The Transformative Role of 3D Culture Models in Triple-Negative Breast Cancer Research

Cancers, Год журнала: 2024, Номер 16(10), С. 1859 - 1859

Опубликована: Май 13, 2024

Advancements in cell culturing techniques have allowed the development of three-dimensional (3D) culture models sourced directly from patients’ tissues and tumors, faithfully replicating native tissue environment. These provide a more clinically relevant platform for studying disease progression treatment responses compared to traditional two-dimensional (2D) models. Patient-derived organoids (PDOs) patient-derived xenograft (PDXOs) emerge as innovative 3D cancer capable accurately mimicking tumor’s unique features, enhancing our understanding tumor complexities, predicting clinical outcomes. Triple-negative breast (TNBC) poses significant challenges due its aggressive nature, propensity early metastasis, limited options. TNBC PDOs PDXOs significantly contributed comprehension TNBC, providing novel insights into underlying mechanism identifying potential therapeutic targets. This review explores transformative role various elucidating pathogenesis guiding strategies. It also provides an overview diverse models, derived lines highlighting their advantages challenges. Finally, it delves live-cell imaging techniques, endpoint assays, alternative media methodologies, such scaffold-free scaffold-based systems, essential advancing model research development.

Язык: Английский

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The mechanism underlying metastasis in triple-negative breast cancer: focusing on the interplay between ferroptosis, epithelial-mesenchymal transition, and non-coding RNAs

Frontiers in Pharmacology, Год журнала: 2025, Номер 15

Опубликована: Янв. 15, 2025

Triple-negative breast cancer (TNBC) is a type of with lack the expression estrogen receptor (ER), progesterone (PR), and human epidermal growth factor 2 (HER2). It most aggressive difficult to treat due its poor response treatments extremely invasive characteristics. The typical treatment for TNBC frequently results in relapse because particular choices. urgent focus on identifying workable effective target TNBC. Cancer metastasis significantly influenced by epithelial-mesenchymal transition (EMT). Ferroptosis an iron-dependent cell death form, changes key affect proliferation Several reports have established associations between EMT ferroptosis metastasis. Furthermore, non-coding RNA (ncRNA), which has been previously described, can also control Thus, this review, we summarize correlation pathways among ferroptosis, EMT, ncRNAs Also, aim find out novel strategy through ncRNA-ferroptosis-EMT axis.

Язык: Английский

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Unravelling key signaling pathways for the therapeutic targeting of non-small cell lung cancer

European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177494 - 177494

Опубликована: Март 1, 2025

Язык: Английский

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Glutathione Metabolism in Ferroptosis and Cancer Therapy

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217697 - 217697

Опубликована: Апрель 1, 2025

Язык: Английский

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RNF128 promotes gastric cancer progression by inhibiting autophagy-dependent ferroptosis through Beclin1 ubiquitination

Cell Death Discovery, Год журнала: 2025, Номер 11(1)

Опубликована: Апрель 19, 2025

Abstract As an important protein post-translational modification process, ubiquitination plays indispensable role in the regulation of gastric cancer (GC) occurrence and development. And recent studies have demonstrated that this is closely related to regulated cell death. This suggests our therapeutic approach inhibit malignant progression GC by regulating intracellular death mode through becomes possible. Although has been well described some tumorigenesis, its potential specific mechanisms are still unknown. In present study, we identified RNF128, E3 ubiquitin ligase with a RING structural domain, whose expression was significantly increased GC. In-depth showed knockdown RNF128 inhibited proliferation autophagic flux lipid peroxidation production, hypothesized autophagy-dependent ferroptosis might be main mediated RNF128. Mechanistically, directly binds ubiquitinates degradation Beclin1 PA domain inhibits Beclin1/solute transport family 7 member 11(SLC7A11)/glutathione peroxidase 4(GPX4) axis. Taken together, study reports for first time acts as tumor promoter GCs targeting Beclin1. These data provide new insights into activation expected strategy molecular therapy clinical patients.

Язык: Английский

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Overcoming Chemoresistance in Cancer: The Promise of Crizotinib

Cancers, Год журнала: 2024, Номер 16(13), С. 2479 - 2479

Опубликована: Июль 7, 2024

Chemoresistance is a major obstacle in cancer treatment, often leading to disease progression and poor outcomes. It arises through various mechanisms such as genetic mutations, drug efflux pumps, enhanced DNA repair, changes the tumor microenvironment. These processes allow cells survive despite chemotherapy, underscoring need for new strategies overcome resistance improve treatment efficacy. Crizotinib, first-generation multi-target kinase inhibitor, approved by FDA of ALK-positive or ROS1-positive non-small cell lung (NSCLC), refractory inflammatory (ALK)-positive myofibroblastic tumors (IMTs) relapsed/refractory anaplastic large lymphoma (ALCL). Crizotinib exists two enantiomeric forms: (R)-crizotinib its mirror image, (S)-crizotinib. assumed that R-isomer responsible carrying out reviewed here The S-isomer, on other hand, shows strong inhibition MTH1, an enzyme important repair mechanisms. Studies have shown crizotinib effective multi-kinase inhibitor targeting kinases c-Met, native/T315I Bcr/Abl, JAK2. Its mechanism action involves competitive ATP binding allosteric inhibition, particularly at Bcr/Abl. showed synergistic effects when combined with poly ADP ribose polymerase (PARP), especially ovarian harboring BRCA gene mutations. In addition, targets critical vulnerability many p53-mutated cancers. Unlike wild-type counterpart, p53 mutant promotes survival. can cause degradation mutant, sensitizing these DNA-damaging substances triggering apoptosis. Interestingly, reports demonstrated exhibits anti-bacterial activity, Gram-positive bacteria. Also, it active against drug-resistant strains. summary, exerts anti-tumor several mechanisms, including restoration sensitivity. potential combination therapies emphasized, cancers high prevalence triple-negative breast (TNBC) high-grade serous (HGSOC).

Язык: Английский

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Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells

Journal of Translational Medicine, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 29, 2024

Recycling of integrin via endosomal vesicles is critical for the migration cancer cells, which leads to metastasis pancreatic and devastating cancer-related death. So, new diagnostic therapeutic molecules target recycling need be developed.

Язык: Английский

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