An overview of nasal cartilage bioprinting: from bench to bedside

Journal of Biomaterials Science Polymer Edition, Год журнала: 2024, Номер 35(8), С. 1273 - 1320

Опубликована: Март 5, 2024

Nasal cartilage diseases and injuries are known as significant challenges in reconstructive medicine, affecting a substantial number of individuals worldwide. In recent years, the advent three-dimensional (3D) bioprinting has emerged promising approach for nasal reconstruction, offering potential breakthroughs field regenerative medicine. This paper provides an overview methods associated with 3D technologies procedure reconstructing tissue. The process entails generating digital model using biomedical imaging techniques computer-aided design to integrate both internal external scaffold features. Then, bioinks which consist biomaterials, cell types, bioactive chemicals, applied facilitate precise layer-by-layer tissue-engineered scaffolds. After undergoing vitro vivo experiments, this results development physiologically functional integrity advantages encompass ability customize design, enabling incorporation pore shape, size, porosity, well utilization patient-specific cells enhance compatibility. However, various should be considered, including optimization ensuring adequate viability differentiation, achieving seamless integration host tissue, navigating regulatory attention. Although numerous studies have demonstrated rebuilding such soft tissues, covers aspects bioprinted tissues provide insights future repair appropriate clinical use.

Язык: Английский

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Attenuation of osteoarthritis progression through intra-articular injection of a combination of synovial membrane-derived MSCs (SMMSCs), platelet-rich plasma (PRP) and conditioned medium (secretome)

Journal of Orthopaedic Surgery and Research, Год журнала: 2022, Номер 17(1)

Опубликована: Фев. 17, 2022

Osteoarthritis (OA) as a progressive destructive disease of articular cartilage is the most common joint characterized by reduction thickness, demolition surface and new bone formation. To overcome these problems, purpose current research was to evaluate compare in vivo effects synovial membrane-derived mesenchymal stem cell (SMMSCs), platelet-rich plasma (PRP) conditioned medium (secretome) on collagenase II-induced rat knee osteoarthritis (KOA) remedy.For first step, SMMSCs were isolated characterized. Also, secretome collected from culture. Furthermore, PRP collect heart venous blood. Second, two injection II with an interval 3 days performed intra-articular space induce osteoarthritis. Two weeks later, animals randomly divided into 6 groups. Control group without treatment, positive group: taken sodium hyaluronate (0.1 ml), treatment groups of; 1: (5 × 106), 2: 106)/secretome (50 µl), 3: 106)/PRP 4: 106)/ µl)/ µl). Three months rats killed following assessments executed: radiography, histopathology, immunohistochemistry.Our findings represented that combination SMMSCs/secretome/PRP had considerable effect glycosaminoglycans (GAGs) collagen contents, preservation, compared other In addition, showed lowest expression mmp3, while SOX9 highest comparison SMMSCs-injected demonstrated better results control groups.Injecting resulted efficacy terms width, continuity integrity, sub-chondral ECM constituents such II. Indeed, transplantation this could be considered preliminary therapy for clinical trial study future.

Язык: Английский

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Clinical Study on the Treatment of Type 2 Diabetes with Bone Marrow Platelet-Rich Plasma (BMPRP)

Journal of Stem Cell Therapy and Transplantation, Год журнала: 2025, Номер 9(2), С. 027 - 030

Опубликована: Март 28, 2025

Objective: To retrospectively analyze the effects of (bone marrow platelet-rich plasma BMPRP) precise pancreatic infusion therapy versus conventional treatment. Methods: Bone was collected from iliac crest anterior superior spine, and separated by centrifugation. BMPRP infused into pancreas under ultrasound guidance. It compared with hypoglycemic drugs insulin therapy. Results: In treatment group 32 cases, fasting blood sugar hemoglobin A1c were significantly lower than pre-treatment levels, while C-peptide level did not change significantly. The dose reduced. 28 sugar, A1c, levels after continuous for one year, Conclusion: can improve function, reduce resistance, required dosage.

Язык: Английский

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Effects of Stem Cells on Rat Embryo Development in Hypoxy Embryo Culture

Bratislavské lekárske listy/Bratislava medical journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Abstract Objective This study aimed to compare the in vitro effects of bone marrow mesenchymal stem cells (BM-MSCs) and adipose tissue (AT-MSCs) mitigating intrauterine growth retardation caused by oxidative stress. Materials Method 9.5-day-old embryos from Wistar albino pregnant rats were exposed hypoxia treated with BM-MSCs or AT-MSCs embryo culture. At 11.5 days, yolk sacs evaluated morphologically histologically assess developmental differences between groups. Results Hypoxia induced angiogenesis- neurogenesis-related anomalies. Stem cell treatments (H + BM-MSC, H AT-MSC) significantly improved embryonic development compared group ( p < 0.05 ). Although cell-treated lagged slightly behind controls under normoxia > ), both BM-MSC AT-MSC applications mitigated hypoxia-related defects. Notably, showed superior results closer normoxic control group. Conclusion demonstrated a more effective improvement sac hypoxic conditions. These findings suggest that therapy could offer promising approach treat angiogenetic neurogenetic disorders

