New horizons for the role of RNA N6-methyladenosine modification in hepatocellular carcinoma

Acta Pharmacologica Sinica, Год журнала: 2024, Номер 45(6), С. 1130 - 1141

Опубликована: Янв. 9, 2024

Язык: Английский

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Transcriptome-wide study revealed m6A and miRNA regulation of embryonic breast muscle development in Wenchang chickens

Frontiers in Veterinary Science, Год журнала: 2022, Номер 9

Опубликована: Июль 26, 2022

N6-Methyladenosine (m6A) modification has been shown to play important role in skeletal muscle development. Wenchang chickens are commonly used as a high-quality animal model researching meat quality. However, there have no previous reports regarding the profile of m6A and its function embryonic breast development chickens. In this paper, we identified different developmental stages performed sequencing miRNA embryos. Embryo muscles were weighed stained with hematoxylin-eosin after hatching. We found that myofibers grew fast on 10th day hatching (E10) seldom proliferated beyond 19th (E19). A total 6,774 differentially methylated genes (DMGs) between E10 E19. For RNA-seq data, 5,586 expressed (DEGs). After overlapping DEGs DMGs, recorded 651 shared (DEMGs). Subsequently, miRNA-seq analysis obtained 495 miRNAs (DEMs). Then, overlapped DEMGs target DEMs 72 (called miRNA-m6A-genes study). GO KEGG results showed enriched many development-related pathways. Furthermore, chose WNT7B, key regulator development, perform IGV visualization levels WNT7B gene E19 significantly different. conclusion, miRNAs, conjunction modification, played The paper offer theoretical basis for study fat deposition

Язык: Английский

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Mechanism of N6-Methyladenosine Modification in the Pathogenesis of Depression

Molecular Neurobiology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Язык: Английский

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Chronic Dexamethasone Disturbs the Circadian Rhythm of Melatonin and Clock Genes in Goats

Animals, Год журнала: 2025, Номер 15(1), С. 115 - 115

Опубликована: Янв. 6, 2025

Dex is a drug commonly used as an immunosuppressive and anti-inflammatory agent in humans animals. GCs have profound impact on melatonin expression biological rhythm. However, the effect of chronic exposure to secretion clock gene ruminants still unclear. Ten goats were randomly divided into two groups: control group was injected with saline, Dex-treated intramuscularly daily for 21 d 0.2 mg/kg Dex. The rhythm plasma disturbed group, colon levels lower compared (p < 0.05). leads significant decrease Arylalkylamine N-acetyltransferase (AANAT), key synthase, pineal gland colon. Detecting intestinal leakage-related indices showed that diamine oxidase (DAO) lipopolysaccharide (LPS) content increased significantly We also detected genes associated rhythms plasma. In five tested circadian rhythms, but Clock, Cry1, Cry2, Per2 abolished or blunted by Protein CLOCK BMAL1 changed conclusion, above experimental results show disorder genes.

Язык: Английский

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Rethinking Poultry Welfare—Integrating Behavioral Science and Digital Innovations for Enhanced Animal Well-Being

Poultry, Год журнала: 2025, Номер 4(2), С. 20 - 20

Опубликована: Апрель 29, 2025

The relentless drive to meet global demand for poultry products has pushed rapid intensification in chicken farming, dramatically boosting efficiency and yield. Yet, these gains have exposed a host of complex welfare challenges that prompted scientific scrutiny ethical reflection. In this review, I critically evaluate recent innovations aimed at mitigating such concerns by drawing on advances behavioral science digital monitoring insights into biological adaptations. Specifically, focus four interconnected themes: First, spotlight the complexity avian sensory perception—encompassing vision, auditory capabilities, olfaction, tactile faculties—to underscore how lighting design, housing configurations, enrichment strategies can better align with birds’ unique worlds. Second, explore novel tools gauging emotional states cognition, ranging from cognitive bias tests developing protocols identifying pain or distress based facial cues. Third, examine transformative potential computer bioacoustics, sensor-based technologies continuous, automated tracking behavior physiological indicators commercial flocks. Fourth, assess data-driven management platforms, underpinned precision livestock deploy real-time optimize broad scale. Recognizing climate change evolving production environments intensify challenges, also investigate breeds resilient extreme conditions might open new avenues welfare-centered genetic approaches. While adoption cutting-edge techniques shown promise, significant hurdles persist regarding validation, standardization, acceptance. conclude truly sustainable progress hinges an interdisciplinary convergence ethology, neuroscience, engineering, data analytics, evolutionary biology—an integrative path not only refines assessment but reimagines ethically scientifically robust ways.

