VqERF1B‐VqERF062‐VqNSTS2 transcriptional cascade enhances stilbene biosynthesis and resistance to powdery mildew in grapevine DOI Creative Commons
Chaohui Yan,

Wandi Liu,

Ruimin Li

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Grapes, as one of the world's oldest economic crops, are severely affected by grape powdery mildew, causing significant losses. As a phytoalexin against stilbenes and their key synthetic gene, stilbene synthase (STS), highly sought after researchers. In our previous research, new VqNSTS2, was identified from Vitis quinquangularis accession 'Danfeng-2' through transcriptomic analysis. However, function molecular mechanism VqNSTS2 gene remain unknown. Here, characterization transient overexpression we demonstrated that its expression product, stilbenes, can be detected in model plant tobacco, which does not inherently contain STSs. After artificially inoculating transgenic Arabidopsis lines overexpressing with Erysiphe necator, it found actively moved to pathogen's haustorium responding pathogen, recognized enveloped haustorium, blocking infection invasion exhibited disease resistance. Furthermore, Agrobacterium-mediated stable promoted accumulation enhanced resistance V. vinifera susceptible cultivar 'Thompson Seedless' E. necator. Additionally, screening identification, transcription factor, VqERF062, directly bind DRE RAA motifs on ProVqNSTS2, positively regulating expression. Moreover, VqERF062 interacted VqERF1B promote addition forming homodimer itself. Taken together, findings reveal VqERF1B-VqERF062- module is required for necator providing insights into regulatory biosynthesis.

Язык: Английский

A Comparative Transcriptomic Study Reveals Temporal and Genotype-Specific Defense Responses to Botrytis cinerea in Grapevine DOI Creative Commons
Flavia Angela Maria Maggiolini, Annalisa Prencipe, Carlo Bergamini

и другие.

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 124 - 124

Опубликована: Фев. 7, 2025

Grapevine (Vitis vinifera L.), a globally significant crop, is highly susceptible to Botrytis cinerea, the causative agent of gray mold disease. This study investigates transcriptomic responses B. cinerea in tolerant and grapevine genotypes using RNA sequencing (RNA-seq). Differentially expressed genes (DEGs) were identified at three time points (T1, T2, T3), highlighting both genotype-independent genotype-specific responses. Early-stage infection (T1) revealed rapid robust activation defense pathways genotypes, though genotype showed enhanced modulation metabolic processes by prioritizing secondary metabolism stress adaptation over growth. In contrast, exhibited less coordinated reprogramming, with delayed or weaker key mechanisms. Gene Ontology KEGG analyses critical pathways, including phenylpropanoid biosynthesis-like lignin metabolism, MAPK signaling, as well candidate such WRKY transcription factors enzymes involved cell wall fortification antifungal compound biosynthesis. Genotype-specific emphasized flexibility determinant resistance, exhibiting superior resource allocation pathways. These findings provide insights into molecular basis resistance offering potential targets for breeding genetic engineering enhance resilience reduce fungicide dependency.

Язык: Английский

Процитировано

1
VqERF1B‐VqERF062‐VqNSTS2 transcriptional cascade enhances stilbene biosynthesis and resistance to powdery mildew in grapevine DOI Creative Commons
Chaohui Yan,

Wandi Liu,

Ruimin Li

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Grapes, as one of the world's oldest economic crops, are severely affected by grape powdery mildew, causing significant losses. As a phytoalexin against stilbenes and their key synthetic gene, stilbene synthase (STS), highly sought after researchers. In our previous research, new VqNSTS2, was identified from Vitis quinquangularis accession 'Danfeng-2' through transcriptomic analysis. However, function molecular mechanism VqNSTS2 gene remain unknown. Here, characterization transient overexpression we demonstrated that its expression product, stilbenes, can be detected in model plant tobacco, which does not inherently contain STSs. After artificially inoculating transgenic Arabidopsis lines overexpressing with Erysiphe necator, it found actively moved to pathogen's haustorium responding pathogen, recognized enveloped haustorium, blocking infection invasion exhibited disease resistance. Furthermore, Agrobacterium-mediated stable promoted accumulation enhanced resistance V. vinifera susceptible cultivar 'Thompson Seedless' E. necator. Additionally, screening identification, transcription factor, VqERF062, directly bind DRE RAA motifs on ProVqNSTS2, positively regulating expression. Moreover, VqERF062 interacted VqERF1B promote addition forming homodimer itself. Taken together, findings reveal VqERF1B-VqERF062- module is required for necator providing insights into regulatory biosynthesis.

Язык: Английский

Процитировано

0