ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(49), С. 67424 - 67443
Опубликована: Ноя. 27, 2024
Pyroptosis
has
gained
attention
for
its
potential
to
reinvigorate
the
immune
system
within
tumor
microenvironment.
However,
current
approaches
employing
pyroptosis
inducers
suffer
from
limitations.
They
primarily
rely
on
single
agents,
lack
precise
targeting,
and
potentially
disrupt
intricate
bone
formation
microenvironment,
hindering
local
repair
of
tumor-induced
defects.
Therefore,
a
therapeutic
strategy
is
urgently
needed
that
can
effectively
trigger
while
simultaneously
promoting
regeneration.
This
research
introduces
an
all-in-one
construct
designed
address
these
It
combines
cell-camouflaged
shell
with
autosynergistic
reactive
oxygen
species
(ROS)
generating
polymer.
incorporates
hollow
core
manganese
dioxide
(HMnO2)
embedded
photosensitizer
IR780
disguised
by
cell
membrane
M1
macrophage.
The
macrophage
grants
stealth-like
properties,
enabling
it
accumulate
selectively
at
site.
Upon
laser
irradiation,
acts
as
exogenous
ROS
generation
converting
light
energy
into
heat.
Additionally,
structure
HMnO2
serves
efficient
carrier
IR780.
Furthermore,
Mn4+
ions
released
deplete
glutathione
(GSH)
tumor,
further
amplifying
production.
synergistic
cascade
ultimately
culminates
in
induction
through
caspase-3-mediated
cleavage
gasdermin
E
(GSDME)
upon
activation.
Meanwhile,
depletion
GSH
microenvironment
(TME)
leads
Mn2+
ions.
These
establish
supportive
milieu,
which
promotes
transformation
marrow
mesenchymal
stem
cells
(BMSCs)
mature
cells.
This,
turn,
defects
rat
femurs.
Our
findings
strongly
indicate
may
be
osteosarcoma
treatment,
presents
robust
versatile
approach
targeted
therapy
tissue
regeneration
this
patient
population.
Pharmacological Research,
Год журнала:
2024,
Номер
208, С. 107383 - 107383
Опубликована: Авг. 28, 2024
Mitochondria
exhibit
heterogeneous
shapes
and
networks
within
among
cell
types
tissues,
also
in
normal
or
osteoporotic
bone
tissues
with
complex
types.
This
dynamic
characteristic
is
determined
by
the
high
plasticity
provided
mitochondrial
dynamics
stemmed
from
responding
to
survival
functional
requirements
of
various
cells
a
specific
microenvironments.
In
contrast,
dysfunction,
induced
dysregulation
dynamics,
may
act
as
trigger
death
signals,
including
common
apoptosis
other
forms
programmed
(PCD).
These
PCD
processes
consisting
tightly
structured
cascade
gene
expression
events,
can
further
influence
remodeling
facilitating
cells.
Mitochondrial
therefore,
drive
stand
at
crossroads
life
integrating
external
signals
altering
metabolism,
shape,
signal-response
properties
mitochondria.
implies
that
targeting
displays
significant
potential
treatment
osteoporosis.
Considerable
effort
has
been
made
osteoporosis
emphasize
parallel
roles
mitochondria
regulating
energy
calcium
signal
transduction,
oxidative
stress,
inflammation,
death.
However,
emerging
field
dynamics-related
not
well
understood.
Herein,
bridge
gap,
we
outline
latest
knowledge
on
during
Journal of Cellular and Molecular Medicine,
Год журнала:
2024,
Номер
28(20)
Опубликована: Окт. 1, 2024
Abstract
Osteoporosis
(OP),
a
systemic
skeletal
disease,
is
characterized
by
low
bone
mass,
tissue
degradation
and
microarchitecture
disturbance.
Bazi
Bushen,
Chinese
patented
medicine,
has
been
demonstrated
to
be
effective
in
attenuating
OP,
but
the
pharmacological
mechanism
remains
predominantly
unclear.
In
this
study,
senescence‐accelerated
mouse
prone
6
(SAMP6)
model
was
used
explore
homeostasis
treated
intragastrically
for
9
weeks
with
Bushen.
vivo
experiments
showed
that
Bushen
treatment
not
only
upregulated
levels
of
mineral
density
content
also
increased
RUNX2
OSX.
Furthermore,
primary
culture
mesenchymal
stem
cells
(BMSCs)
SAMP6
mice
verify
effects
on
balance
differentiation
between
osteoblasts
adipocytes,
as
well
ROS
aging
levels.
Finally,
OP
investigated
through
network
pharmacology
experimental
verification,
we
found
could
significantly
orchestrate
attenuate
progression
stimulating
PI3K‐Akt
inhibiting
apoptosis.
summary,
our
work
sheds
light
first
evidence
attenuates
regulating
PI3K‐AKT
apoptosis
pathways
homeostasis.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(18), С. 13873 - 13873
Опубликована: Сен. 8, 2023
Chronic
apical
periodontitis
(CAP)
is
a
disease
with
characteristics
of
inflammation
and
bone
loss.
In
this
study,
our
objective
was
to
examine
the
function
small
extracellular
vesicles
(sEVs)
obtained
from
milk
in
encouraging
osteogenic
differentiation
inhibiting
by
miR-21
CAP.
The
expression
detected
using
qRT-PCR
human
CAP
samples.
impact
on
process
investigated
CCK-8,
qRT-PCR,
immunofluorescence
staining,
Western
blot
analysis.
evaluation
RAW
264.7
cell
polarization
assessment
inflammatory
factor
were
conducted
through
qRT-PCR.
influence
sEVs
MC3T3-E1
cells
examined,
particular
emphasis
involvement
miR-21.
samples,
decrease
observed.
