Advancing Precision Medicine: The Role of Genetic Testing and Sequencing Technologies in Identifying Biological Markers for Rare Cancers DOI Creative Commons

Joviana Farhat,

Lara Alzyoud, Mohammad Alwahsh

и другие.

Cancer Medicine, Год журнала: 2025, Номер 14(8)

Опубликована: Апрель 1, 2025

ABSTRACT Background Genetic testing and sequencing technologies offer a comprehensive understanding of cancer genetics, providing rapid cost‐effective solutions. In particular, these advanced play an important role in assessing the complexities rare types affecting several systems including bone, endocrine, digestive, vascular, soft tissue. This review will explore how genetic have contributed to identification biomarkers across diagnostic, therapeutic, prognostic stages, thereby advancing PM. Methods A literature search was conducted PubMed (MEDLINE), EMBASE, Web Science using keywords related technologies, testing, cancer. There were no restrictions on language, methodology, age, or publication date. Both primary secondary research involving humans animals considered. Results practice, fluorescence situ hybridization, karyotype, microarrays other tests are mainly applied identify specific alterations mutations associated with progression. Sequencing such as next generation sequencing, polymerase chain reaction, whole genome exome enable analysis millions DNA fragments. These techniques assess structure, changes, gene expression profiles, epigenetic variations. Consequently, they help detect main intrinsic markers that crucial for personalizing diagnosis, treatment options, assessments, leading better patient prognosis. highlights why methods now considered tools research. However, still face multiple limitations, false positive results, limited precision, high costs. Conclusion significantly field by enabling key precision treatment, Despite existing their integration into clinical fields continues improve development personalized medicine strategies complex types.

Язык: Английский

CRISPR in clinical diagnostics: bridging the gap between research and practice DOI
Zainab Lafi,

Tha’er Ata,

S. Asha

и другие.

Bioanalysis, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Фев. 4, 2025

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has transformed molecular biology through its precise gene-editing capabilities. Beyond initial applications in genetic modification, emerged as a powerful tool diagnostics and biosensing. This review explores transition from genome editing to innovative detection methods, including nucleic acid identification, single nucleotide polymorphism (SNP) analysis, protein sensing. Advanced technologies such SHERLOCK DETECTR demonstrate CRISPR's potential for point-of-care diagnostics, enabling rapid highly sensitive detection. The integration of chemical modifications, CRISPR-Chip technology, enzymatic systems like Cas12a Cas13a enhances signal amplification efficiency. These advancements promise decentralized, real-time diagnostic solutions with significant implications global healthcare. Furthermore, the fusion artificial intelligence digital health platforms is paving way more accessible, cost-effective, scalable approaches, ultimately revolutionizing precision medicine.

Язык: Английский

Процитировано

1
Advancing Precision Medicine: The Role of Genetic Testing and Sequencing Technologies in Identifying Biological Markers for Rare Cancers DOI Creative Commons

Joviana Farhat,

Lara Alzyoud, Mohammad Alwahsh

и другие.

Cancer Medicine, Год журнала: 2025, Номер 14(8)

Опубликована: Апрель 1, 2025

ABSTRACT Background Genetic testing and sequencing technologies offer a comprehensive understanding of cancer genetics, providing rapid cost‐effective solutions. In particular, these advanced play an important role in assessing the complexities rare types affecting several systems including bone, endocrine, digestive, vascular, soft tissue. This review will explore how genetic have contributed to identification biomarkers across diagnostic, therapeutic, prognostic stages, thereby advancing PM. Methods A literature search was conducted PubMed (MEDLINE), EMBASE, Web Science using keywords related technologies, testing, cancer. There were no restrictions on language, methodology, age, or publication date. Both primary secondary research involving humans animals considered. Results practice, fluorescence situ hybridization, karyotype, microarrays other tests are mainly applied identify specific alterations mutations associated with progression. Sequencing such as next generation sequencing, polymerase chain reaction, whole genome exome enable analysis millions DNA fragments. These techniques assess structure, changes, gene expression profiles, epigenetic variations. Consequently, they help detect main intrinsic markers that crucial for personalizing diagnosis, treatment options, assessments, leading better patient prognosis. highlights why methods now considered tools research. However, still face multiple limitations, false positive results, limited precision, high costs. Conclusion significantly field by enabling key precision treatment, Despite existing their integration into clinical fields continues improve development personalized medicine strategies complex types.

Язык: Английский

Процитировано

0