Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 14, 2024

Background Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing barcodes (i.e., metabarcoding) enables simultaneous detection from multiple environmental samples. Short-read metabarcoding, using for example Illumina platforms, provides high depths but results in many unidentified taxa. Long-read may improve species genus assignments encompasses lower depth, limiting diversity coverage. In this study, we assessed seeds eleven angiosperm gymnosperm tree traditional culturing, short-read Oxford Nanopore Technologies long-read metabarcoding. We focused on seed-borne as understanding their potential impacts seedlings crucial securing plant health. compared 1) the number identity fungal genera between metabarcoding approaches culturing 2) alpha- beta-diversity methods, considering different hosts lifestyles.Results both short- datasets, similar numbers reads operational taxonomic units were assigned to comparable species. About one-third identified pathogens, followed by saprotrophs endophytes. overall revealed fewer genera, most datasets stemmed cultured per-sample than distinct communities. Host-dependent patterns observed across all with angiosperms harboring more taxa gymnosperms, community structuring host groups species, although differences stronger datasets.Conclusions captured host-dependent despite composition fungi. might be optimal biodiversity studies due higher resultant breadth diversity. As error rates decrease, reference databases expand, throughput improves, become a strong candidate future diagnostic Traditional captures remains valuable obtaining isolates further research.

Язык: Английский