Applied Microbiology,
Год журнала:
2024,
Номер
4(4), С. 1534 - 1548
Опубликована: Ноя. 27, 2024
The
gut
microbiota
plays
an
essential
role
in
its
host’s
nutrition,
development
and
behavior.
Although
crickets
are
becoming
major
ecosystemic
model
systems
have
important
societal
applications,
such
as
alternative
animal
proteins
or
biocatalysts,
little
is
known
about
their
microbiome
acquisition
how
environmental
factors
shape
this
community.
Therefore,
study,
we
exposed
sand
field
to
soils
with
different
characteristics
microbial
communities
test
the
influence
of
these
on
community
composition.
We
used
16S/18S
rRNA
gene
Illumina
sequencing
analyze
soil
communities,
targeting
three
domains
life,
Archaea,
Bacteria,
Eukaryotes.
Our
results
showed
a
dominance
Mucoromycota
fungi
Bacteroidota
microbiota.
were
unable
retrieve
sufficient
read
numbers
for
Archaea.
Most
taxa
that
identified
can
degrade
soil-derived
complex
organic
matter,
likely
helping
host
digest
food.
had
significant
impact
structure,
supporting
our
assumption
environment
acquisition.
Host
sex
also
community,
possibly
because
female
guts
bigger
mass,
leading
differences
oxygen
concentrations.
Process Safety and Environmental Protection,
Год журнала:
2024,
Номер
187, С. 1 - 25
Опубликована: Апрель 28, 2024
The
ever-growing
human
population
has
forced
people
to
intensify
agriculture
meet
the
demand
for
food
and
feed.
However,
increase
in
global
agricultural
production
also
means
an
waste
generation,
which
is
mostly
improperly
managed,
especially
low-
middle-income
countries
where
adequate
management
infrastructure
limited
inefficient.
Consequently,
organic
become
a
burden
due
its
negative
impact
on
environment
health.
In
this
sense,
insect-based
bioconversion
could
be
economically
viable,
environmentally
friendly,
technically
feasible
approach
management.
Based
review,
wastes
from
agriculture,
industry,
household,
municipalities,
starch
industry
are
considered
as
suitable
substrates
bioconverter
insects.
Commonly
used
insects
include
cricket,
yellow
mealworm,
superworm,
black
soldier
fly,
house
flesh
fly
blow
fly.
Rearing
facilities
must
adapted
provide
optimal
environmental
conditions
growth
development
of
reared
insect
maximize
rate
efficiency.
Bioconverter
their
by-products
can
industrially
processed
into
animal
feed,
fertilizer
biodiesel
or
extracted
isolate
bioactive
compounds.
challenges
associated
with
social
acceptance,
method-,
infrastructure-
technology-related
problems,
availability
substrates,
political
legal
regulations.
summary,
potential
reduce
waste,
create
jobs,
novel
products,
offer
sustainable
source
protein
feed
world's
growing
population.
Abstract
Background
Innovations
to
establish
agricultural
value
chains
utilising
side
streams
and
their
reintegration
into
the
feed
food
supply
are
of
great
importance.
Recyclates
derived
from
biomass
waste
therefore
becoming
increasingly
important
as
sources
nutrients.
The
larvae
black
soldier
fly
(BSF;
Hermetia
illucens
)
demonstrate
considerable
potential
livestock
due
ability
utilise
a
wide
range
organic
substrates.
In
this
study,
BSF
(BSFL)
were
reared
on
four
different
substrates:
chicken
diet
(CD),
high-fibre
Gainesville
(FD),
or
FD
supplemented
either
with
biochar
(FD
+
BCH)
single
superphosphate
SSP)
recyclates
sewage
sludge
processing.
To
validate
hypothesis
that
endogenous
substrate-associated
microbiota
significantly
contribute
substrate
conversion,
profiles
BSFL
gut
frass
analysed
by
16S
rRNA
gene
amplicon
sequencing.
Results
associated
substrates
well
body
composition,
growth
performance
data,
mineral
concentration
larvae.
CD
was
superior
in
terms
larval
growth,
although
it
caused
lower
microbial
alpha
diversity
intestine
compared
FD,
dominance
Morganellaceae
families
Lactobacillales
.
addition
two
products
increased
calcium
content
BSFL,
while
phosphorus
only
SSP.
shifts
indicated
BCH
contributed
regulation
milieu
suppressing
potentially
pathogenic
microbes.
