Genetically engineered crops for sustainably enhanced food production systems

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Ноя. 8, 2022

Genetic modification of crops has substantially focused on improving traits for desirable outcomes. It resulted in the development with enhanced yields, quality, and tolerance to biotic abiotic stresses. With advent introducing favorable into crops, biotechnology created a path involvement genetically modified (GM) sustainable food production systems. Although these plants heralded new era crop production, their widespread adoption faces diverse challenges due concerns about environment, human health, moral issues. Mitigating scientific investigations is vital. Hence, purpose present review discuss deployment GM effects provides comprehensive overview cultivation issues preventing adoption, appropriate strategies overcome them. This also presents recent tools genome editing, special focus CRISPR/Cas9 platform. An outline role developed through CRSIPR/Cas9 achieving goals (SDGs) by 2030 discussed detail. Some perspectives approval are laid out age sustainability. The advancement molecular plant editing addresses many facilitates without incorporating transgenic modifications. will allow higher acceptance rate agriculture rapid commercialization. current genetic forecasts increase productivity prosperity agricultural practices. right use potential offer more benefit than harm, its ability alleviate crises around world.

Язык: Английский

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Gene therapy for CNS disorders: modalities, delivery and translational challenges

Nature reviews. Neuroscience, Год журнала: 2024, Номер 25(8), С. 553 - 572

Опубликована: Июнь 19, 2024

Язык: Английский

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A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises

Current Issues in Molecular Biology, Год журнала: 2021, Номер 43(3), С. 1950 - 1976

Опубликована: Ноя. 11, 2021

Genome editing (GE) has revolutionized the biological sciences by creating a novel approach for manipulating genomes of living organisms. Many tools have been developed in recent years to enable complex genomes. Therefore, reliable and rapid increasing yield tolerance various environmental stresses is necessary sustain agricultural crop production global food security. This critical review elaborates GE used improvement. These include mega-nucleases (MNs), such as zinc-finger nucleases (ZFNs), transcriptional activator-like effector (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, this addresses latest advancements role CRISPR/Cas9 genome manipulation major improvement, including quality development biotic stress- abiotic stress-tolerant crops. Implementation technique will lead non-transgene crops with preferred characteristics that can result enhanced capacity under stresses. The be combined current potential breeding methods (e.g., speed omics-assisted breeding) enhance productivity ensure We also discussed challenges limitations CRISPR/Cas9. information useful plant breeders researchers thorough investigation use boost targeting gene interest.

Язык: Английский

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Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 12053 - 12053

Опубликована: Окт. 10, 2022

The major challenges that agriculture is facing in the twenty-first century are increasing droughts, water scarcity, flooding, poorer soils, and extreme temperatures due to climate change. However, most crops not tolerant climatic environments. aim near future, a world with hunger an population, breed and/or engineer tolerate abiotic stress higher yield. Some crop varieties display certain degree of tolerance, which has been exploited by plant breeders develop thrive under conditions. Moreover, long list genes involved tolerance have identified characterized molecular techniques overexpressed individually transformation experiments. Nevertheless, phenotypes polygenetic traits, current genomic tools dissecting exploit their use accelerating genetic introgression using markers or site-directed mutagenesis such as CRISPR-Cas9. In this review, we describe mechanisms sense adverse conditions examine discuss classic new select improve crops.

Язык: Английский

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Targeted introduction of heritable point mutations into the plant mitochondrial genome

Nature Plants, Год журнала: 2022, Номер 8(3), С. 245 - 256

Опубликована: Март 17, 2022

The development of technologies for the genetic manipulation mitochondrial genomes remains a major challenge. Here we report method targeted introduction mutations into plant DNA (mtDNA) that refer to as transcription activator-like effector nuclease (TALEN) gene-drive mutagenesis (GDM), or TALEN-GDM. combines TALEN-induced site-specific cleavage mtDNA with selection confer resistance TALEN cut. Applying TALEN-GDM tobacco nad9 gene, isolated large set mutants carrying single amino acid substitutions in Nad9 protein. could be purified homochondriomy and stably inherited their edited expected maternal fashion. induces both transitions transversions, can access most nucleotide positions within binding site. Our work provides an efficient genome editing produces genetically stable, homochondriomic fertile plants specific point mtDNA.

Язык: Английский

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Gene editing therapeutics based on mRNA delivery

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 200, С. 115026 - 115026

Опубликована: Июль 28, 2023

Язык: Английский

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Breakthrough in CRISPR/Cas system: Current and future directions and challenges

Biotechnology Journal, Год журнала: 2023, Номер 18(8)

Опубликована: Май 11, 2023

Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists develop desirable varieties. This involves inducing site-specific DNA perturbations that can be repaired through repair pathways. GE products currently include CRISPR-associated nuclease breaks, prime editors generated flaps, single nucleotide-modifications, transposases, recombinases. The discovery of double-strand nucleases (SSNs), mechanisms paved the way for targeted GE, first-generation tools, ZFNs TALENs, were successfully utilized GE. However, CRISPR-Cas now become preferred tool due its speed, reliability, cost-effectiveness. Plant functional genomics benefited significantly from widespread use CRISPR advancements developments. review highlights progress made technology, including multiplex editing, base (BE), (PE), as well challenges potential delivery mechanisms.

Язык: Английский

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The progress of molecules and strategies for the treatment of HBV infection

Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13

Опубликована: Март 15, 2023

Hepatitis B virus infections have always been associated with high levels of mortality. In 2019, hepatitis (HBV)-related diseases resulted in approximately 555,000 deaths globally. view its lethality, the treatment HBV has presented a huge challenge. The World Health Organization (WHO) came up ambitious targets for elimination as major public health threat by 2030. To accomplish this goal, one WHO’s strategies is to develop curative treatments infections. Current clinical setting included 1 year pegylated interferon alpha (PEG-IFNα) and long-term nucleoside analogues (NAs). Although both demonstrated outstanding antiviral effects, it difficult cure HBV. reason that covalently closed circular DNA (cccDNA), integrated DNA, viral burden, impaired host immune responses all hinder development overcome these problems, there are trials on number molecules being carried out, -showing promising results so far. review, we summarize functions mechanisms action various synthetic molecules, natural products, traditional Chinese herbal medicines, clustered regularly interspaced short palindromic repeats their proteins (CRISPR/Cas)-based systems, zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs), which could destroy stability life cycle. addition, discuss modulators, can enhance or activate system, well some representative products anti-HBV effects.

Язык: Английский

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Deciphering the recent trends in pesticide bioremediation using genome editing and multi-omics approaches: a review

World Journal of Microbiology and Biotechnology, Год журнала: 2023, Номер 39(6)

Опубликована: Апрель 8, 2023

Язык: Английский

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The paths toward non-viral CAR-T cell manufacturing: A comprehensive review of state-of-the-art methods

Life Sciences, Год журнала: 2024, Номер 348, С. 122683 - 122683

Опубликована: Май 1, 2024

Язык: Английский

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