Harnessing hydrodynamics for high-yield production of extracellular vesicles from stem cells spheroids with specific cargo profiling
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 3, 2025
ABSTRACT
This
study
presents
a
novel
method
and
device
for
the
hydrodynamic
production
of
extracellular
vesicles
(EVs)
derived
from
biomimetic
multicellular
3D
spheroids,
enabling
high-throughput
particle
release
that
is
10
to
20
times
higher
than
in
non-stimulated
conditions.
The
facilitates
formation
spheroids
human
mesenchymal
stem
cells
(hMSCs),
offering
an
all-in-one
approach
both
spheroid
generation
EV
release.
Production
are
reduced
just
few
hours,
with
yield
further
increased
by
alternating
periods
high
flow
recovery
sequential
approach.
Using
this
system,
we
explored
impact
starvation
conditions
on
protein
cargo
EVs,
identifying
distinct
markers
through
proteomics.
Specifically,
stimulation
enriched
EVs
plasma
membrane-derived
mitochondrial
proteins,
revealing
divergent
biogenesis
pathways.
Importantly,
produced
exhibited
therapeutic
properties,
demonstrated
effects
wound
healing,
angiogenesis,
anti-inflammatory
responses,
some
showing
enhanced
efficacy
under
stimulation.
Язык: Английский
Akkermansia muciniphila extracellular vesicles: function and theranostic potential in disease
LabMed discovery.,
Год журнала:
2025,
Номер
unknown, С. 100060 - 100060
Опубликована: Март 1, 2025
Язык: Английский
Extracellular vesicles in cancer´s communication: messages we can read and how to answer
Molecular Cancer,
Год журнала:
2025,
Номер
24(1)
Опубликована: Март 19, 2025
Abstract
Extracellular
vesicles
(EVs)
are
emerging
as
critical
mediators
of
intercellular
communication
in
the
tumor
microenvironment
(TME),
profoundly
influencing
cancer
progression.
These
nano-sized
vesicles,
released
by
both
and
stromal
cells,
carry
a
diverse
cargo
proteins,
nucleic
acids,
lipids,
reflecting
dynamic
cellular
landscape
mediating
intricate
interactions
between
cells.
This
review
provides
comprehensive
overview
biogenesis,
composition,
functional
roles
EVs
cancer,
highlighting
their
significance
basic
research
clinical
applications.
We
discuss
how
cells
manipulate
EV
biogenesis
pathways
to
produce
enriched
with
pro-tumorigenic
molecules,
explore
specific
contributions
key
hallmarks
such
angiogenesis,
metastasis,
immune
evasion,
emphasizing
role
shaping
TME
driving
therapeutic
resistance.
Concurrently,
we
submit
recent
knowledge
on
can
serve
valuable
source
biomarkers
for
minimally
invasive
liquid
biopsies,
its
potential,
particularly
targeted
drug
delivery
vehicles
immunomodulatory
agents,
showcasing
promise
enhancing
efficacy
safety
treatments.
By
deciphering
messages
carried
EVs,
gain
deeper
understanding
biology
develop
more
effective
strategies
early
detection,
therapy,
immunotherapy,
paving
way
new
era
personalized
precise
medicine
potential
significantly
improve
patient
outcomes.
Язык: Английский
Extracellular Vesicles: Advanced Tools for Disease Diagnosis, Monitoring, and Therapies
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 189 - 189
Опубликована: Дек. 29, 2024
Extracellular
vesicles
(EVs)
are
a
heterogeneous
group
of
membrane-encapsulated
released
by
cells
into
the
extracellular
space.
They
play
crucial
role
in
intercellular
communication
transporting
bioactive
molecules
such
as
proteins,
lipids,
and
nucleic
acids.
EVs
can
be
detected
body
fluids,
including
blood
plasma,
urine,
saliva,
amniotic
fluid,
breast
milk,
pleural
ascites.
The
complexity
diversity
require
robust
standardized
approach.
By
adhering
to
protocols
guidelines,
researchers
ensure
consistency,
purity,
reproducibility
isolated
EVs,
facilitating
their
use
diagnostics,
therapies,
research.
Exosomes
microvesicles
represent
an
exciting
frontier
modern
medicine,
with
significant
potential
transform
diagnosis
treatment
various
diseases
important
personalized
medicine
precision
therapy.
primary
objective
this
review
is
provide
updated
analysis
significance
highlighting
mechanisms
action
exploring
applications
diseases.
Additionally,
addresses
existing
limitations
future
offering
practical
recommendations
resolve
current
challenges
enhance
viability
for
clinical
use.
This
comprehensive
approach
aims
bridge
gap
between
EV
research
its
application
healthcare.
Язык: Английский
Emerging Cancer Immunotherapies: Cutting-Edge Advances and Innovations in Development
Medical Sciences,
Год журнала:
2024,
Номер
12(3), С. 43 - 43
Опубликована: Авг. 28, 2024
The
rise
in
biological
therapies
has
revolutionized
oncology,
with
immunotherapy
leading
the
charge
through
breakthroughs
such
as
CAR-T
cell
therapy
for
melanoma
and
B-ALL.
