Properties of Alzheimers disease brain-derived tau aggregates define tau processing by human astrocytes. DOI Creative Commons
Matthew J. Reid,

Melissa Salazar,

Claire Troakes

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 5, 2024

Summary The templated misfolding of tau proteins accounts for pathology spread in Alzheimer’s disease (AD). Post-translational modifications, including phosphorylation at specific residues, are closely linked with seeding ability and clinical progression. Increasing evidence supports a contributing role astrocytes spread. This study demonstrates that aggregates from postmortem AD brain internalized processed by human astrocytes. Differences the efficiency internalization, clearance and/or were noted, which may reflect molecular properties tau. Notably, we observed direct relationship between alterations handling astrocyte responses, evident transcriptomic data. Dysregulated genes include several previously identified as upregulated reactive brain, well being implicated pathological autophagy other pathways. provides new insights into complex interplay diversity responses AD.

Язык: Английский

Cellular and pathological functions of tau DOI
C Bravo, Sarah Naguib, Li Gan

и другие.

Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(11), С. 845 - 864

Опубликована: Июль 16, 2024

Язык: Английский

Процитировано

34

Inflammatory aspects of Alzheimer’s disease DOI
Pablo Botella Lucena, Michael T. Heneka

Acta Neuropathologica, Год журнала: 2024, Номер 148(1)

Опубликована: Авг. 28, 2024

Язык: Английский

Процитировано

23

Clathrin mediated endocytosis in Alzheimer’s disease: cell type specific involvement in amyloid beta pathology DOI Creative Commons

Sierra Jaye,

Ursula S Sandau,

Julie A. Saugstad

и другие.

Frontiers in Aging Neuroscience, Год журнала: 2024, Номер 16

Опубликована: Апрель 17, 2024

This review provides a comprehensive examination of the role clathrin-mediated endocytosis (CME) in Alzheimer's disease (AD) pathogenesis, emphasizing its impact across various cellular contexts beyond neuronal dysfunction. In neurons, dysregulated CME contributes to synaptic dysfunction, amyloid beta (Aβ) processing, and Tau pathology, highlighting involvement early AD pathogenesis. Furthermore, alterations extend non-neuronal cell types, including astrocytes microglia, which play crucial roles Aβ clearance neuroinflammation. Dysregulated these cells underscores broader implications pathophysiology. Despite significant progress, further research is needed elucidate precise mechanisms underlying dysregulation therapeutic implications. Overall, understanding complex interplay between diverse types holds promise for identifying novel targets interventions.

Язык: Английский

Процитировано

5

Extracellular PHF-tau modulates astrocyte mitochondrial dynamics and mediates neuronal connectivity DOI Creative Commons
Valentin Zufferey, Aatmika Barve,

Enea Parietti

и другие.

Translational Neurodegeneration, Год журнала: 2025, Номер 14(1)

Опубликована: Март 24, 2025

Abstract Background Tau is an intracellular protein that plays a crucial role in stabilizing microtubules. However, it can aggregate into various forms under pathological conditions and be secreted the brain parenchyma. While consequences of tau aggregation within neurons have been extensively studied, effects extracellular paired helical filaments (ePHF-tau) on astrocytes are still poorly understood. Methods This study examined effect human ePHF-tau (2N4R) primary cultures rat neuroglia, focusing changes neurites or synapses by microscopy analysis synaptosome mitochondria proteomic profiles after treatment. In addition, we monitored behavior separately over three days using high-speed imaging high-throughput acquisition analysis. Results was efficiently cleared two 3D neuron-astrocyte co-culture model. Treatment with led to rapid increase synaptic vesicle production active zones, suggesting potential excitotoxic response. Proteomic analyses synaptosomal mitochondrial fractions revealed distinct stress adaptations: exhibited elevated biogenesis turnover, whereas neuronal displayed only minor oxidative modifications. mixed culture model, overexpression 1N4R specifically triggered marked biogenesis, coinciding enhanced formation dendrites. Similarly, astrocyte-specific PGC1alpha produced comparable pattern production, indicating astrocytic adaptation may significantly influence function. Conclusions These findings suggest accumulation PHF-tau drives which regulation. astrocyte-mediated tauopathy highlights modulating dynamics response stress, opening avenues for therapeutic strategies aimed at mechanisms context neurodegenerative diseases.

Язык: Английский

Процитировано

0

Astrocyte–Neuron Interactions in Alzheimer’s Disease DOI
Clara Muñoz‐Castro, Alberto Serrano‐Pozo

Advances in neurobiology, Год журнала: 2024, Номер unknown, С. 345 - 382

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

3

Neuroinflammatory Biomarkers in Alzheimer’s Disease: From Pathophysiology to Clinical Implications DOI Open Access
Fausto Roveta, Lucrezia Bonino, Elisa Maria Piella

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 11941 - 11941

Опубликована: Ноя. 6, 2024

The identification of neuroinflammation as a critical factor in Alzheimer's disease (AD) has expanded the focus research beyond amyloid-β and tau pathology. neuroinflammatory fluid biomarkers GFAP, sTREM2, YKL-40 have gained attention for their potential early detection monitoring progression. Plasma GFAP demonstrated promise predicting conversion from mild cognitive impairment to AD dementia, while sTREM2 highlights microglial activation, although there are conflicting results regarding its dynamics pathogenesis. Advanced imaging techniques, such PET tracers targeting TSPO MAO-B, also been developed visualize glial activation vivo, offering spatial temporal insights into processes. However, clinical implementation these faces challenges due lack specificity, many them can be elevated other conditions. Therapeutic strategies emerging, with TREM2-targeting therapies antidiabetic drugs like GLP-1 receptor agonists showing modulating activity. Nevertheless, complexity neuroinflammation, which encompasses both protective harmful responses, necessitates further fully unravel role optimize therapeutic approaches AD.

