Advances in experimental medicine and biology,
Год журнала:
2023,
Номер
unknown, С. 49 - 70
Опубликована: Янв. 1, 2023
Abstract
Transient,
tissue-specific,
embryonic
progenitors
are
important
cell
populations
in
vertebrate
development.
In
the
course
of
respiratory
system
development,
multipotent
mesenchymal
and
epithelial
drive
diversification
fates
that
results
to
plethora
types
compose
airways
alveolar
space
adult
lungs.
Use
mouse
genetic
models,
including
lineage
tracing
loss-of-function
studies,
has
elucidated
signaling
pathways
guide
proliferation
differentiation
lung
as
well
transcription
factors
underlie
progenitor
identity.
Furthermore,
pluripotent
stem
cell-derived
ex
vivo
expanded
offer
novel,
tractable,
high-fidelity
systems
allow
for
mechanistic
studies
fate
decisions
developmental
processes.
As
our
understanding
biology
deepens,
we
move
closer
goal
vitro
organogenesis
resulting
applications
medicine.
Frontiers in Veterinary Science,
Год журнала:
2023,
Номер
10
Опубликована: Янв. 17, 2023
Regenerative
medicine
has
opened
the
door
to
exploration
of
new
therapeutic
methods
for
treatment
various
diseases,
especially
those
associated
with
local
or
general
disregulation
immune
system.
In
pulmonary
strategies
have
emerged
that
are
aimed
at
restoring
functional
lung
tissue
rather
than
alleviating
symptoms.
These
focus
on
regeneration
using
stem
cells
and/or
their
derivatives
replacement
dysfunctional
biomedical
engineering.
Animal
health
can
directly
benefit
from
regenerative
therapy
and
also
serve
as
a
translational
experimental
model
human
disease.
Several
clinical
trials
been
conducted
evaluate
effects
cellular
inflammatory
disease
in
animals.
Data
reported
date
show
several
beneficial
ex
vivo
models;
however,
our
understanding
mechanisms
therapies
exert
diseased
tissues
remains
incomplete.
Heliyon,
Год журнала:
2023,
Номер
9(9), С. e19601 - e19601
Опубликована: Авг. 29, 2023
The
lack
of
physiologically
relevant
in
vitro
models
has
hampered
progress
understanding
human
lung
development
and
disease.
Here,
we
describe
a
protocol
which
induced
pluripotent
stem
cells
(hiPSCs)
undergo
stepwise
differentiation
into
definitive
endoderm
(>88%
population)
to
three-dimensional
(3D)
organoids
(LORGs),
contain
both
epithelial
mesenchymal
cellular
architecture
display
proximal
distal
airway
patterning.
These
LORGs
can
maintained
for
more
than
90
days
by
re-embedding
the
Matrigel.
We
show
utility
disease
modeling
drug
screening
infection
with
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
treatment
antiviral
drugs.
Advances in experimental medicine and biology,
Год журнала:
2023,
Номер
unknown, С. 49 - 70
Опубликована: Янв. 1, 2023
Abstract
Transient,
tissue-specific,
embryonic
progenitors
are
important
cell
populations
in
vertebrate
development.
In
the
course
of
respiratory
system
development,
multipotent
mesenchymal
and
epithelial
drive
diversification
fates
that
results
to
plethora
types
compose
airways
alveolar
space
adult
lungs.
Use
mouse
genetic
models,
including
lineage
tracing
loss-of-function
studies,
has
elucidated
signaling
pathways
guide
proliferation
differentiation
lung
as
well
transcription
factors
underlie
progenitor
identity.
Furthermore,
pluripotent
stem
cell-derived
ex
vivo
expanded
offer
novel,
tractable,
high-fidelity
systems
allow
for
mechanistic
studies
fate
decisions
developmental
processes.
As
our
understanding
biology
deepens,
we
move
closer
goal
vitro
organogenesis
resulting
applications
medicine.
