The Exploration of Disturbance of Capillary and Photoreceptor Communication Networks in Diabetic Retinopathy Through Single‐Cell RNA‐Seq

Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(5)

Опубликована: Март 1, 2025

This study investigates the differences in ligand-receptor interactions between communication network of vascular endothelial cells (ECs) and photoreceptor (PRCs)in diabetic retinopathy (DR) mechanism was verified by animal experiments. The GSE209872 data set, including retinal specimens from five Sprague-Dawley rats induced streptozotocin, obtained Gene Expression Omnibus. CM EC were extracted individually for reclustering, functional enrichment trajectory analyses. Cell analysis conducted to investigate altered signals significant interactions. Moreover, novel validated using immunofluorescence staining 2, 4 8 weeks DR model; treated with AAV-shANGPTL4, function detected Haematoxylin eosin (HE), TUNEL ELISA. expression Retina qPCR immunohistochemistry. Nine cell types determined DR. Cellular results revealed four signalling pathways, PTN, MK, ANGPTL CXCL, that significantly changed Furthermore, 3 pairs (Ptn-Ncl, Mkd-Ncl Angptl4-Sdc4) obviously upregulated ECs PRCs, which via model. After treatment thickness average density RGCs decreased (p < 0.05). showed knocking down ANGPTL4 reduced apoptosis 0.05), VEGF IGF-1 downregulated 0.01). ligand-receptors also Model PRCs demonstrate heterogeneities a improved as potential therapeutic target against

Язык: Английский

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Advances in the Study of the Role of Vascular Endothelial Cell Cadherin in Diabetic Retinopathy

Advances in Clinical Medicine, Год журнала: 2025, Номер 15(01), С. 13 - 19

Опубликована: Янв. 1, 2025

Язык: Английский

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Association of Albumin‐To‐Creatinine Ratio With Diabetic Retinopathy Among US Adults (NHANES 2009–2016)

Endocrinology Diabetes & Metabolism, Год журнала: 2025, Номер 8(1)

Опубликована: Янв. 1, 2025

This study investigates the relationship between albumin-to-creatinine ratio and diabetic retinopathy (DR) in US adults using NHANES data from 2009 to 2016. assesses predictive efficacy of urinary serum (UACR/SACR Ratio) against traditional biomarkers such as (SACR) (UACR) for evaluating DR risk. Additionally, explores potential these biomarkers, both individually combination with HbA1c, early detection risk stratification DR. cross-sectional analysed 2594 National Health Nutrition Examination Survey (NHANES 2009-2016). Multivariate logistic regression models, adjusted demographic (sex, age, race education) clinical factors (WBC, PLT, RDW, HBP CHD), examined associations Biomarkers were continuously quartiles assess dose-response relationships. Receiver operating characteristic (ROC) curve analysis evaluated accuracy individual combined models. Elevated SACR levels inversely related risk, while UACR showed a positive correlation. The UACR/SACR demonstrated superior capability compared alone. most accurate model SACR, UACR, HbA1c (AUC = 0.614), highlighting development complexity. Subgroup analyses revealed stronger participants aged < 60 years those hypertension (both p 0.05), more pronounced effects observed males Mexican Americans, lifestyle no significant modifying effect. Ratio emerged potentially predictor, particularly younger patients hypertension, suggesting its utility enhancing stratification. Comprehensive evaluation renal function glycaemic control considering age- comorbidity-specific patterns, could improve prediction management. Future longitudinal studies should validate findings, identified high-risk subgroups, investigate underlying mechanisms, advancing personalised management strategies.

Язык: Английский

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Ranibizumab with luseogliflozin in type 2 diabetes with diabetic macular oedema: A randomised clinical trial

Diabetes Obesity and Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Abstract Aims Anti‐vascular endothelial growth factor (VEGF) therapy is the standard treatment for diabetic macular oedema (DMO); however, unmet needs remain. This study aimed to assess effectiveness of sodium‐glucose cotransporter 2 inhibitors (SGLT2i) in treating DMO. Materials and Methods multicentre randomised open‐label trial included 60 patients with DMO who were eligible anti‐VEGF therapy. Patients receive luseogliflozin or glimepiride. Ranibizumab was administered initially target eye, additional doses per protocol. The number ranibizumab up week 48, re‐admission rates evaluated. Fellow eye injections also assessed. Results Sixty participants, mostly retinopathy half previously treated therapy, included. SGLT2i sulfonylurea (SU) groups achieved equivalent glycated haemoglobin, central retinal thickness (CRT), best‐corrected visual acuity improvements. Injection frequency similar between (SGLT2i vs. SU: 3.9 ± 0.7 4.7 times, p = 0.36). Re‐administration decreased significantly after fourth injection group ( 0.030, hazard ratio: 0.45, 95% confidence interval: 0.22–0.92). eyes showed significant CRT reduction fewer compared those SU (1.3 0.6 3.4 0.8, 0.016). Conclusions Although overall no difference, some responded favourably required injections. fellow suggests SGLT2i's efficacy early‐stage Trial Registration University Hospital Medical Information Network Clinical Registry (UMIN000033961); Japan Trials (jRCTs031180210).

Язык: Английский

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The Complex Role of Matrix Metalloproteinase-2 (MMP-2) in Health and Disease

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13691 - 13691

Опубликована: Дек. 21, 2024

Matrix metalloproteinase-2 (MMP-2), a zinc-dependent enzyme, plays critical role in the degradation and remodeling of extracellular matrix (ECM). As member gelatinase subgroup metalloproteinases, MMP-2 is involved variety physiological processes, including tissue repair, wound healing, angiogenesis, embryogenesis. It primarily responsible for type IV V collagen, fibronectin, laminin, elastin, which are essential components ECM. secreted as an inactive pro-enzyme (proMMP-2) activated through proteolytic cleavage, with its activity being precisely regulated by inhibitors metalloproteinases (TIMPs). Dysregulation has been linked to pathological conditions, cardiovascular diseases, diabetic complications, kidney cancer. In it contributes vascular remodeling, atherosclerosis, aneurysms, while fibrotic mediates excessive ECM leading scarring. diabetes, elevated exacerbates complications such nephropathy, retinopathy, disease. cancer, facilitates tumor invasion metastasis degrading promoting angiogenesis. Despite roles both targeting therapeutic purposes presents challenges due dual functions raising concerns about unplanned consequences impaired healing or damage. These underscore need future research focus on developing selective modulators that can balance their under specific disease environments. Clinical trials modulation highlight potential inhibitors, those MMP-2, reduce progression fibrosarcoma, breast, lung cancers. This paper reviews structure, function, regulation involvement pathogenesis, implications modulating activity.

Язык: Английский

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Identification and mechanistic analysis of neurovascular coupling related biomarkers for diabetic macular edema

Frontiers in Molecular Biosciences, Год журнала: 2024, Номер 11

Опубликована: Сен. 13, 2024

Diabetic macular edema (DME) is a major cause of vision loss in the sick with diabetic retinopathy. The occurrence DME closely related to breakdown neurovascular coupling; however, its underlying mechanism has not been fully elucidated. aim this study was investigate diagnostic biomarkers and potential molecular mechanisms associated coupling DME.

Язык: Английский

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