Modified
bispecific
antibodies
cells
are
being
developed
to
enhance
their
effectiveness
further.
However,
currently
relies
on
a
costly
ex
vivo
manufacturing
process,
necessitating
alternative
strategies
overcome
this
bottleneck.
Targeted
viral
transduction
offers
promising
avenue
but
remains
under-optimized.
Additionally,
novel
approaches
emerging,
vaccine
boosting
of
strengthen
immune
response
against
tumors,
dendritic
cell-based
vaccines
under
investigation.
Beyond
cells,
mRNA
therapeutics
represent
another
avenue.
delivery
DNA/RNA
using
lipid
nanoparticles
(LNPs)
shows
potential,
LNPs
can
be
directed
T
cells.
Moreover,
CRISPR
editing
demonstrated
ability
precisely
edit
genome,
enhancing
effector
function
persistence
synthetic
Enveloped
vehicles
packaging
Cas9
modified
offer
virus-free
method
safe
effective
molecule
release.
While
platform
still
transduction,
from
healthy
donors
or
induced
pluripotent
stem
reduce
costs,
simplify
manufacturing,
expand
treatment
patients
low-quality
use
allogeneic
cancer
gained
attraction
its
potential
lower
costs
broaden
accessibility.
This
review
emphasizes
critical
improving
selectivity
efficacy
immunotherapies,
paving
way
more
targeted
successful
fight
cancer.
Язык: Английский
Exploring the potential of the convergence between extracellular vesicles and CAR technology as a novel immunotherapy approach
Journal of Extracellular Biology,
Год журнала:
2024,
Номер
3(9)
Опубликована: Сен. 1, 2024
Abstract
Cancer
therapy
is
a
dynamically
evolving
field,
witnessing
the
emergence
of
innovative
approaches
that
offer
promising
outlook
for
patients
grappling
with
persistent
disease.
Within
realm
therapeutic
exploration,
chimeric
antigen
receptor
(CAR)
T
cells
as
well
CAR
NK
cells,
have
surfaced
novel
approaches,
each
possessing
unique
attributes
and
transformative
potential.
Immune
engineered
to
express
CARs
recognizing
tumour‐specific
antigens,
shown
remarkable
promise
in
treating
terminal
cancers
by
combining
precision
antibody
specificity
potent
cytotoxic
function
cells.
However,
their
application
solid
tumours
still
its
nascent
stages,
presenting
major
challenges.
On
same
note,
distinct
immunotherapeutic
approach,
utilizing
on
providing
advantages
safety,
manufacturing
simplicity,
broader
scope
cancer
treatment.
Extracellular
vesicles
(EVs)
emerged
agents
due
ability
carry
crucial
biomarkers
biologically
active
molecules,
serving
vital
messengers
intercellular
communication
network.
In
context
cancer,
potential
EVs
lies
delivering
tumour‐suppressing
proteins,
nucleic
acid
components,
or
targeting
drugs
precision,
thereby
redefining
paradigm
medicine.
The
fusion
technology
capabilities
has
given
rise
new
frontier.
EVs,
leveraging
power
alleviate
challenges
associated
live‐cell
therapies.
are
suggested
reduce
side
effects
linked
cell
hold
revolutionize
penetrance
tumours.
act
carriers
pro‐apoptotic
molecules
RNA
enhancing
immune
responses
expanding
this
review
article,
we
navigate
dynamic
landscapes,
our
objective
being
evaluate
comparative
efficacy,
safety
profiles,
complexities,
clinical
applicability.
Язык: Английский
Biomimetic Nanovaccines Restore Immunosuppressive Tumor Antigen-Presenting Cells via the Saposin-Feeding Strategy
ACS Biomaterials Science & Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 19, 2024
Cancer
cell
membrane-derived
biomimetic
nanovaccines
have
shown
tremendous
potential
in
cancer
immunotherapy.
However,
their
efficacy
is
restricted
by
the
insufficient
cross-presentation
of
membrane-associated
antigens.
Saposins
(SAs),
which
are
vital
for
membrane
vesicle
disintegration
and
antigen
presentation,
severely
deficient
antigen-presenting
cells
(APCs)
within
tumors.
Herein,
we
propose
a
complementary
strategy
increasing
via
use
SAs.
Biomimetic
were
designed
using
shells
to
provide
comprehensive
array
tumor-associated
antigens
reactive
oxygen
species
(ROS)-responsive
nanoparticle
cores
that
allowed
codelivery
cytosine–guanine
dinucleotides
(CpGs)
The
ROS-responsive
highly
internalized
APCs,
enabled
release
CpGs
SAs
endo/lysosomes
APCs.
Furthermore,
increased
activation
immunosuppressive
APCs
enhanced
T-cell
priming
delivering
Consequently,
loaded
with
not
only
suppressed
tumor
growth
but
also
exhibited
excellent
therapeutic
effects
combination
immune
checkpoint
blockade
strategies.
Overall,
our
study
provides
insights
into
development
integrating
offers
promising
effective
Язык: Английский