SSP
resulted
an
enrichment
microorganisms
attributed
phosphate-solubilising
properties
such
Pseudomonas
fungal
species,
likely
being
responsible
for
improving
bioavailability
substrate.
Conclusions
results
high
adaptability
its
change
through
specific
way
conditions
created
optimal
nutrient
thus
Biodiversitas Journal of Biological Diversity,
Год журнала:
2024,
Номер
24(12)
Опубликована: Янв. 15, 2024
Abstract.
Nguyen
TN,
Tran
TBN,
HN.
2023.
Genetic
diversity
of
black
soldier
flies
in
Vietnam
based
on
DNA
COI
sequence.
Biodiversitas
24:
6727-6732.
The
Black
Soldier
Fly
(BSF),
Hermetia
illucens
(Linnaeus,
1758),
is
found
temperate
and
tropical
regions
the
world.
In
recent
years,
BSF
has
been
cultivated
as
fodder
some
areas
Vietnam.
genetic
not
yet
assessed.
This
study
aimed
to
investigate
nucleotide
sequences
We
examined
22
BSFs
using
or
larval
tissue
samples
for
extraction
subsequent
sequencing.
Our
analysis
showed
that
composition
consisted
25.36%
A,
36.04%
T,
21.25%
C,
17.35%
G,
with
a
significant
prevalence
A+T
nucleotides
61.40%
compared
38.60%
G+C
nucleotides.
Among
individual
collected
from
ten
provinces
cities,
spanning
seven
agro-ecological
Vietnam,
we
identified
9
different
haplotypes,
which
were
distributed
five
clades
but
mainly
clustered
two
main
clades:
E
F.
Clade
E,
prominently
represented
by
branch
higher
abundance
Asia,
while
F
Europe,
Uganda,
South
Korea,
Oceania
North
America.
results
highlight
relatively
high
Vietnamese
population.
addition,
these
similar
those
Asian
Australian
countries,
showing
similarities
Palearctic
Oriental
regions.
These
provide
valuable
insights
inform
breeding
development
The
gut
microbiota
of
black
soldier
fly
larvae
(BSFL,
Hermetia
illucens)
play
a
crucial
role
in
recycling
various
organic
waste
streams.
This
capability
is
linked
to
the
presence
potential
common
core
BSFL.
However,
subjective
thresholds
for
defining
taxa
and
difficulty
separating
genetic
environmental
influences
have
prevented
clear
consensus
literature.
We
analysed
bacterial
communities
two
genetically
distinct
BSF
lines
(wild
type
(WT)
lab-adapted
line
(LD))
raised
on
ten
different
diets
based
agricultural
by-products
food
Southeast
Asia.
Abstract
Background
The
black
soldier
fly
(BSF,
Hermetia
illucens
L.)
is
one
of
the
most
promising
insects
for
bioconversion
organic
waste,
which
often
carry
a
high
microbial
load
with
potential
foodborne
pathogens.
Although
horizontal
transmission
(from
rearing
substrate
to
larvae)
has
been
extensively
studied,
less
known
about
vertical
microorganisms,
and
particularly
pathogens,
across
different
BSF
life
stages.
Results
This
study
investigated
dynamics
Escherichia
coli
stages
(larvae,
prepupae,
pupae
adults)
cycle
its
associated
(chicken
feed)
frass,
based
on
combination
general
counts
(based
culture-dependent
techniques)
bacterial
community
composition
16S
rRNA
gene
sequencing).
Multiple
interactions
between
microbiota
substrate,
frass
larvae
were
affirmed.
showed
relative
consistency
among
both
composition.
Diversification
communities
started
during
pupal
stage,
while
notable
changes
compositions
occurred
metamorphosis
adults.
Furthermore,
E.
was
after
inoculation
approximately
7.0
log
cfu/g
kanamycin-resistant
,
monitoring
from
larval
adult
stage.
still
contained
substantial
levels
(>
4.5
cfu/g)
taken
up
by
larvae,
limited
observed
decreasing
trend
until
prepupal
below
detection
limit
(1.0
all
samples
end
stage
Additionally,
did
not
have
impact
or
Conclusions
fluctuating
underscored
dynamic
character
throughout
.
Hence,
these
findings
paved
way
future
case
studies
pathogens
consecutive
other
insect
species.