MiR-21
increased
osteogenesis-related
genes
M2
while
decreasing
M1
cytokines.
Treatment
milk-derived
also
promoted
osteogenesis
inflammation.
Conversely,
addition
inhibitors
resulted
opposite
effects.
Our
results
indicated
that
derived
had
positive
effect
formation
activation
anti-inflammatory
(M2)
macrophages
simultaneously
reduced
regulating
Regenerative Therapy,
Год журнала:
2024,
Номер
26, С. 1168 - 1180
Опубликована: Июнь 1, 2024
Bone
marrow
mesenchymal
stem
cells
(BMSC)-derived
exosomes
(Exos)
are
important
in
promoting
bone
and
vascular
regeneration.
Antler
blood
(ALB)
is
a
valuable
traditional
Chinese
medicine
with
potent
regenerative
effects.
However,
there
still
lack
of
clarity
regarding
the
relationship
between
ALB
BMSC-Exos.
World Journal of Stem Cells,
Год журнала:
2024,
Номер
16(11), С. 906 - 925
Опубликована: Ноя. 25, 2024
Thin
endometrium
seriously
affects
endometrial
receptivity,
resulting
in
a
significant
reduction
embryo
implantation,
and
clinical
pregnancy
live
birth
rates,
there
is
no
gold
standard
for
treatment.
The
main
pathophysiological
characteristics
of
thin
are
increased
uterine
arterial
blood
flow
resistance,
angiodysplasia,
slow
growth
the
glandular
epithelium,
low
expression
vascular
endothelial
factor,
epithelial
cell
(EEC)
hypoxia
tissue
aplasia.
Human
umbilical
cord
mesenchymal
stem
cells
(HucMSCs)
promote
repair
regeneration
damaged
by
secreting
microRNA
(miRNA)-carrying
exosomes.
However,
initiation
mechanism
HucMSCs
to
has
not
yet
been
clarified.
To
determine
role
hypoxic-EEC-derived
exosomes
function
explore
potential
mechanism.
Exosomes
were
isolated
from
normal
EECs
(EEC-exs)
hypoxia-damaged
(EECD-exs),
before
characterization
using
Western
blotting,
nanoparticle-tracking
analysis,
transmission
electron
microscopy.
cocultured
with
EEC-exs
or
EECD-exs
differentially
expressed
miRNAs
determined
sequencing.
MiR-21-5p
miR-214-5p
inhibitors
miR-21-3p
mimics
transfected
into
treated
signal
transducer
activator
transcription
3
(STAT3)
STAT3
inhibitor.
HucMSC
migration
was
assessed
Transwell
wound
healing
assays.
Differentiation
detecting
markers
stromal
lineage
(Vimentin
CD13)
(CK19
CD9)
blotting
immunofluorescence.
binding
targets
validated
dual-luciferase
reporter
assay.
lowly
EECD-ex-pretreated
HucMSCs.
MiR-214-5p
miR-21-5p
facilitated
migratory
differentiative
potentials
targeted
protein
inhibitor
activated
STAT3,
respectively,
negatively
regulated
phospho-STAT3.
MiR-21-5p-
miR-214-5p-inhibitor-induced
promotive
effects
on
reversed
inhibition.
overexpression
repressed
differentiation,
while
activation
these
effects.
Low
miR-21-5p/miR-214-5p
promotes
differentiation
via
signaling.
Exosomal
miR-214-5p/miR-21-5p
may
as
valuable
endometrium.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(49), С. 67424 - 67443
Опубликована: Ноя. 27, 2024
Pyroptosis
has
gained
attention
for
its
potential
to
reinvigorate
the
immune
system
within
tumor
microenvironment.
However,
current
approaches
employing
pyroptosis
inducers
suffer
from
limitations.
They
primarily
rely
on
single
agents,
lack
precise
targeting,
and
potentially
disrupt
intricate
bone
formation
microenvironment,
hindering
local
repair
of
tumor-induced
defects.
Therefore,
a
therapeutic
strategy
is
urgently
needed
that
can
effectively
trigger
while
simultaneously
promoting
regeneration.
This
research
introduces
an
all-in-one
construct
designed
address
these
It
combines
cell-camouflaged
shell
with
autosynergistic
reactive
oxygen
species
(ROS)
generating
polymer.
incorporates
hollow
core
manganese
dioxide
(HMnO2)
embedded
photosensitizer
IR780
disguised
by
cell
membrane
M1
macrophage.
The
macrophage
grants
stealth-like
properties,
enabling
it
accumulate
selectively
at
site.
Upon
laser
irradiation,
acts
as
exogenous
ROS
generation
converting
light
energy
into
heat.
Additionally,
structure
HMnO2
serves
efficient
carrier
IR780.
Furthermore,
Mn4+
ions
released
deplete
glutathione
(GSH)
tumor,
further
amplifying
production.
synergistic
cascade
ultimately
culminates
in
induction
through
caspase-3-mediated
cleavage
gasdermin
E
(GSDME)
upon
activation.
Meanwhile,
depletion
GSH
microenvironment
(TME)
leads
Mn2+
ions.
These
establish
supportive
milieu,
which
promotes
transformation
marrow
mesenchymal
stem
cells
(BMSCs)
mature
cells.
This,
turn,
defects
rat
femurs.
Our
findings
strongly
indicate
may
be
osteosarcoma
treatment,
presents
robust
versatile
approach
targeted
therapy
tissue
regeneration
this
patient
population.
