Unveiling the Hidden Viromes Across the Animal Tree of Life: Insights from a Taxonomic Classification Pipeline Applied to Invertebrates of 31 Metazoan Phyla DOI Creative Commons
Pau Alfonso, Anamarija Butković, Rosa Fernández

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 6, 2023

Abstract Invertebrates constitute the majority of animal species on Earth, including most disease-causing agents or vectors, with more diverse viromes when compared to vertebrates. Recent advancements in high-throughput sequencing have significantly expanded our understanding invertebrate viruses, yet this knowledge remains biased toward a few well-studied lineages. In study, we analyze DNA and RNA for 31 phyla using 417 publicly available RNA-Seq datasets from environments marine-terrestrial marine-freshwater gradients. This study aims ( i ) estimate virome compositions at family level first time across Animal Tree Life, exploration several phyla, ii quantify diversity characterize structure invertebrate-virus interaction networks, iii investigate host phylum habitat influence differences. Results showed that set viral families eukaryotes, comprising Retroviridae , Flaviviridae giant were ubiquitous highly abundant. Nevertheless, some differences emerged between revealing instance less Ctenophora other phyla. Compositional analysis explained over five times variance composition than its habitat. Moreover, significant similarities observed phylogenetically related which could highlight co-evolution shaping viromes. Importance enhances global by characterizing previously unexamined lineages large number It showcases great within each investigates role communities. Furthermore, research identifies dominant virus invertebrates distinguishes analogous sets road towards deeper Life.

Язык: Английский

Multiple parallel origins of parasitic Marine Alveolates DOI Creative Commons
Corey C. Holt, Elisabeth Hehenberger, Denis V. Tikhonenkov

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Май 1, 2023

Abstract Microbial eukaryotes are important components of marine ecosystems, and the Marine Alveolates (MALVs) consistently one most abundant diverse groups in global environmental sequencing surveys. Relatives dinoflagellates, MALVs thought to be parasites animals other protists but absence data beyond ribosomal RNA gene sequences from all two species means much their biology evolution remain unknown. Here we show that evolved independently distinct, free-living ancestors parasitism twice, parallel, prior divergence core dinoflagellates. Phylogenomics shows subgroup (MALV II IV, or Syndiniales) related a novel lineage free-living, eukaryovorous predators, eleftherids, while I, now Ichthyodiniales) is Oxyrrhis marina . Reconstructing photosynthesis, plastids, early-diverging dinoflagellates number parallels with sister group, parasitic apicomplexans. In both groups, similar forms multiple times, photosynthesis was lost many plastid organelle infrequently, leaving no trace genome they ever existed.

Язык: Английский

Процитировано

0
Spatial compartmentalisation of bacteria in phoronid microbiomes DOI Creative Commons
Corey C. Holt,

Sahib Dhaliwal,

Ina Na

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Окт. 30, 2023

The phylum Phoronida comprises filter-feeding invertebrates that live in a protective tube sometimes reinforced with particulate material from the surrounding environments. Animals these characteristics make promising candidate hosts for symbiotic bacteria, given constant interactions various bacterial colonizers, yet phoronids are one of very few animal phyla no available microbiome data whatsoever. Here, by sequencing V4 region 16S rRNA gene, we compare microbiomes whole phoronids, including both and living tissues, those associated exclusively to isolated and/or naked inside. We also communities water. Phoronid specimens belonging same colony but collected month apart were significantly different, taxa previously reported association sediment found drive this difference. tubes similar composition animals. However, just over half bacteria specimens. In conclusion, harbour differ water, is not stable appears change relatively short time frame. Considering individual spatial/anatomical compartments, phoronid contributes most whole-animal microbiome.

Язык: Английский

Процитировано

0
Unveiling the Hidden Viromes Across the Animal Tree of Life: Insights from a Taxonomic Classification Pipeline Applied to Invertebrates of 31 Metazoan Phyla DOI Creative Commons
Pau Alfonso, Anamarija Butković, Rosa Fernández

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 6, 2023

Abstract Invertebrates constitute the majority of animal species on Earth, including most disease-causing agents or vectors, with more diverse viromes when compared to vertebrates. Recent advancements in high-throughput sequencing have significantly expanded our understanding invertebrate viruses, yet this knowledge remains biased toward a few well-studied lineages. In study, we analyze DNA and RNA for 31 phyla using 417 publicly available RNA-Seq datasets from environments marine-terrestrial marine-freshwater gradients. This study aims ( i ) estimate virome compositions at family level first time across Animal Tree Life, exploration several phyla, ii quantify diversity characterize structure invertebrate-virus interaction networks, iii investigate host phylum habitat influence differences. Results showed that set viral families eukaryotes, comprising Retroviridae , Flaviviridae giant were ubiquitous highly abundant. Nevertheless, some differences emerged between revealing instance less Ctenophora other phyla. Compositional analysis explained over five times variance composition than its habitat. Moreover, significant similarities observed phylogenetically related which could highlight co-evolution shaping viromes. Importance enhances global by characterizing previously unexamined lineages large number It showcases great within each investigates role communities. Furthermore, research identifies dominant virus invertebrates distinguishes analogous sets road towards deeper Life.

Язык: Английский

Процитировано

0