Identification and experimental validation of diagnostic and prognostic genes CX3CR1, PID1 and PTGDS in sepsis and ARDS using bulk and single-cell transcriptomic analysis and machine learning DOI Creative Commons
Jie Jiang, Yan Chen, Yue Su

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Дек. 23, 2024

Background Sepsis is an uncontrolled reaction to infection that causes severe organ dysfunction and a primary cause of ARDS. Patients suffering both sepsis ARDS have poor prognosis high mortality. However, the mechanisms behind their simultaneous occurrence are unclear. Methods We acquired datasets from GEO Arrayexpress databases screened hub genes by WGCNA machine learning algorithm. For diagnosis prognosis, ROC curve survival analysis were used. performed GO, KEGG, GSEA, immune cell infiltration, drug prediction, molecular docking, transcription factor constructed PPI ceRNA networks explore these common Single-cell data compared profiles gene localization. Finally, RT-qPCR H&E staining confirmed reliability using PBMCs samples mouse models. Results identified 242 differentially expressed in showed turquoise module GSE95233 strongly linked while black GSE10474 associated with Using three methods (LASSO, random forest Boruta), we key CX3CR1, PID1 PTGDS. Models built them AUC values evaluations validated external datasets, accurately predicting further explored immunological landscape infiltration single-cell analysis. Then, ceRNA, predicted drugs docking analyzed. Ultimately, demonstrated differently human Conclusion This study signatures (CX3CR1, PTGDS) ARDS, patient samples. research may be valuable for identifying shared biological potential treatment targets diseases.

Язык: Английский

Impact of hyper- and hypothermia on cellular and whole-body physiology DOI Creative Commons
Toshiaki Iba, Yutaka Kondo, Cheryl L. Maier

и другие.

Journal of Intensive Care, Год журнала: 2025, Номер 13(1)

Опубликована: Янв. 13, 2025

The incidence of heat-related illnesses and heatstroke continues to rise amidst global warming. Hyperthermia triggers inflammation, coagulation, progressive multiorgan dysfunction, and, at levels above 40 °C, can even lead cell death. Blood cells, particularly granulocytes platelets, are highly sensitive heat, which promotes proinflammatory procoagulant changes. Key factors in pathophysiology involve mitochondrial thermal damage excessive oxidative stress, drive apoptosis necrosis. While the kinetics cellular from heat have been extensively studied, mechanisms driving heat-induced organ death not yet fully understood. Converse hyperthermia, hypothermia is generally protective, as seen therapeutic hypothermia. However, accidental presents another environmental threat due arrhythmias, cardiac arrest, coagulopathy. From a physiology perspective, supports homeostasis enhances preservation, aiding whole-body recovery following resuscitation. This review summarizes recent findings on temperature-related preservation suggests future research directions for understanding tempo-physiologic axis.

Язык: Английский

Процитировано

0
Sepsis-induced cardiac dysfunction: mitochondria and energy metabolism DOI Creative Commons

Xueting Yu,

Jie Gao, Chunxiang Zhang

и другие.

Intensive Care Medicine Experimental, Год журнала: 2025, Номер 13(1)

Опубликована: Фев. 18, 2025

Abstract Sepsis is a life-threatening multi-organ dysfunction syndrome caused by dysregulated host response to infection, posing significant global healthcare challenge. Sepsis-induced myocardial (SIMD) common complication of sepsis, significantly increasing mortality due its high energy demands and low compensatory reserves. The substantial mitochondrial damage rather than cell apoptosis in SIMD suggests disrupted cardiac metabolism as crucial pathophysiological mechanism. Therefore, we systematically reviewed the mechanisms underlying SIMD, including alterations substrates, excitation–contraction coupling processes, dysfunction, autophagy biogenesis, summarizing potential therapeutic targets within them.

