Integrative Analysis of Transcriptomic Data Reveals a Predictive Gene Signature for Chemoradiotherapy Response in Rectal Cancer
Опубликована: Янв. 1, 2025
BackgroundLocally
advanced
rectal
cancer
(LARC)
is
treated
with
neoadjuvant
chemoradiotherapy
(nCRT)
followed
by
surgery,
achieving
pathological
complete
response
(pCR)
in
~15%
of
cases.
Enhancing
pCR
through
combined
nCRT
and
chemotherapy
increases
toxicity,
while
many
patients
fail
to
benefit
from
nCRT,
experiencing
significant
side
effects.
Predicting
could
enable
non-surgical
management
via
a
watch-and-wait
strategy
spare
non-responders
unnecessary
toxicity.
However,
no
gene
expression
signatures
predicting
have
yet
been
clinically
implemented.MethodsWe
conducted
differential
analysis
across
six
GEO
datasets
integrated
four
microarray
refine
findings.
Using
machine
learning,
we
developed
signature
predictive
validated
it
cross-validation.
Gene
set
enrichment
(GSEA)
identified
biological
pathways
linked
response.
Spatial
transcriptomic
profiling
using
GeoMx
Digital
Profiling
(DSP)
technology
was
performed
on
pre-treatment
biopsies
uncover
compartment-specific
markers
within
tumor
stroma.ResultsThe
achieved
strong
performance
(AUC
=
0.80)
GLMnet
Random
Forest
models
significantly
associated
consensus
molecular
subtypes
(CMS4)
immune
(iCMS3).
GSEA
revealed
related
VEGFR,
EGFR,
Toll-like
receptor
signaling.
transcriptomics
eight
genes,
tumor-associated
genes
showing
higher
value
than
stroma-associated
markers.ConclusionWe
present
novel
LARC
potential
guide
personalized
treatment.
highlights
the
microenvironment's
contribution
therapy
outcomes,
warranting
further
validation
larger
cohorts.
Язык: Английский
Alternative Splicing at the Crossroad of Inflammatory Bowel Diseases and Colitis-Associated Colon Cancer
Cancers,
Год журнала:
2025,
Номер
17(2), С. 219 - 219
Опубликована: Янв. 11, 2025
The
risk
of
developing
colorectal
cancer
(CRC)
is
increased
in
ulcerative
colitis
patients
compared
to
the
general
population.
This
results
from
state
chronic
inflammation,
a
well-known
tumour-promoting
condition.
review
explores
pathologic
and
molecular
characteristics
colitis-associated
colon
(CAC),
emphasizing
distinct
features
sporadic
CRC.
We
focus
on
key
signalling
pathways
involved
transition
CAC,
highlighting
emerging
role
alternative
splicing
these
processes,
namely
how
inflammation-induced
can
significantly
contribute
CRC
observed
among
UC
patients.
calls
for
more
transcriptomic
studies
elucidate
mechanisms
through
which
drives
CAC
pathogenesis.
A
better
understanding
events
crucial
as
they
may
reveal
novel
biomarkers
disease
progression
have
potential
target
changes
therapeutic
strategy.
Язык: Английский
Colorectal Cancer: Current and Future Therapeutic Approaches and Related Technologies Addressing Multidrug Against Multiple Level Resistance Mechanisms
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1313 - 1313
Опубликована: Фев. 4, 2025
Colorectal
cancer
(CRC)
is
the
third
most
common
and
associated
with
a
poor
prognosis.
The
mutation
profile
related
involved
pathways
of
CRC
have
been,
in
broad
terms,
analyzed.
main
current
therapeutic
approaches
been
comprehensively
reviewed
here,
future
possible
therapeu-tic
options
technologies
perspectively
presented.
complex
scenario
represented
by
multiple-level
resistance
mechanism
epidermal
growth
factor
receptor
(EGFR)
pathway,
including
mutations
KRAS,
NRAS,
BRAF
V600E,
discussed.
Examples
engineered
from
literature
along
drug
combination
tested
clinical
trials
are
encouraging
results
observed
latter
(the
BEACON
trial),
totally
free
chemotherapy,
prompted
authors
to
imagine
nanotechnology-assisted
approach
for
bypassing
mechanisms,
hopefully
allowing,
principle,
complete
biological
remission.
Язык: Английский
Identification of SRC, AKT1 and MAPK3 as Therapeutic Targets of Apigenin and Luteolin in Colorectal and Colon Carcinoma through Network Pharmacology
Food Bioscience,
Год журнала:
2025,
Номер
unknown, С. 106313 - 106313
Опубликована: Март 1, 2025
Язык: Английский
Eudragit S 100 Assisted Molecular Solid Dispersion of Andrographolide Tendered Augmented Drug Delivery and Apoptosis in Human Colon Cancer, HT-29 Cells
AAPS PharmSciTech,
Год журнала:
2025,
Номер
26(3)
Опубликована: Март 11, 2025
Язык: Английский
Pan-cancer analysis identifies venous thromboembolism-related genes F3, PLAT, and C1S as potential prognostic biomarkers for glioblastoma and lower grade glioma
Molecular Biomedicine,
Год журнала:
2024,
Номер
5(1)
Опубликована: Авг. 24, 2024
Abstract
Venous
thromboembolism
(VTE)
is
a
prevalent
complication
among
patients
with
cancer,
contributing
significantly
to
morbidity
and
mortality.
However,
the
relationship
between
VTE-related
genes
(VRGs)
their
potential
impact
on
prognosis,
immune
response,
therapeutic
targets
in
various
cancer
types
remains
unclear.
Based
coagulation
complement
pathways,
we
identified
hub
VRGs
that
play
role
regulating
response
cancer.
Specifically,
factor
III
(F3),
plasminogen
activator
(PLAT)
C1s
(C1S)
were
as
exhibit
high
expression
levels,
positively
correlating
tumor
stemness
copy
number
variations,
while
inversely
methylation
particular
types.
Pan-cancer
survival
analysis
revealed
detrimental
effects
of
these
several
types,
notably
glioblastoma
lower
grade
glioma
(GMBLGG).
Further
using
receiver
operating
characteristic
(ROC)
curves
demonstrated
accuracy
F3,
PLAT
C1S
predicting
outcomes
GBMLGG,
area
under
curve
(AUC)
values
ranging
from
0.78
0.9.
Validation
prognostic
value
three
GMBLGG
was
conducted
an
independent
Gene
Expression
Omnibus
(GEO)
dataset.
Additionally,
gene–drug
association
ciclosporin,
ouabain
6-
mercaptopurine,
which
all
immunosuppressive
properties,
options
for
exhibiting
or
expression,
respectively.
In
summary,
our
findings
provide
bioinformatics
perspective
pan-cancer,
highlighting
pivotal
roles
C1S,
could
potentially
be
therapeutically
exploited
targeted
cancers,
especially
GBMLGG.
Язык: Английский