The Effect of Adipose-Derived Stem Cell (ADSC)-Exos on the Healing of Autologous Skin Grafts in Miniature Pigs

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 479 - 479

Опубликована: Янв. 8, 2025

The skin functions as the body's primary defense barrier; when compromised, it can lead to dehydration, infection, shock, or potentially life-threatening conditions. Miniature pigs exhibit characteristics and healing processes highly analogous humans. Mesenchymal stem cells contribute injury repair through a paracrine mechanism involving exosomes. This research examines whether adipose-derived MSC exosomes effectively enhance following autologous grafting in miniature pigs. It also compares roles distinctions of ADSCs ADSC-Exos inflammatory responses tissue regeneration. study found significantly reduced levels oxidative stress products pro-inflammatory factors, while antioxidant anti-inflammatory pro-regenerative factors were elevated, anti-regenerative factor decreased. Moreover, expression key markers-namely, PI3K, Akt, mTOR-in regeneration-associated signaling pathway increased. alterations these indicators indicate that regulate promote provides novel theoretical foundation for implementation acellular therapy clinical settings.

Язык: Английский

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Epidermal stem cell-derived extracellular vesicles induce fibroblasts mesenchymal-epidermal transition to alleviate hypertrophic scar via the miR-200s/ZEBs axis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Abstract Hypertrophic scar (HS) is a prevalent yet unresolved wound healing complication characterized by persistent hyperactive and proliferative fibroblasts, leading to excessive extracellular matrix (ECM) synthesis collagen contraction. Our previous studies have identified epidermal stem cells (ESCs) as critical for HS remodeling, with its vesicles (EVs) playing vital role. However, the specific mechanisms remain unclear. In this study, we first discovered that ESC-EVs could effectively induce mesenchymal-epidermal transition (MET) of fibroblasts (HSFs) inhibit their biological activity. Furthermore, next-generation sequencing multiplexed CRISPR/Cas9 system, elucidated therapeutic effect mediated miR-200 family (miR-200s) encapsulated in ESC-EVs, which targeted inhibited ZEB1 ZEB2 HSFs. This role mechanism been thoroughly validated both vitro cell experiments vivo rat tail (RHS) models. These findings not only shed light on previously unidentified HS, but also provide potential novel targets strategies precise treatment.

Язык: Английский

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Regenerative Medicine in Plastic Surgery: The Role of Stem Cells and Bioprinting

Regenesis repair rehabilitation., Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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