Язык: Английский

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Residue of Erycibe obtusifolia Benth as bedding materials improves welfare and microorganisms of chickens

British Poultry Science, Год журнала: 2025, Номер unknown, С. 1 - 11

Опубликована: Май 14, 2025

1. This study investigated the effect of Erycibe obtusifolia Benth (EOB) plant residue on welfare, stress-related gene expression as well microorganisms both gut and bedding materials chickens.2. In this study, a total 160, bearded chickens aged 80 d old were raised four types: EOB, rice husk (RH), EOB RH (EOB E) plus Enterococcus faecalis fermented (RHEOB). Twelve behaviours, foot pad dermatitis feather condition each group observed, caecal contents material collected for detection brain tissues related to fear, stress aggressive behaviour.3. Bedding had no (FPD), or abnormal behaviour. Sand bathing (p <0.05) affinity behaviour increased in birds with material. The expressions 5-HT, HTR1A CRH genes tissue affected by different types. mixture reduced relative abundance Brevibacterium, Streptococcus spp. probiotics Rombutz, Lactobacillus L. mucosus improved microflora contents.4. showed good application, mixed best effect.

Язык: Английский

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Constant light in early life induces fear-related behavior in chickens with suppressed melatonin secretion and disrupted hippocampal expression of clock- and BDNF-associated genes

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2022, Номер 13(1)

Опубликована: Июнь 21, 2022

Light management plays an important role in the growth and behavior of broiler chickens. Constant light early post hatch stage has been a common practice industry for improving performance, while whether how constant life affects chickens is rarely reported. In this study, newly hatched chicks were kept either (24 L:0 D, LL) or (12 L:12 LD) photoperiod 7 d then maintained 12 D thereafter until 21 days age. increased average daily feed intake but not body weight, which led to higher conversion ratio. Chickens LL group exhibited fear-related behaviors, was associated with corticosterone, lower melatonin 5-HT levels. Concurrently, exposure mRNA expression clock-related genes suppressed antioxidative hippocampus. Moreover, brain derived neurotrophic factor/extracellular signal-regulated kinase (BDNF/ERK) pathway hippocampus exposed first week hatching. These findings indicate that suppress secretion disrupts hippocampal involved circadian clock BDNF/ERK pathway, thereby contributing behaviors chicken.

Язык: Английский

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Hypothalamic expression analysis of m6A RNA methylation associated genes suggests a potential role of epitranscriptomics in sexual maturation of Atlantic salmon

Aquaculture, Год журнала: 2023, Номер 579, С. 740151 - 740151

Опубликована: Сен. 30, 2023

Язык: Английский

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Regulation and application of m6A modification in tumor immunity

Science China Life Sciences, Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

Язык: Английский

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Circadian Corticosterone Profile in Laying Hens (Gallus gallus domesticus)

Animals, Год журнала: 2024, Номер 14(6), С. 873 - 873

Опубликована: Март 12, 2024

Measurement of blood corticosterone concentrations has been established as an indicator for assessment acute distress. Therefore, knowledge on physiological fluctuations is required, but previous studies allow little conclusion daily in domestic chickens (Gallus gallus domesticus). To verify the presence a circadian rhythm socialized chickens, samples were taken at four-hour intervals from 12 laying hens kept groups four over three days, each. Prior to experiments, adapted repeated handling stress reduction. Corticosterone concentration was determined using radioimmunoassay. Blood sampling time and duration recorded, audio video recordings analyzed assess impact behavior concentrations. Despite individual fluctuations, most showed course with two peaks per day. Statistics revealed significant peak during day (between 12:00 p.m. 04:00 p.m.) tendency second night (12:00 a.m.). The not explained by daytime social needs be seen endophenotype. role nightly production investigated further studies. There might relation between reproduction since only hen showing eggs.

Язык: Английский

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