Язык: Английский

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Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study

Ultrastructural Pathology, Год журнала: 2025, Номер unknown, С. 1 - 8

Опубликована: Апрель 17, 2025

The development of new strategies to raise the survival and viability transplanted mesenchymal stem cells (MSCs) is very important for therapeutic potential cells. natural flavonoid myricetin has anticancer, antioxidant, anti-inflammatory antiapoptotic effects. effects on human umbilical cord-derived MSCs (HUC-MSCs) induced oxidative stress with hydrogen peroxide (H2O2) were evaluated by transmission electron microscopy (TEM) immunocytochemistry (ICC) staining. Myricetin showed an increase in number live cells, a decrease caspase-3 tumor necrosis factor-α (TNF-α) ICC staining intensity, translocase mitochondrial inner membrane 17 (TIM17) degeneration cell ultrastructure TEM against damage HUC-MSCs. results suggest that prevents stress-induced apoptosis inflammation can be combined HUC-MSCs culture considered as supportive alternative treatment option.

Язык: Английский

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Modulation of Mesenchymal Stem Cells for Enhanced Therapeutic Utility in Ischemic Vascular Diseases

International Journal of Molecular Sciences, Год журнала: 2021, Номер 23(1), С. 249 - 249

Опубликована: Дек. 27, 2021

Mesenchymal stem cells are multipotent isolated from various tissue sources, including but not limited to bone marrow, adipose, umbilical cord, and Wharton Jelly. Although cell-mediated mechanisms have been reported, the therapeutic effect of MSCs is now recognized be primarily mediated via paracrine effects through secretion bioactive molecules, known as “secretome”. The regenerative benefit secretome has attributed trophic factors cytokines that play neuroprotective, anti-angiogenic/pro-angiogenic, anti-inflammatory, immune-modulatory roles. advancement autologous therapy can hindered when introduced back into a hostile/disease environment. Barriers include impaired endogenous function, post-transplantation cell viability, altered efficiency. secretome-based therapeutics gained popularity, many translational hurdles, heterogeneity MSCs, proliferation potential, complex nature secretome, impeded progress. This review will discuss experimental clinical impact restoring functional capabilities prior transplantation progress in therapies involving extracellular vesicles. Modulation utilization MSCs–secretome most likely serve an effective strategy for promoting their ultimate success modulators.

Язык: Английский

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Enhancement of de novo lipogenesis by the IDH1 and IDH2-dependent reverse TCA cycle maintains the growth and angiogenic capacity of bone marrow-derived endothelial progenitor cells under hypoxia

Free Radical Biology and Medicine, Год журнала: 2024, Номер 213, С. 327 - 342

Опубликована: Янв. 26, 2024

Язык: Английский

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Adipose-derived Mesenchymal Stem Cells are Ideal for the Cell-based Treatment of Refractory Wounds: Strong Potential for Angiogenesis

Stem Cell Reviews and Reports, Год журнала: 2023, Номер 20(1), С. 313 - 328

Опубликована: Окт. 24, 2023

Язык: Английский

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Extracellular vesicle: A magic lamp to treat skin aging, refractory wound, and pigmented dermatosis?

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Ноя. 7, 2022

Exposure of the skin to an external stimulus may lead a series irreversible dysfunctions, such as aging, refractory wounds, and pigmented dermatosis. Nowadays, many cutaneous treatments have failed strike balance between cosmetic needs medical recovery. Extracellular vesicles (EVs) are one most promising therapeutic tools. EVs cell-derived nanoparticles that can carry variety cargoes, nucleic acids, lipids, proteins. They also ability communicate with neighboring or distant cells. A growing body evidence suggests play significant role in repair. We summarize current findings EV therapy wound, dermatosis describe novel engineering strategies for optimizing function outcomes.

Язык: Английский

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The Therapeutic Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cell Conditioned Medium on Experimental Colitis Was Improved by the Serum From Colitis Rats

Frontiers in Bioengineering and Biotechnology, Год журнала: 2021, Номер 9

Опубликована: Сен. 17, 2021

Adipose derived mesenchymal stem cells (AD-MSCs) have shown therapeutic potential in treatments of inflammatory bowel disease (IBD). Due to the harsh host environment and poor survival cells, controversy concerning homing, proliferation differentiation MSCs lesion tissue still remains. It has been reported that conditioned media from could improve colitis, whereas efficiency be significantly elevated by stimulation pro-cytokines. In this study, we pre-treated adipose with serum colitis rats then activated (CM-AcMSC) were collected. To compare effects CM-MSC CM-AcMSC on IBD, constructed dextran sodium sulphate (DSS)-induced rat models. The was induced administrating 5% DSS drinking water for 10 days, symptoms recorded daily. colon histopathological changes observed different staining methods (H&E PAS). expression levels MUC2 tight junctions (TJs) determined RT-qPCR. cytokines analyzed ELISA western blot analysis. Our findings suggested more effective ameliorating clinical features histological damage scores. Treatment increased TJs suppressed production pro-inflammatory colonic tissues rats. inhibitory responses mediated NF-κB signaling pathway. These results pre-activation promote paracrine factors medium

Язык: Английский

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