Roles of extracellular vesicles from different origins in metabolic-associated fatty liver disease: progress and perspectives

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 10, 2025

Metabolic-Associated Fatty Liver Disease (MAFLD) is the most common chronic liver disease worldwide, associated with systemic metabolic dysregulation. It can progress from simple hepatic steatosis (MAFL) to more severe conditions like Steatohepatitis (MASH), fibrosis, cirrhosis, and Hepatocellular Carcinoma (HCC). There a critical lack of reliable non-invasive diagnostic methods effective pharmaceutical treatments for MAFLD/MASH, emphasizing need further research. Extracellular vesicles (EVs) are nanoscale structures that play important roles in cell signaling by delivering bioactive molecules. However, there significant gap literature regarding EVs hosts, plants, microbiota MAFLD. This review explores potential various sources—host, microbiota—as biomarkers, therapeutic agents, drug carriers, treatment targets Firstly, host-derived extracellular MAFLD, focus on cell-type specific their components—proteins, miRNAs, lipids—for diagnosis monitoring were discussed. Moreover, it highlighted mesenchymal stem (MSC)-derived reducing lipid accumulation injury, immune cell-derived mitigating inflammation fibrosis. The also discussed use as carriers due ability deliver molecules impact mechanisms. Additionally, summarized research plant-derived EVs, which help reduce accumulation, inflammation, enhance gut barrier function Also, explored microbial-derived novel targets, particularly relation insulin resistance, dysfunction Overall, exploring diverse host, plant, sources this offers valuable insights into biomarkers strategies, could pave way options increasingly prevalent disease. Notably, challenges translating clinical practice thoroughly discussed, aiming provide possible directions strategies future

Язык: Английский

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Single-cell Raman spectroscopy as a novel platform for unveiling the heterogeneity of mesenchymal stem cells

Talanta, Год журнала: 2025, Номер 292, С. 127933 - 127933

Опубликована: Март 12, 2025

Язык: Английский

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Anti-Tumor Effects of Sheep Umbilical Cord Mesenchymal Stem Cells on Melanoma Cells

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 426 - 426

Опубликована: Янв. 6, 2025

Melanoma is among the most common malignancies and has recently exhibited increased resistance to treatments, resulting in a more aggressive disease course. Mesenchymal stem cells (MSCs) secrete cytokines both vivo vitro, which regulate tumor cell signaling pathways microenvironment, thereby influencing progression. This study investigates anti-melanogenesis effects of sheep umbilical cord mesenchymal (SUCMSCs) assess their potential application melanoma treatment. Our findings indicate that, SUCMSCs reduce melanin content tyrosinase activity, inhibit viability, proliferation, migration, invasion, promote apoptosis. Subsequent experiments confirmed that effectively suppress growth, histological analysis via HE staining revealed notable differences. Additionally, transcriptome sequencing indicated anti-tumor were primarily mediated through autophagy, apoptosis, TGF-β NF-κB pathways. The RT-qPCR validation results aligned with data. In summary, exert interaction multiple cytokines, demonstrating significant for

Язык: Английский

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Bone Marrow Mesenchymal Stem Cells Promote Repairing the Bruised Tissue via Regulating mRNA Expression of Molecular Biomarkers and the Apoptotic Rate

Cellular Physiology and Biochemistry, Год журнала: 2025, Номер 59(1), С. 1 - 20

Опубликована: Янв. 10, 2025

Bruise is the extravasation of blood that may be mild or severe. Bone marrow mesenchymal stem cells (BM-MSCs) are one most promising used in regenerative medicine for treating many disorders. We aimed to evaluate efficiency BM-MSCs cutaneous bruises. 78 male albino rats were equally divided into 3 groups, control group (G1), bruise wound (G2) and Bruised animals treated with (G3). The sequences color changes recorded. Animals sacrificed skin samples collected histopathological examination analyzing mRNA expression rate transforming growth factor- β (TGF-β), interleukin-6 (IL-6), tumor necrotic factor-α (TNF-α), Heat shock protein-90 α (HSP-90α), Metalloprotiens-9 (MMP-9), microRNA-21 (miR-21), which incorporated healing process apoptotic rat. Subcutaneous injection reduced intensity bruised skin, statistically significant upregulation TGF-β, TNF-α, HSP-90α, down-regulation MMP-9 miR-21 rate, reduction inflammatory cells. have a improvement bruises by regulating TGF-β- IL-6- TNF-α- HSP-90α- MMP-9- reducing cell infiltration.

