Evaluation the role of Luteibacter pinisoli DP2-30 in mitigating pine wilt disease caused by Bursaphelenchus xylophilus through modulation of host microbiome

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 5, 2025

Pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus, poses a significant threat to forests worldwide. This study aimed isolate bacterial strains from rhizosphere of healthy Pinus massoniana and elucidate their biocontrol potential in mitigating PWD through direct nematicidal activity manipulation host microbiome. We successfully isolated rhizobacterium strain DP2-30 plants, which was identified as Luteibacter pinisoli on basis morphological, biochemical, molecular analyses. The fermentation filtrates displayed >95% (corrected mortality rate) PWN after 48 hours treatment. broth significantly inhibited egg hatching 49.38% 43.05%, respectively. Additionally, root drenching reduced severity seedlings (2 years old), with control effect 62.50%. Microbiome analyses revealed variations diversity, structure, relative abundance fungal communities plants combined treated (T2), solely (T1), (treated water). Bacterial phyla, Proteobacteria, Actinobacteriota, Chloroflexi, Acidobacteriota, Armatimonadota phyla Ascomycota, Basidiomycota Mortierellomycota were dominant all stem samples. application L. increased family Rhodanobacteraceae roots stems seedlings. intra-kingdom co-occurrence network analysis complexity networks but suggesting enhanced functional redundancy ecosystem stability. Overall, this highlights an effective agent against directly killing manipulating microbiota.

Язык: Английский

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Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Сен. 6, 2022

Plant health is of utmost importance for optimal agricultural production and sustainability. Unfortunately, biotic abiotic factors put a major constraint on crop safety productivity. diseases caused by oomycetes inflict serious damage to various crops. Moreover, the injudicious use chemical pesticides poses threats related pesticide resistance development in pathogens environmental pollution. Biocontrol offers an effective solution disease control; however, research biocontrol oomycete-related scarce. Thus, this study undertakes screening resources management plant diseases. In regard, 86 isolates Trichoderma spp. were assessed against Phytophthora nicotianae, P. capsici, Pythium vexans, ultimum, dissotocum through dual culture assay. Furthermore, antagonistic effect selected was studied tobacco black shank damping-off cucumber seedlings greenhouse. The relative control three strains AR-4, Tv-1, ST4-1 more than 60%, which not significantly different from 6.88 gl-1 fluopicolide-propamocarb. Whereas, AR-4 80.33% 82.67%, respectively, higher Tv-1 (35.49%) fluopicolide-propamocarb (47.82%). According morphological molecular characterization, fungal identified as koningiopsis, T. asperellum, gamsii, respectively. conclusion, exhibited strong oomycete can be integrated into strategies.

Язык: Английский

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Growth and Metabolic Response of Glycine max to the Plant Growth-Promoting Enterobacter Delta PSK and Bradyrhizobium japonicum Under Salinity Stress

Journal of Plant Growth Regulation, Год журнала: 2023, Номер 42(9), С. 5816 - 5830

Опубликована: Март 17, 2023

Abstract This study was designed to test the ability of some plant growth-promoting bacteria adverse effects salinity on soybean growth. The effect Bacillus MAP3 and Enterobacter Delta PSK, along with Bradyrhizobium japonicum studied at two levels NaCl salinization (50 100 mM). physical growth parameters bacterized (21 days old), particularly plants co-inoculated + were significantly enhanced compared control plants. shoot length, leaf area, root chlorophyll a content increased by 49.58%, 78.58%, 20.19%, 57.35%, respectively, indicating promoting activity this bacterial combination. After 19 following onset stress, retarded in controls improved due treatments, especially DeltaPSK, which values all regardless level. Additionally, electrolyte leakage, amounts malondialdehyde hydrogen peroxide decreased considerably combined treatment . Overall, combination PSK original symbiont B. under salt mitigate osmotic stress. strain yield should be further evaluated pave way for its use as biofertilizer japonicum,

Язык: Английский

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Induction of Systemic Resistance to Tobacco mosaic virus in Tomato through Foliar Application of Bacillus amyloliquefaciens Strain TBorg1 Culture Filtrate

