Most dominant roles of insect gut bacteria: digestion, detoxification, or essential nutrient provision?

Microbiome, Год журнала: 2020, Номер 8(1)

Опубликована: Март 16, 2020

The insect gut microbiota has been shown to contribute the host's digestion, detoxification, development, pathogen resistance, and physiology. However, there is poor information about ranking of these roles. Most results were obtained with cultivable bacteria, whereas bacterial physiology may be different between free-living midgut-colonizing bacteria. In this study, we provided both proteomic genomic evidence on roles bacteria by investigating anal droplets from a weevil, Cryptorhynchus lapathi.The lumen showed qualitatively quantitatively subsets communities. 16S rRNA sequencing that dominated Proteobacteria Bacteroidetes, are Proteobacteria. From droplets, enzymes involved in 31 basic belong 7 super identified Q-TOF MS. cooperation weevil its was determined reconstructing community pathway maps, which defined study. A score used rank data indicate most dominant role amino acid biosynthesis, followed protein energy metabolism, vitamin lipid plant secondary metabolite (PSM) degradation, carbohydrate while order PSM digestion. PCA form functional groups point view either or role, MFA variations among addition, data, analyzed HMFA method individual species, presented.The essential nutrient provisioning, digestion detoxification. plays pioneering diet mainly digests macromolecules into smaller molecules then digested

Язык: Английский

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Gut Microbiota Mediate Insecticide Resistance in the Diamondback Moth, Plutella xylostella (L.)

Frontiers in Microbiology, Год журнала: 2018, Номер 9

Опубликована: Янв. 22, 2018

The development of insecticide resistance in insect pests is a worldwide concern and elucidating the underlying mechanisms critical for effective crop protection. Recent studies have indicated potential links between gut microbiota these may apply to Diamondback moth, Plutella xylostella (L.), globally economically important pest cruciferous crops. We isolated Enterococcus sp. (Firmicutes), Enterobacter (Proteobacteria), Serratia (Proteobacteria) from guts P. analyzed effects on, resistance. enhanced widely used insecticide, chlorpyrifos, xylostella, while contrast, decreased all strains heat-killed bacteria had no effect. Importantly, direct degradation chlorpyrifos vitro was consistent among three bacteria. found that sp., vitamin C, acetylsalicylic acid similar on expression antimicrobial peptides. Expression cecropin down-regulated by two compounds, gloverin up-regulated. Bacteria were not associated with induced contrasting gene profiles compounds. Our confirmed play an role resistance, but main mechanism detoxification insecticides also suggest influence depend immune system. work advances understanding evolution this key highlights directions research into other species.

Язык: Английский

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Detoxifying symbiosis: microbe-mediated detoxification of phytotoxins and pesticides in insects

Natural Product Reports, Год журнала: 2018, Номер 35(5), С. 434 - 454

Опубликована: Янв. 1, 2018

Symbiotic microorganisms degrade natural and artificial toxic compounds, confer toxin resistance on insect hosts.

Язык: Английский

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A comprehensive review on the potential of microbial enzymes in multipollutant bioremediation: Mechanisms, challenges, and future prospects

Journal of Environmental Management, Год журнала: 2023, Номер 334, С. 117532 - 117532

Опубликована: Фев. 20, 2023

Язык: Английский

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Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

Frontiers in Physiology, Год журнала: 2023, Номер 13

Опубликована: Янв. 9, 2023

Threatening the global community is a wide variety of potential threats, most notably invasive pest species. Invasive species are non-native organisms that humans have either accidentally or intentionally spread to new regions. One effective and first lines control strategies for controlling pests application insecticides. These toxic chemicals employed get rid pests, but they pose great risks people, animals, plants. Pesticides heavily used in managing current era. Due overuse synthetic chemicals, numerous already developed resistance. The resistance development main reason failure manage Developing pesticide management techniques necessitates thorough understanding mechanisms through which insects acquire insecticide Insects use behavioral, biochemical, physiological, genetic, metabolic methods deal with can lead continuous overexpression detoxifying enzymes. An overabundance enzymes causes resistance, pesticides rendering them ineffective against pests. A key factor amplification certain enzymes, specifically esterases, Glutathione S-transferase, Cytochromes p450 monooxygenase, hydrolyses. Additionally, insect guts offer unique habitats microbial colonization, gut bacteria may serve their hosts useful services. Most importantly, detoxification insecticides leads development. complete knowledge could be very helpful coping challenges effectively developing Integrated Pest Management particularly at lowering risk chemical environmental contaminants resulting health issues, it also ways

Язык: Английский

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Resistance in the Genus Spodoptera: Key Insect Detoxification Genes