Язык: Английский

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Clinical research progress of umbilical cord blood mesenchymal stem cells in Knee articular cartilage repair: A review

Medicine, Год журнала: 2025, Номер 104(6), С. e41402 - e41402

Опубликована: Фев. 7, 2025

Umbilical cord blood mesenchymal stem cells (UCB-MSCs) are a type of adult cell with multipotent differentiation potential and immunoregulatory functions, primarily found in neonatal blood. Due to their noninvasive collection method, abundance, ease preservation, UCB-MSCs represent promising biological material. This review examines the clinical research on knee articular cartilage repair, highlighting regenerative for treating joint defects. Our aim is provide insights into current applications propose directions future research, focusing optimizing use enhancing patient outcomes.

Язык: Английский

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Emerging Role of Mesenchymal Stromal Cell and Exosome Therapies in Treating Cognitive Impairment

Pharmaceutics, Год журнала: 2025, Номер 17(3), С. 284 - 284

Опубликована: Фев. 20, 2025

Cognitive aging, characterized by the gradual decline in cognitive functions such as memory, attention, and problem-solving, significantly impacts daily life. This is often accelerated neurodegenerative diseases, particularly Alzheimer’s Disease (AD) Parkinson’s (PD). AD marked accumulation of amyloid-beta plaques tau tangles, whereas PD involves degeneration dopaminergic neurons. Both conditions lead to severe impairment, greatly diminishing quality life for affected individuals. Recent advancements regenerative medicine have highlighted mesenchymal stromal cells (MSCs) their derived exosomes promising therapeutic options. MSCs possess regenerative, neuroprotective, immunomodulatory properties, which can promote neurogenesis, reduce inflammation, support neuronal health. Exosomes, nanosized vesicles from MSCs, provide an efficient means delivering bioactive molecules across blood–brain barrier, targeting underlying pathologies PD. While these therapies hold great promise, challenges variability MSC sources, optimal dosing, effective delivery methods need be addressed clinical application. The development robust protocols, along with rigorous trials, crucial validating safety efficacy exosome therapies. Future research should focus on overcoming barriers, optimizing treatment strategies, exploring integration lifestyle interventions. By addressing challenges, MSC- exosome-based could offer transformative solutions improving outcomes enhancing individuals aging diseases.

Язык: Английский

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Emerging role of mesenchymal cells in cardiac and cerebrovascular diseases: Physiology, pathology, and therapeutic implications

Vascular Pharmacology, Год журнала: 2025, Номер unknown, С. 107473 - 107473

Опубликована: Фев. 1, 2025

Язык: Английский

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Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMI

Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)

Опубликована: Март 5, 2025

Adipose-derived regenerative cells (ADRCs) also referred to as the stromal vascular fraction, provide an ample source of stem with widespread therapeutic use. Being heterogenous in nature, possibly affecting clinical outcome after cell treatment, ADRC- donor, -BMI, and -gender may have a large impact on ADRC composition quality but this remains largely unexplored. Herein, we comprehensive single-cell RNA sequencing mapping across two trial intervention studies found no gender- or BMI-related variations, except for minor female increase PI16/CD55-expressing cells. Indeed, heterogeneity was surprisingly minimal between donors. This provides important decision-making support adipose donor selection treatments, suggest that gender BMI should be regarded less influential.

Язык: Английский

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Pluripotent stem cell–derived mesenchymal stem cells for therapeutic applications, developmental study, and cancer research

Current Opinion in Genetics & Development, Год журнала: 2025, Номер 92, С. 102327 - 102327

Опубликована: Март 6, 2025

Язык: Английский

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Piezoelectric Biomaterials for Use in Bone Tissue Engineering—A Narrative Review

Journal of Biomedical Materials Research Part B Applied Biomaterials, Год журнала: 2025, Номер 113(4)

Опубликована: Март 17, 2025

ABSTRACT To examine natural bone's bioelectrical traits, notably its piezoelectricity, and to look into how these characteristics influence bone growth repair. In the context of exploring potential piezoelectric biomaterials, such as biopolymers bio‐ceramics, towards orthopedic regeneration applications, research seeks evaluate significance piezoelectricity‐driven osteogenesis. The paper reviews recent on electrical dielectric properties, surface polarization/electrical stimulation effects interacting with cell activity effectiveness biomaterials support tissues' regenerative process. study includes a number materials, collagen, polyvinylidene fluoride (PVDF) barium titanate. applications organic polymers, polymers are particularly highlighted. Piezoelectric being shown in studies enhance cellular metabolism vitro well promote tissues vivo, especially when paired electric field or interface polarization. bio‐ceramics like magnesium silicate titanate, collagen PVDF, have possibilities for applications. However, there several challenges regarding manufacturing specific compositions having desired properties. This review highlighted special emphasis bioceramics. Therefore, types materials huge because they can mimic properties allow better advances tissue engineering medicine. date, little is known about their mechanism action, modifications needed improve efficacy clinical uptake.

Язык: Английский

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