Development of oxidative stress- and ferroptosis-related prognostic signature in gastric cancer and identification of CDH19 as a novel biomarker

Human Genomics, Год журнала: 2024, Номер 18(1)

Опубликована: Ноя. 5, 2024

Ferroptosis is a unique mode of cell death that iron-dependent and associated with oxidative stress lipid peroxidation. Oxidative ferroptosis are essential mechanisms leading to metabolic abnormalities in cells have been popular areas cancer research. Initially, 76 ferroptosis-related genes (OFRGs) were acquired by intersecting the gene sets from ferroptosis. Afterwards, optimal OFRGs screened using PPI networks, individuals separated into two OFRG subtypes (K = 2). Subsequently, we successfully constructed verified prognostic signature comprising SLC7A2, Cadherin 19 (CDH19), CCN1. To further uncover potential biomarkers gastric (GC), examined expression level CDH19, investigated effects knocking down CDH19 on biological behavior GC cells, explored whether involved processes. According findings, low-risk scoring group less infiltration immune suppressive fewer occurrences escape dysfunction, greater efficacy chemotherapy immunotherapy, better survival outcomes. The qRT-PCR assay indicated was significantly higher cells. Through experiments, demonstrated can affect transcription levels ACSL4 GPX4, increase intracellular iron ion concentration accumulation reactive oxygen species (ROS), inhibit proliferation migration We developed an OFRG-related predict prognosis treatment responsiveness identified as possible therapeutic target for GC.

Язык: Английский

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Construction of a novel mitochondrial oxidative stress-related genes prognostic system and molecular subtype characterization for breast cancer

Discover Oncology, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 8, 2024

Breast cancer (BRCA) is the most common malignant tumor among women, characterized by high incidence rates and mortality rates. Oxidative stress immunity, particularly in relation to mitochondria, have emerged as pivotal factors breast carcinogenesis. Nonetheless, limited research has explored specific contribution of mitochondrial oxidative prognosis BRCA.

Язык: Английский

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Multi-omics analysis deciphers intercellular communication regulating oxidative stress to promote oral squamous cell carcinoma progression

npj Precision Oncology, Год журнала: 2024, Номер 8(1)

Опубликована: Ноя. 21, 2024

Oral squamous cell carcinoma (OSCC) is a common malignant tumor in the head and neck, associated with high recurrence poor prognosis. We performed an integrated analysis of single-cell RNA spatial transcriptomic data from cancerous normal tissues to create comprehensive atlas epithelial cells cancer-associated fibroblasts (CAFs). Our findings show that AKR1C3+ cells, located at tumor's stromal front, exhibit significant copy number variation prognostic indicators, suggesting role invasion. also identified distinct group early-stage CAFs (named OSCC_Normal, characterized by ADH1B+, MFAP4+, PLA2G2A+) interact where OSCC_Normal may inhibit FOXO1 redox switch these via IGF1/IGF1R pathway, causing oxidative stress overload. Conversely, use ITGA6/ITGB4 receptor counteract effects promoting cancer This study unveils complex interactions within OSCC microenvironment.

Язык: Английский

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FAM3C Regulates Glioma Cell Proliferation, Invasion, Apoptosis, and Epithelial Mesenchymal Transition via the Notch Pathway

Cancer Medicine, Год журнала: 2024, Номер 13(23)

Опубликована: Дек. 1, 2024

ABSTRACT Background Previous studies have implicated the involvement of FAM3C in cancerous development and progression. Herein, we aimed to further investigate oncological mechanism FAM3C, specifically glioma. Methods We utilized open‐source bioinformatics tools platforms analyze transcriptional expression levels, prognosis, correlation with clinical variables gliomas, subsequently, hypothesize its potential molecular functions possibly associated signaling pathways. Following this, glioma tissues were obtained from resected specimens patients assess markers related epithelial–mesenchymal transition (EMT) Notch Furthermore, cell lines subjected treatments including siRNA knockdown, lentiviral overexpression, pathway blockade, enabling investigation vitro, particularly EMT pathways, as well effects on cancer proliferation, cycle progression, apoptosis, invasion, using assays such MMT proliferation assay, transwell migration, flow cytometry analysis. Finally, a mouse subcutaneous xenograft model was established explore integrative function growth vivo. Results The level correlated progression grade served prognostic indicator for poor patient outcomes. Subsequent experiments conducted lines, tumor tissues, models reinforced close association processes invasion. Additionally, it observed that is involved regulation pathway. Conclusions emerges candidate detection biomarker application. Its regulatory role modulation transition‐driven invasion migration via implies unveil novel therapeutic targets treatment.

Язык: Английский

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Intermittent Fasting (IF) Reduces Tumor Metastasis via Ras/MAPK, PI3K/AKT/mTOR, Wnt/<i>&#946;</i>-Catenin, and HGF/c-Met Pathways

Open Journal of Obstetrics and Gynecology, Год журнала: 2024, Номер 14(12), С. 1827 - 1840

Опубликована: Янв. 1, 2024

Язык: Английский

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