Insects, Год журнала: 2021, Номер 12(6), С. 544 - 544

Опубликована: Июнь 11, 2021

The genus Spodoptera (Lepidoptera: Noctuidae) includes species that are among the most important crop pests in world. These polyphagous able to feed on many plants, including corn, rice and cotton. In addition their ability adapt toxic compounds produced by they have developed resistance chemical insecticides used for control. One of main mechanisms insects become resistant involves detoxification enzymes. this review, we illustrate some examples role major families enzymes such as cytochromes P450, carboxyl/cholinesterases, glutathione S-transferases (GST) transporters ATP-binding cassette (ABC) insecticide resistance. We compare available data four species, exigua, S. frugiperda, littoralis litura. Molecular underlying involvement these genes will be described, duplication CYP9A cluster, over-expression GST epsilon or point mutations acetylcholinesterase ABCC2. This review is not intended exhaustive but highlight key roles certain genes.

Язык: Английский

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Functional and Structural Diversity of Insect Glutathione S-transferases in Xenobiotic Adaptation

International Journal of Biological Sciences, Год журнала: 2022, Номер 18(15), С. 5713 - 5723

Опубликована: Янв. 1, 2022

As a superfamily of multifunctional enzymes that is mainly associated with xenobiotic adaptation, glutathione S-transferases (GSTs) facilitate insects' survival under chemical stresses in their environment.GSTs confer adaptation through direct metabolism or sequestration xenobiotics, and/or indirectly by providing protection against oxidative stress induced exposure.In this article, comprehensive overview current understanding on the versatile functions insect GSTs detoxifying compounds presented.The diverse structures different classes GSTs, specifically spatial localization and composition amino acid residues constituted active sites are also summarized.Recent availability whole genome sequences numerous species, accompanied RNA interference, X-ray crystallography, enzyme kinetics site-directed mutagenesis techniques have significantly enhanced our functional structural diversity GSTs.

Язык: Английский

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Role of Insect Gut Microbiota in Pesticide Degradation: A Review

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Май 3, 2022

Insect pests cause significant agricultural and economic losses to crops worldwide due their destructive activities. Pesticides are designed be poisonous intentionally released into the environment combat menace caused by these noxious pests. To survive, insects can resist toxic substances introduced humans in form of pesticides. According recent findings, microbes that live insect as symbionts have recently been found protect hosts against toxins. Symbioses formed between various microbes, a defensive mechanism pathogens Insects’ guts provide unique conditions for microbial colonization, resident bacteria deliver numerous benefits hosts. Insects vary significantly reliance on gut basic functions. digestive tracts very different shape chemical properties, which big impact structure composition community. microbiota has contribute feeding, parasite pathogen protection, immune response modulation, pesticide breakdown. The current review will examine roles detoxification mechanisms behind development resistance better understand detoxifying agriculturally pest insects, we provided comprehensive information regarding role

Язык: Английский

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The gut symbiont Sphingomonas mediates imidacloprid resistance in the important agricultural insect pest Aphis gossypii Glover

BMC Biology, Год журнала: 2023, Номер 21(1)

Опубликована: Апрель 17, 2023

Abstract Background Neonicotinoid insecticides are applied worldwide for the control of agricultural insect pests. The evolution neonicotinoid resistance has led to failure pest in field. enhanced detoxifying enzyme activity and target mutations play important roles insects resistance. Emerging evidence indicates a central role gut symbiont pesticides. Existing reports suggest that symbiotic microorganisms could mediate pesticide by degrading pesticides Results 16S rDNA sequencing results showed richness diversity community between imidacloprid-resistant (IMI-R) imidacloprid-susceptible (IMI-S) strains cotton aphid Aphis gossypii no significant difference, while abundance Sphingomonas was significantly higher IMI-R strain. Antibiotic treatment deprived gut, followed an increase susceptibility imidacloprid IMI-S strain decreased as expected after supplementation with . In addition, nine field populations, which were all infected , increased different degrees antibiotics. Then, we demonstrated isolated from subsist only carbon source. metabolic efficiency reached 56% HPLC detection. This further proved A. hydroxylation nitroreduction. Conclusions Our findings detoxification properties, offer opportunity pests metabolize imidacloprid. These enriched our knowledge mechanisms insecticide provided new symbiont-based strategies insecticide-resistant high abundance.

Язык: Английский

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Detoxification of phoxim by a gut bacterium of Delia antiqua

The Science of The Total Environment, Год журнала: 2024, Номер 943, С. 173866 - 173866

Опубликована: Июнь 9, 2024

Язык: Английский

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