologically meaningful spatial signatures within tumor microenvironments
npj Precision Oncology,
Год журнала:
2025,
Номер
9(1)
Опубликована: Март 11, 2025
The
tumor
microenvironment
(TME)
plays
a
crucial
role
in
orchestrating
cell
behavior
and
cancer
progression.
Recent
advances
spatial
profiling
technologies
have
uncovered
novel
signatures,
including
univariate
distribution
patterns,
bivariate
relationships,
higher-order
structures.
These
signatures
the
potential
to
revolutionize
mechanism
treatment.
In
this
review,
we
summarize
current
state
of
signature
research,
highlighting
computational
methods
uncover
spatially
relevant
biological
significance.
We
discuss
impact
these
on
fundamental
biology
translational
address
challenges
future
research
directions.
Язык: Английский
Clonorchis sinensis-infected hepatocellular carcinoma exhibits distinct tumor microenvironment and molecular features
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Март 17, 2025
Clonorchis
sinensis
(Cs)-infected
hepatocellular
carcinoma
(HCC)
patients
have
a
poorer
prognosis
than
non-Cs-infected
HCCs.
However,
the
molecular
mechanisms
of
Cs-infected
HCC
remain
unclear.
To
address
this,
this
study
aims
to
uncover
tumor
microenvironment
and
features
that
may
contribute
these
poor
outcomes.
The
research
involved
bulk
RNA
sequencing
paired
adjacent
tissue
samples
from
10
Cs
+
-
patients.
Differentially
expressed
genes
were
identified,
followed
by
enrichment
analyses
reveal
functional
changes.
Survival
analysis
top
up-
down-regulated
in
tumors
was
performed
using
TCGA
database.
Additionally,
clinical
data
1,461
retrospectively
analyzed
assess
impact
infection
on
microvascular
invasion
metastasis
rates.
In
vitro
assays
also
conducted
excretory/secretory
products
(CsESPs)
examine
their
effect
cells
HUVECs.
We
identified
785
up-regulated
675
compared
tumors,
enriched
pathways
related
extracellular
matrix
remodeling
immunosuppression.
revealed
are
associated
with
prognosis.
Clinical
showed
increased
vitro,
CsESPs
enhanced
migration
promoted
tube
formation
human
umbilical
vein
endothelial
cells.
This
provides
novel
insights
into
landscape
underscores
infection's
role
enhancing
migration,
angiogenesis.
findings
understanding
parasitic
infections
cancer
progression
suggest
potential
prognostic
markers
for
HCC.
Язык: Английский
Benign non-immune cells in tumor microenvironment
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 3, 2025
The
tumor
microenvironment
(TME)
is
a
highly
complex
and
continuous
evolving
ecosystem,
consisting
of
diverse
array
cellular
non-cellular
components.
Among
these,
benign
non-immune
cells,
including
cancer-associated
fibroblasts
(CAFs),
adipocytes,
endothelial
cells
(ECs),
pericytes
(PCs),
Schwann
(SCs)
others,
are
crucial
factors
for
development.
Benign
within
the
TME
interact
with
both
immune
cells.
These
interactions
contribute
to
progression
through
direct
contact
indirect
communication.
Numerous
studies
have
highlighted
role
that
exert
on
potential
tumor-promoting
mechanisms
via
multiple
signaling
pathways
factors.
However,
these
may
play
different
roles
across
cancer
types.
Therefore,
it
important
understand
based
heterogeneity.
A
deep
understanding
allows
us
develop
novel
therapies
by
targeting
In
this
review,
we
will
introduce
several
types
according
heterogeneity
their
in
TME.
Язык: Английский
CAF-derived miR-642a-3p supports migration, invasion, and EMT of hepatocellular carcinoma cells by targeting SERPINE1
PeerJ,
Год журнала:
2024,
Номер
12, С. e18428 - e18428
Опубликована: Ноя. 11, 2024
Background
Cancer-associated
fibroblasts
(CAFs)
and
hepatocellular
carcinoma
(HCC)
cells
interact
to
promote
HCC
progression,
but
the
underlying
mechanisms
remain
unclear.
Serpin
family
E
member
1
(SERPINE1)
has
conflicting
roles
in
HCC,
microRNAs
(miRNAs)
are
known
regulate
tumor
progression
through
intercellular
communication.
Therefore,
we
investigated
potential
involvement
of
miRNA/SERPINE1
axis
crosstalk
between
CAFs
cells.
Methods
In
this
study,
candidate
miRNAs
targeting
SERPINE1
3′
UTR
were
predicted
using
multiple
miRNA
databases.
The
mRNA
expression
Huh7
was
assessed
after
co-culture
with
RT-qPCR.
cell
proliferation
invasion
detected
siRNA.
functions
CAF-derived
miR-642a-3p/SERPINE1
examined
CCK-8,
wound
healing,
transwell
assays,
western
blot,
dual-luciferase
reporter
assays.
Moreover,
a
orthotopic
xenograft
model
used
investigate
contribution
miR-642a-3p
knockdown
HCC.
Results
decreased,
while
increased
co-cultured
CAFs.
enhanced
as
well
expression.
overexpression
promoted
migration,
invasion,
epithelial-mesenchymal
transition
(EMT)
by
SERPINE1,
yielded
opposite
effect.
Rescue
experiments
confirmed
that
attenuated
inhibitory
effects
on
EMT
Importantly,
suppressed
growth
liver
tumors.
Conclusion
facilitated
cells,
suggesting
can
be
therapeutic
target
for
Язык: Английский
The metabolic crosstalk of cancer-associated fibroblasts and tumor cells: Recent advances and future perspectives
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer,
Год журнала:
2024,
Номер
unknown, С. 189190 - 189190
Опубликована: Сен. 1, 2024
Язык: Английский
Tumor exosomal RNPEP promotes lung metastasis of liver cancer via inducing cancer‐associated fibroblast activation
Cancer Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
Cancer-associated
fibroblasts
(CAFs)
are
essential
players
in
the
tumor
microenvironment
(TME)
due
to
their
roles
facilitating
progression
and
metastasis.
It
is
worth
noting
that
high-metastatic
hepatocellular
carcinoma
(HCC)
cell-derived
exosomes
have
exhibited
ability
transform
normal
into
CAFs,
which
further
fosters
lung
metastasis
of
low-metastatic
HCC
cells.
Yet,
mechanisms
underlying
this
exosome-induced
metastatic
niche
formation
poorly
explored.
In
study,
secreted
protein
arginyl
aminopeptidase
(RNPEP)
was
highly
expressed
plasma
patients
with
HCC.
addition,
cells
showed
augmented
RNPEP
expression
levels
exosomes.
These
induced
obvious
CAF-like
properties
human
fibroblast
cell
line
MRC-5,
as
evidenced
by
increased
CAF
marker
expression,
enhanced
migratory
ability.
More
strikingly,
secretions
from
exosome-educated
MRC-5
stemness
promoted
epithelial-mesenchymal
transition
(EMT)
MHCC-97L
cells,
a
line.
However,
knockdown
abated
changes
described
above.
Animal
studies
vivo
highlighted
pro-tumor
pro-metastatic
effects
exosomal
on
inducing
activation.
Furthermore,
tumor-derived
activation
NF-κB
signaling
critical
pathway
associated
Collectively,
these
results
provide
novel
insight
for
its
crosstalk
CAFs
during
Язык: Английский