Viruses, Год журнала: 2022, Номер 14(8), С. 1830 - 1830

Опубликована: Авг. 20, 2022

The application of microbe-derived products as natural biocontrol agents to boost systemic disease resistance virus infections in plants is a prospective strategy make agriculture more sustainable and environmentally friendly. In the current study, rhizobacterium Bacillus amyloliquefaciens strain TBorg1 was identified based on 16S rRNA, rpoB, gyrA gene sequences, evaluated for its efficiency conferring protection tomato from infection by Tobacco mosaic (TMV). Under greenhouse circumstances, foliar sprays culture filtrate (TBorg1-CF) promoted growth, lowered severity, significantly decreased TMV accumulation systemically infected leaves treated relative untreated controls. reduced 90% following dual treatment, applied 24 h before after infection. Significant increases levels total soluble carbohydrates, proteins, ascorbic acid were also found. addition, significant rise activities enzymes capable scavenging reactive oxygen species (PPO POX), well non-enzymatic oxidative stress markers (H2O2 MDA) observed, compared plants. Enhanced indicated increased transcript polyphenolic pathway (C4H, HCT, CHI) pathogenesis-related (PR-1 PR-5) genes. Out 15 compounds GC-MS analysis, 1,2-benzenedicarboxylic mono(2-ethylhexyl) ester phenol, 2,4-bis(1,1-dimethylethyl), L-proline, N-valeryl-, heptadecyl present highest concentrations ethyl acetate extract TBorg1-CF. amounts n-hexadecanoic acid, pyrrolo [1,2-a] pyrazine-1,4-dione hexahydro-3-(2-methylpropyl)-, nonane, 5-butyl-, eicosane detected. These may act inducers viral Our findings indicate that newly isolated B. could be potentially useful promoting plant growth possible source combating infections.

Язык: Английский

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Impact of Bacillus subtilis and Pseudomonas fluorescens beneficial bacterial agents on soil-borne diseases, growth, and economics of continuous cropping of flue-cured tobacco

Crop Protection, Год журнала: 2023, Номер 177, С. 106556 - 106556

Опубликована: Дек. 12, 2023

Язык: Английский

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Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by Rhizoctonia solani

Agronomy, Год журнала: 2024, Номер 14(2), С. 351 - 351

Опубликована: Фев. 8, 2024

Potato black scurf, caused by Rhizoctonia solani, is a severe soil-borne disease that affects the quality and production of potatoes worldwide. In our study, we used Paenibacillus polymyxa YF Bacillus amyloliquefaciens HT to determine antagonistic ability R. with particular focus on action P. solani its biocontrol effect potato scurf. fermentation, filtrate assay, 50% B. inhibited growth 85.55% 82.86%, respectively. Microscopic observations showed notable morphological changes mycelial collapse, atrophy, deformation following treatment filtrates. Moreover, cell membrane permeability results increased conductivity in bacteria-treated samples compared control. exhibited stable colonization plants secreted various extracellular enzymes (protease, amylase, cellulase), along synthetic substances growth-promoting effects, such as siderophores Indole-3-acetic acid (IAA). Whether it excised tissue inoculation or potted experiment, negative control highest rank symptoms. pot after treatment, physiological parameters remarkable plant height, root length, stem thickness, dry fresh weight. Compared blank control, activities four resistant significantly group. The upregulation group was 4.04, 0.54, 0.46, 3.10 times, PCR analysis identified genes both bacterial strains coding for antimicrobial lipopeptides, including fenB, ituC, srfAA, which are associated fengycin, iturin, surfactin synthesis. These findings demonstrated has prominent suggesting potential an effective biological agent controlling

Язык: Английский

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Transcriptome profiling reveals the impact of various levels of biochar application on the growth of flue-cured tobacco plants

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Июль 10, 2024

Abstract Background Biochar, a carbon-rich source and natural growth stimulant, is usually produced by the pyrolysis of agricultural biomass. It widely used to enhance plant growth, enzyme activity, crop productivity. However, there are no conclusive studies on how different levels biochar application influence these systems. Methods results The present study elucidated dose-dependent effects physiological performance, dry matter accumulation tobacco plants via field experiments. In addition, transcriptome analysis was performed 60-day-old (early stage) 100-day-old (late leaves determine changes in transcript at molecular level under various (0, 600, 1800 kg/ha). demonstrated that optimum enhances regulates enzymatic promotes biomass plants, while higher doses had adverse effects. Furthermore, revealed total 6561 differentially expressed genes (DEGs) were up- or down-regulated groupwise comparison treatments. KEGG pathways carbon fixation photosynthetic organisms (ko00710), photosynthesis (ko00195), starch sucrose metabolism (ko00500) significantly up-regulated optimal dosage (600 kg/ha) (1800 Conclusion Collectively, indicate an rate could positively affect fixation, which turn increased synthesis starch, thus promoting plants. disturbs crucial source-sink balance organic compounds inhibits

