Accelerated spread of antibiotic resistance genes (ARGs) induced by non-antibiotic conditions: Roles and mechanisms

Water Research, Год журнала: 2022, Номер 224, С. 119060 - 119060

Опубликована: Сен. 6, 2022

Язык: Английский

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Wastewater surveillance of antibiotic-resistant bacterial pathogens: A systematic review

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Дек. 15, 2022

Infectious diseases caused by antibiotic-resistant bacterial (ARB) pathogens are a serious threat to human and animal health. The active surveillance of ARB using an integrated one-health approach can help reduce the emergence spread ARB, associated economic impact, guide antimicrobial stewardship programs. Wastewater (WWS) provides composite samples for total population, with easy access mixed community microbiome. This concept is emerging rapidly, but clinical utility, sensitivity, uniformity WWS remain poorly understood especially in relation evidence sewershed communities. Here, we systematically searched literature identify studies that have compared findings from antibiotic resistance genes (ARG) parallel, thereby evaluating how likely ARG relate cases Initially, 2,235 articles were obtained primary search keywords, 1,219 remained after de-duplication. Among these, 35 fulfilled criteria, additional 13 relevant included searching references literature. 48 papers, 34 used culture-based method, followed 11 metagenomics, three PCR-based methods. A 28 out conducted at single level, eight involved several countries, seven national or regional scales, five hospital levels. Our review revealed performance has been evaluated more frequently Escherichia coli, Enterococcus spp., other members family Enterobacteriaceae , not uniformly tested all pathogens. Many wastewater-based comparing evaluate public health risk ARB. Indeed, relating straightforward, as source wastewater cannot be only symptomatic individuals also asymptomatic carriers well sources. Further, varying fates each species within sewerage make aim connecting complicated. Therefore, future many AMR their one process simpler interpretation results easier.

Язык: Английский

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Detection and various environmental factors of antibiotic resistance gene horizontal transfer

Environmental Research, Год журнала: 2022, Номер 212, С. 113267 - 113267

Опубликована: Апрель 9, 2022

Язык: Английский

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Toward a Universal Unit for Quantification of Antibiotic Resistance Genes in Environmental Samples

Environmental Science & Technology, Год журнала: 2023, Номер 57(26), С. 9713 - 9721

Опубликована: Июнь 13, 2023

Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys human and animal under "One-Health" framework. However, there are substantial challenges comparing synthesizing results multiple studies that employ different test methods approaches bioinformatic analysis. In this article, we consider commonly used quantification units (ARG copy per cell, ARG genome, density, 16S rRNA gene, RPKM, coverage, PPM, etc.) for profiling ARGs suggest a universal unit cell) reporting such biological measurements samples improving comparability surveillance efforts.

Язык: Английский

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Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

Clinical Microbiology Reviews, Год журнала: 2023, Номер 37(1)

Опубликована: Дек. 14, 2023

SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.

Язык: Английский

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Environmental microbiome diversity and stability is a barrier to antimicrobial resistance gene accumulation

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Июнь 8, 2024

Abstract When antimicrobial resistant bacteria (ARB) and genes (ARGs) reach novel habitats, they can become part of the habitat’s microbiome in long term if are able to overcome biotic resilience towards immigration. This process should more difficult with increasing biodiversity, as exploitable niches a given habitat reduced for immigrants when diverse competitors present. Consequently, microbial diversity could provide natural barrier resistance by reducing persistence time immigrating ARB ARG. To test this hypothesis, pan-European sampling campaign was performed structured forest soil dynamic riverbed environments low anthropogenic impact. In soils, higher diversity, evenness richness were significantly negatively correlated relative abundance >85% ARGs. Furthermore, number detected ARGs per sample inversely diversity. However, no such effects present riverbeds. Hence, serve dissemination stationary, environments, where long-term, diversity-based against immigration evolve.

Язык: Английский

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Metagenome sequencing reveals shifts in phage-associated antibiotic resistance genes from influent to effluent in wastewater treatment plants

Water Research, Год журнала: 2024, Номер 253, С. 121289 - 121289

Опубликована: Фев. 8, 2024

Язык: Английский

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Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes

Environmental Science & Technology, Год журнала: 2022, Номер 56(21), С. 14982 - 14993

Опубликована: Июнь 27, 2022

Wastewater-based surveillance (WBS) for disease monitoring is highly promising but requires consistent methodologies that incorporate predetermined objectives, targets, and metrics. Herein, we describe a comprehensive metagenomics-based approach global of antibiotic resistance in sewage enables assessment 1) which genes (ARGs) are shared across regions/communities; 2) ARGs discriminatory; 3) factors associated with overall trends ARGs, such as concentrations. Across an internationally sourced transect samples collected using centralized, standardized protocol, ARG relative abundances (16S rRNA gene-normalized) were highest Hong Kong India lowest Sweden Switzerland, reflecting national policy, measured concentrations, metal genes. Asian versus European/US resistomes distinct, macrolide-lincosamide-streptogramin, phenicol, quinolone, tetracycline multidrug being discriminatory, respectively. Regional concentrations differed from expected public sales data. This could reflect unaccounted uses, captured only by the WBS approach. If properly benchmarked, might complement consumption statistics future. The defined herein demonstrates multisite comparability sensitivity to local/regional factors.

Язык: Английский

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The role and mechanism of quorum sensing on environmental antimicrobial resistance

Environmental Pollution, Год журнала: 2023, Номер 322, С. 121238 - 121238

Опубликована: Фев. 7, 2023

Язык: Английский

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Recommendations for the use of metagenomics for routine monitoring of antibiotic resistance in wastewater and impacted aquatic environments

Critical Reviews in Environmental Science and Technology, Год журнала: 2023, Номер 53(19), С. 1731 - 1756

Опубликована: Март 1, 2023

Shotgun metagenomic sequencing of the collective genomic information carried across microbial communities is emerging as a powerful approach for monitoring antibiotic resistance in environmental matrices. Metagenomics advantageous that known and putative genes (ARGs) (i.e., resistome) can be screened simultaneously without priori selection targets. Additionally, new ARGs are discovered catalogued, stored data reanalyzed to assess prevalence or pathogens. However, best practices generation processing needed support comparability space time. To reproducible downstream analysis, guidance first with respect sampling design, sample preservation storage, DNA extraction, library preparation, depth, experimental controls. Here we conducted systematic review current application metagenomics AR profiling wastewater, recycled water, surface water offer recommendations collection, production, analysis resulting data. Based on integrated findings reported 95 articles identified, field benchtop workflow discussed optimizing representativeness generated Through reanalysis 1474 publicly-available metagenomes, appropriate depths per environment uniform normalization strategies provided. Further, there opportunity harness quantitative capacity more overtly through inclusion The will amplify overall value within between study comparisons, now future.

Язык: Английский

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