In Vitro Evaluation of Silver-Zinc Oxide-Eugenol Nanocomposite for Enhanced Antimicrobial and Wound Healing Applications in Diabetic Conditions DOI Creative Commons
Shunmugiah Karutha Pandian

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed enhance activity promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability chemical composition. Ag+ZnO+EU demonstrated potent efficacy against range associated pathogens, including standard clinical isolates Staphylococcus aureus, Pseudomonas aeruginosaand Candida albicans. Minimum inhibitory concentrations for were significantly lower than those individual components, highlighting synergistic effect nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, effectively eliminated persister-like cells, are typically resistant conventional treatments, suggesting potential solution persistent infections. In vitro scratch human keratinocyte cells that accelerated closure, near-complete observed 24 hours, indicating enhanced cell migration tissue regeneration. Additionally, showed antidiabetic effects increasing glucose uptake up 97.21% in an in assay 2- [N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG), fluorescent analog, applications beyond These findings highlight as promising candidate addressing both resistance impaired diabetic contexts.

Язык: Английский

In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions DOI Creative Commons

Hari Prasath Nagaiah,

Malik Basha Samsudeen,

Akshaya Rani Augustus

и другие.

Discover Nano, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 23, 2025

Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed enhance activity promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability chemical composition. Ag+ZnO+EU demonstrated potent efficacy against range associated pathogens, including standard clinical isolates Staphylococcus aureus, Pseudomonas aeruginosa Candida albicans. Minimum inhibitory concentrations for were significantly lower than those individual components, highlighting synergistic effect nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, effectively eliminated persister-like cells, are typically resistant conventional treatments, suggesting potential solution persistent infections. In vitro scratch human keratinocyte cells that accelerated closure, near-complete observed 24-h, indicating enhanced cell migration tissue regeneration. Additionally, showed antidiabetic effects increasing glucose uptake up 97.21% in an assay 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose, fluorescent analog, applications beyond These findings highlight as promising candidate addressing both resistance impaired diabetic contexts.

Язык: Английский

Процитировано

0
In Vitro Evaluation of Silver-Zinc Oxide-Eugenol Nanocomposite for Enhanced Antimicrobial and Wound Healing Applications in Diabetic Conditions DOI Creative Commons
Shunmugiah Karutha Pandian

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 8, 2024

Abstract Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed enhance activity promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability chemical composition. Ag+ZnO+EU demonstrated potent efficacy against range associated pathogens, including standard clinical isolates Staphylococcus aureus, Pseudomonas aeruginosaand Candida albicans. Minimum inhibitory concentrations for were significantly lower than those individual components, highlighting synergistic effect nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, effectively eliminated persister-like cells, are typically resistant conventional treatments, suggesting potential solution persistent infections. In vitro scratch human keratinocyte cells that accelerated closure, near-complete observed 24 hours, indicating enhanced cell migration tissue regeneration. Additionally, showed antidiabetic effects increasing glucose uptake up 97.21% in an in assay 2- [N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG), fluorescent analog, applications beyond These findings highlight as promising candidate addressing both resistance impaired diabetic contexts.

Язык: Английский

Процитировано

0