Язык: Английский

Процитировано

0
Liver injury in sepsis: manifestations, mechanisms and emerging therapeutic strategies DOI Creative Commons

Xinqi Xu,

Tingyu Yang, Jie An

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 28, 2025

Sepsis is defined as a condition related to infection that manifests with multiorgan dysfunction, representing life-threatening state. Consequently, severe complications frequently occur, liver injury being one of the most prevalent serious sepsis. Liver dysfunction during sepsis serves an independent predictor mortality. This review provides comprehensive overview current research on sepsis-induced (SILI), encompassing clinical manifestations, diagnostic criteria, pathogenesis and therapeutic strategies associated this condition. SILI may manifest hypoxic hepatitis due ischemia shock, cholestasis resulting from abnormal bile metabolism, or duct sclerosis. The pathophysiology involves intricate interactions among inflammatory response, oxidative stress, cell death. All these factors complicate treatment represent potential targets for intervention. Furthermore, addresses limitations inherent in conventional therapies currently employed managing emphasizes novel targeted aimed at addressing fundamental mechanisms underlying

Язык: Английский

Процитировано

0
Apoptosis-Mechanisms, Regulation in Pathology, and Therapeutic Potential DOI

Irshad Ahmad Bhat,

Aalim Maqsood Bhat, Sheikh Tasduq Abdullah

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Апрель 2, 2025

Apoptosis, a highly regulated form of programmed cell death (PCD), is essential for development, tissue homeostasis, and the immune response. This self-destructive process characterized by distinct cellular changes, including membrane blebbing, chromatin condensation, DNA fragmentation, formation apoptotic bodies. Apoptosis can be triggered two primary signaling pathways: intrinsic (mitochondrial) pathway, responding to internal stress, extrinsic (death receptor) initiated external signals. Both pathways ultimately activate caspases, proteolytic enzymes that dismantle in an orderly manner, preventing inflammation. The pathway Bcl-2 protein family, balancing pro-apoptotic anti-apoptotic signals maintain integrity, while mitochondrial disruptions lead release cytochrome c activation downstream machinery. Dysregulation apoptosis linked various diseases, cancer, where defective processes allow uncontrolled proliferation, neurodegenerative disorders, excessive leads loss. review offers in-depth understanding apoptosis, its regulatory mechanisms offer crucial insights therapeutic approaches targeting diseases abnormal survival or death.

Язык: Английский

Процитировано

0
Identification and experimental validation of diagnostic and prognostic genes CX3CR1, PID1 and PTGDS in sepsis and ARDS using bulk and single-cell transcriptomic analysis and machine learning DOI Creative Commons
Jie Jiang, Yan Chen, Yue Su

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Дек. 23, 2024

Background Sepsis is an uncontrolled reaction to infection that causes severe organ dysfunction and a primary cause of ARDS. Patients suffering both sepsis ARDS have poor prognosis high mortality. However, the mechanisms behind their simultaneous occurrence are unclear. Methods We acquired datasets from GEO Arrayexpress databases screened hub genes by WGCNA machine learning algorithm. For diagnosis prognosis, ROC curve survival analysis were used. performed GO, KEGG, GSEA, immune cell infiltration, drug prediction, molecular docking, transcription factor constructed PPI ceRNA networks explore these common Single-cell data compared profiles gene localization. Finally, RT-qPCR H&E staining confirmed reliability using PBMCs samples mouse models. Results identified 242 differentially expressed in showed turquoise module GSE95233 strongly linked while black GSE10474 associated with Using three methods (LASSO, random forest Boruta), we key CX3CR1, PID1 PTGDS. Models built them AUC values evaluations validated external datasets, accurately predicting further explored immunological landscape infiltration single-cell analysis. Then, ceRNA, predicted drugs docking analyzed. Ultimately, demonstrated differently human Conclusion This study signatures (CX3CR1, PTGDS) ARDS, patient samples. research may be valuable for identifying shared biological potential treatment targets diseases.

Язык: Английский

Процитировано

0