Язык: Английский

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Bacillus Amyloliquefaciens IKMM and Zinc Nanoparticles as Biocontrol Candidate Induce the Systemic Resistance by Producing Antioxidants in Tomato Plants Challenged with Early Blight Pathogen

Deleted Journal, Год журнала: 2023, Номер 76(1), С. 87 - 103

Опубликована: Окт. 25, 2023

Early blight disease of tomato is a devastating causing severe yield losses. The chemical control this has become challenge due to emerging fungicide resistance in Alternaria solani. present study investigates the biocontrol potential Bacillus amyloliquefaciens IKMM strain and zinc nanoparticles (Zn-NPs) against A. vitro results demonstrated that B. displayed strong mycelial growth inhibition (88.76%) solani followed by control. Various concentrations (0.1 ppm, 1 ppm 10 50 100 ppm) Zn-NPs conventional mancozeb showed considerable reduction (82.98 76.55% respectively) at higher concentration (100 with EC50 (4.56; R2 0.945) for (8.8937; 0.966351). In greenhouse experiments, plants treated strain, lower severity (13.33%, 16.35% 25.58% infected (69.24%) ultimately increased biomass accumulation. flavonoid contents leaves were after 72 h as compared whereas, 48 inoculation, total phenol recorded. antioxidants enzymes viz., peroxidase (PO), phenlylalanine ammonialyase (PAL) polyphenol oxidase (PPO) significant increase inoculation induced accumulation reactive oxygen species (ROS) down-regulated defense related genes (PO, PPO PAL) activating mechanism inoculation. Under field experiment, caused both seasons under natural infection conditions. fruit was relatively than plants. herein presented indicate indigenous along could be considered putative approach combat challenging pathogen will assist develop biofungicides Future research should focused on use combination batter management strategies sustainable agricultural production system.

Язык: Английский

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Increasing Application of Multifunctional Bacillus for Biocontrol of Pests and Diseases and Plant Growth Promotion: Lessons from Brazil

Agronomy, Год журнала: 2024, Номер 14(8), С. 1654 - 1654

Опубликована: Июль 27, 2024

The microbial genus Bacillus inhabits a diverse range of environments and is widespread across all global biomes, with significant presence in soil habitats. In agriculture, strains play multifaceted roles, serving as biocontrol agents against pests diseases, promoting plant growth by facilitating nutrient availability enhancing stress tolerance. Through mechanisms such phosphate solubilization, ACC-deaminase activity, synthesis phytohormones siderophores, spp. contribute to health crop productivity, new approach regenerative agriculture. ability solubilize makes essential nutrients more accessible plants, while activity helps plants withstand environmental stresses. Additionally, the can stimulate development, siderophores may facilitate uptake iron plants. As agricultural industry embraces Bacillus-based formulations for pest management enhancement, future research holds promising prospects optimizing their applications harnessing full potential agroecosystems. Continued exploration diversity interactions microbiota will further advance sustainable practices. This review contributes understanding how revolutionize agriculture health, increasing providing effective biological solutions diseases. successful application technologies millions hectares Brazilian demonstrates synergy between need practices use bio-inputs.

Язык: Английский

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Potential biocontrol and plant growth promotion of an endophytic bacteria isolated from Glycyrrhiza uralensis seeds

Egyptian Journal of Biological Pest Control, Год журнала: 2022, Номер 32(1)

Опубликована: Май 16, 2022

Abstract Background Endophytic bacteria have been demonstrated to be one of the most potentially important biocontrol agents for their efficiently improved plant growth and protection host plants from infection by phytopathogens. Results Nine strains endophytic isolated Glycyrrhiza uralensis Fisch. ( G. ) seeds were screened inhibitory effect against 5 fungal phytopathogens using dual culture method. Among which, isolate FT 2 showed highest percentage inhibition all test Detached root assay that significantly reduced disease index rotted area on Angelica sinensis slices caused Fusarium acuminatum . also exhibited multiple promotion traits qualitative analytical Based phenotypic, physiological biochemical characterization genotypic characterization, strain was identified as Stenotrophomonas rhizophila Application Cucumber Wilt, equal chemical fungicide, carbendazim. Moreover, increased height, stem diameter, leaf number fresh weight cucumber seedlings activities defense-related enzymes including phenylalanine ammonia-lyase, polyphenol oxidase peroxidase. In addition, could promote seed germination seedling increase enzyme activities, available nutrient contents bacterial count in rhizosphere soil. Conclusions The results used develop environmentally friendly microbial products improve activity pathogenic growth.

Язык: Английский

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