Diversity of microbial communities in cigar filler leaves with different initial water contents analyzed based on high-throughput sequencing technology
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 7, 2025
To
study
the
composition
and
succession
of
bacterial
fungal
communities
during
fermentation
cigar
filler
leaves
with
varying
initial
water
contents,
high-throughput
sequencing
technology
was
used
to
sequence
16SrRNA
genes
ITS1
from
tobacco
leaf
samples.
This
followed
by
analyses
microbial
α-diversity,
community
structure,
function
prediction
based
on
data.
The
diversity
richness
decreased
over
time
under
different
content
conditions.
Among
18
samples,
predominant
phyla
identified
were
Proteobacteria,
Firmicutes,
Actinobacteria,
Ascomycota,
Basidiomycota,
leading
genera
being
Staphylococcus,
Sphingomonas,
Methylobacterium-Methylorubrum,
Pseudomonas,
Humicola.
Functional
predictions
for
bacteria
revealed
their
primary
involvement
in
carbohydrate,
lipid,
amino
acid
metabolism.
influenced
structure
relative
abundance
fermentation.
While
exhibited
a
similar
structural
composition,
there
notable
differences
abundance.
functional
results
PICRUSt
indicated
that
predicted
species
among
samples
minimal,
whereas
variations
more
pronounced
across
stages
contents.
Язык: Английский
Fast Pyrolysis of Cigarette and Cigar Leaves: Differential Analysis of Their Heavy Products by Ultrahigh‐Resolution Mass Spectrometry
Rapid Communications in Mass Spectrometry,
Год журнала:
2025,
Номер
39(10)
Опубликована: Фев. 18, 2025
ABSTRACT
Rationale
The
cigars
have
characteristic
flavors
in
smoke
when
compared
with
cigarettes,
and
from
various
origins
also
a
difference
taste.
However,
little
information
can
be
found
about
the
chemical
components
of
smokes
between
cigar
cigarette
as
well
cigars,
so
it
is
interesting
to
compare
their
pyrolysis
product
distribution.
Methods
leaves
were
pyrolyzed
microfluidized‐bed
reactor,
vapors
condensed
collected
using
cold
traps.
Mass
spectrometric
analysis
liquids
was
performed
utilizing
electrospray
ionization‐orbitrap
mass
spectrometry
both
positive
negative
ion
modes.
Results
spectra
condensable
products
three
tobacco
obtained
by
Orbitrap‐MS
ESI
DBE
values
(to
carbon
atom
number)
relative
distribution
nitrogen
or
oxygen
different
carefully
discussed.
Conclusion
main
relates
N
1
–N
2
class
compounds
high‐mass
range
highly
unsaturated
nitrogenated
compounds,
while
one
two
associated
C
x
H
y
O
z
5–9
30‐50
w
compounds.
Besides,
oxygenated
that
fall
into
H/C
>
O/C
<
ranges
are
for
Dominican
cigar.
Язык: Английский
Analysis of volatile compound metabolic profiles during the fermentation of filler tobacco leaves through integrated E-nose, GC–MS, GC-IMS, and sensory evaluation
Journal of Chromatography A,
Год журнала:
2024,
Номер
1737, С. 465472 - 465472
Опубликована: Окт. 30, 2024
Язык: Английский
Pyrolysis behavior of cigar wrapper, binder and filler
Journal of Thermal Analysis and Calorimetry,
Год журнала:
2024,
Номер
149(23), С. 14303 - 14312
Опубликована: Ноя. 11, 2024
Язык: Английский
Pyrolysis Behavior of Cigar Wrapper, Binder and Filler
Опубликована: Янв. 1, 2024
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DOI
Язык: Английский
The impact of gradient variable temperature fermentation on the quality of cigar tobacco leaves
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 13, 2024
Introduction
In
order
to
enhance
the
quality
of
cigar
tobacco
leaves
(CTLs),
a
gradient
variable
temperature
fermentation
approach
was
employed.
Methods
The
demonstrated
gradual
increase
from
low
(35
±
2°C)
moderate
(45
2°C),
and
then
high
(55
2°C).
Each
underwent
10-day
process,
resulting
in
total
duration
30
days.
Changes
sensory
evaluation,
chemical
composition,
bacterial
absolute
quantitative
structure
function
were
examined
throughout
process
fermentation.
Results
Compared
constant
fermentation,
improved
CTLs,
reduced
sugar
cembrane
degradation
products,
increased
amino
acid
contents.
It
resulted
significant
changes
quantity
but
had
no
effects
on
richness
diversity
communities.
results
correlation
analyses
showed
that
with
which
effected
by
predominant
microbes.
three-phase
model
characterize
alterations
CTLs.
Phase
I
(35°C)
microbial
stage,
during
there
decrease
both
number
microorganisms.
dominant
genera
shifted
Acinetobacter
Staphylococcus
Corynebacterium
,
reducing
sugar,
polyphenol
compound
score.
II
(45°C)
marked
an
enhancement
evaluation.
A
17
chemicals
significantly
decreased
six
increased,
decline
populations
persisted.
enhanced
relative
abundances
four
microecological
hubs,
namely
Oceanobacillus
Bacillus
potential
produce
protease
lipase
production
peptides,
acids,
organic
acid,
catabolizing
sugars
compounds,
through
carbohydrate
metabolism
metabolism,
III
(55°C)
indicated
mature
stage
highest
score
Eight
compositions
plamochromic
pigments
compounds
exhibited
decreases,
while
contents
increased.
Conclusion
positive
influence
CTL
providing
optimal
for
growth,
generation
quality-related
compositions.
Язык: Английский
Decoding the temperature and humidity-driven succession of microbial co-occurrence network and its impact on filler tobacco characteristics during agricultural fermentation
Industrial Crops and Products,
Год журнала:
2024,
Номер
224, С. 120401 - 120401
Опубликована: Дек. 27, 2024
Язык: Английский