Язык: Английский

Процитировано

3

Oligodendrocyte Dysfunction in Tauopathy: A Less Explored Area in Tau-Mediated Neurodegeneration DOI Creative Commons

Moumita Majumder,

Debashis Dutta

Cells, Год журнала: 2024, Номер 13(13), С. 1112 - 1112

Опубликована: Июнь 27, 2024

Aggregation of the microtubule-associated protein tau (MAPT) is hallmark pathology in a spectrum neurodegenerative disorders collectively called tauopathies. Physiologically, an inherent neuronal that plays important role assembly microtubules and axonal transport. However, disease-associated mutations this reduce its binding to microtubule components promote self-aggregation, leading formation tangles neurons. Tau also expressed oligodendrocytes, where it has significant developmental roles oligodendrocyte maturation myelin synthesis. Oligodendrocyte-specific pathology, form fibrils coiled coils, evident major tauopathies including progressive supranuclear palsy (PSP), corticobasal degeneration (CBD), Pick's disease (PiD). Multiple animal models tauopathy expressing mutant forms MAPT recapitulate oligodendroglial inclusions with potential cause degeneration/malfunction oligodendrocytes affecting sheath. Till now, mechanistic studies heavily concentrated on elucidating pathology. Therefore, more investigations are warranted comprehensively address tau-induced pathologies oligodendrocytes. The present review provides current knowledge available literature about intricate relations between health diseases.

Язык: Английский

Процитировано

2

Amyloid-β oligomers increase the binding and internalization of tau oligomers in human synapses DOI Creative Commons
Shrinath Kadamangudi, Michela Marcatti,

Wen-Ru Zhang

и другие.

Acta Neuropathologica, Год журнала: 2024, Номер 149(1)

Опубликована: Дек. 17, 2024

In Alzheimer's disease (AD), the propagation and spreading of CNS tau pathology closely correlates with cognitive decline, positioning as an attractive therapeutic target. Amyloid beta (Aβ) has been strongly implicated in driving spread, whereas primary tauopathies such age-related tauopathy (PART)—which lack Aβ pathology—exhibit limited spread minimal-to-no decline. Emerging evidence converges on a trans-synaptic mechanism facilitated by transfer misfolded aggregates (e.g. soluble oligomers). However, it is unclear whether oligomers modulate binding internalization human synapses. Our translationally focused paradigms utilize post-mortem brain specimens from Control, PART, AD patients. Synaptosomes isolated temporal cortex all three groups were incubated preformed recombinant tauO (rtauO), ± AβO (rAβO), oligomer binding/internalization was quantified via flow cytometry following proteinase K (PK) digestion surface-bound oligomers. TauO-synapse interactions visualized using EM immunogold. Brain-derived (BDTO) PART PBS-soluble hippocampal fractions co-immunoprecipitated analyzed mass spectrometry to compare synaptic interactomes secondary tauopathies, thereby inferring role Aβ. synaptosomes, enriched endogenous pathology, exhibited increased rtauO compared synaptosomes. This observation mirrored Control where rAβO significantly internalization. PK pre-treatment abolished this effect, implicating membrane proteins AβO-mediated While both BDTO broadly proteins, differential enrichment endocytic across pre- post-synaptic compartments, showed no significant enrichment. study demonstrates that enhance drive its through proteins. These findings offer novel mechanistic insights underlying pathological directly within synapses emphasize potential targeting Aβ-tau interactions.

Язык: Английский

Процитировано

1

Properties of Alzheimers disease brain-derived tau aggregates define tau processing by human astrocytes. DOI Creative Commons
Matthew J. Reid,

Melissa Salazar,

Claire Troakes

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 5, 2024

Summary The templated misfolding of tau proteins accounts for pathology spread in Alzheimer’s disease (AD). Post-translational modifications, including phosphorylation at specific residues, are closely linked with seeding ability and clinical progression. Increasing evidence supports a contributing role astrocytes spread. This study demonstrates that aggregates from postmortem AD brain internalized processed by human astrocytes. Differences the efficiency internalization, clearance and/or were noted, which may reflect molecular properties tau. Notably, we observed direct relationship between alterations handling astrocyte responses, evident transcriptomic data. Dysregulated genes include several previously identified as upregulated reactive brain, well being implicated pathological autophagy other pathways. provides new insights into complex interplay diversity responses AD.

Язык: Английский

Процитировано

0