Synthesis and Characterization of PEGylated Liposomes and Nanostructured Lipid Carriers with Entrapped Bioactive Triterpenoids: Comparative Fingerprints and Quantification by UHPLC-QTOF-ESI+-MS, ATR-FTIR Spectroscopy, and HPLC-DAD DOI Creative Commons
Carmen Socaciu,

Florinela Fetea,

Mihai Socaciu

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 18(1), С. 33 - 33

Опубликована: Дек. 31, 2024

Background/Objectives: Pentacyclic triterpenoids, as bioactive phytochemicals, have proven to exhibit significant bioactivity (antioxidant, anti-inflammatory, hypoglycemic, and anticancer) low cytotoxicity. This study developed convenient methods for extracting characterizing a birch bark extract enriched in pentacyclic triterpenoids (betulin, betulinic acid, lupeol) entrapped two bioavailable nanoformulations. The performance of ATR-FTIR spectroscopy cost-effective non-destructive method was evaluated comparatively with accurate HPLC-based methods. Methods: pure betulin or acid were used obtain PEGylated liposomes nano lipid carriers (NLCs). Their size characterized by light scattering diffraction. UV-Vis spectrometry applied preliminary evaluation (1), well UHPLC-QTOF-ESI+-MS structure identification (2), (for semi-quantitative evaluation) (3), HPLC-DAD an quantification each component, either the organic solvents nanoformulations (4). Results: had smaller sizes, higher entrapment efficiency, significantly correlated between three analytical positively data confirmed potential this cheaper reliable evaluate efficiency TTs liposome NLC Conclusions: results recommend using liposomal terpenoids their characterization after validation HPLC-DAD. also offers possibility screening short time different recipes stability bioavailability, which is useful investigations vitro vivo, may confirm efficacy therapeutic agents.

Язык: Английский

Quercetin loaded biogenic squalene nano-lipid carriers for the treatment of dry eye syndrome DOI
Ajit Mishra, Jitu Halder,

Ivy Saha

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер 674, С. 125457 - 125457

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

1

Lipid Nanoparticles and Skin: Discoveries and Advances DOI Creative Commons
Debora Santonocito, Carmelo Puglia

Cosmetics, Год журнала: 2025, Номер 12(1), С. 22 - 22

Опубликована: Янв. 27, 2025

Nowadays, skin is one of the organs most commonly affected by diseases (infections, inflammations, and injuries) due to exposure external environment. Although topical treatment represents suitable administration route, it poorly effective low permeability drug through skin. Skin delivery lipid nanocarriers (LNs) appears be a therapeutic strategy overcome these issues, allowing reach or systemic effect. Several LN-based products have been developed enhance permeation bioactive compounds skin, obtaining interesting results in both pharmaceutical cosmetic fields. Therefore, this review aims analyze scientific literature regarding use LNs treat major (psoriasis, wound healing, atopic dermatitis, acne) esthetic defects (wrinkles cellulite). Furthermore, attention has paid transdermal application (topical formulations, patches, microneedles), being new topic recent years.

Язык: Английский

Процитировано

0

Choline-based ionic liquids enhance the dermal delivery of cyclosporine a for potential treatment of psoriasis DOI
Yang Li, Qin Yu, Yi Lü

и другие.

Drug Delivery and Translational Research, Год журнала: 2024, Номер unknown

Опубликована: Сен. 5, 2024

Язык: Английский

Процитировано

3

Liposomal and Nanostructured Lipid Nanoformulations of a Pentacyclic Triterpenoid Birch Bark Extract: Structural Characterization and In Vitro Effects on Melanoma B16-F10 and Walker 256 Tumor Cells Apoptosis DOI Creative Commons
Dumitrița Rugină, Mihai Socaciu, Mǎdǎlina Nistor

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1630 - 1630

Опубликована: Дек. 4, 2024

Background/Objectives: Pentacyclic triterpenoids are increasingly studied as anticancer agents with many advantages compared to synthetic chemotherapeutics. The aim of this study was prepare liposomal and nanostructured lipid formulations including a standardized extract silver birch (Betula pendula) outer bark (TTs) evaluate their potential in vitro, using Melanoma B16-F10 Walker carcinoma cells. Methods: Appropriate solvents were selected for efficient TTs extraction, original recipes used obtain Pegylated liposomes nanolipid complexes entrapped TTs, comparative pure standards (betulinic acid doxorubicin) similar conditions. composition, morphology, sizes all nanoformulations checked by high-performance liquid chromatography/mass spectrometry, Transmission Electronic Microscopy, Diffraction Light Scattering. entrapment efficiency its impact on cell viability, cycle arrest, apoptosis flow cytometry also measured both cancer lines. Conclusions: betulin, lupeol, betulinic acid, showed good stability superior activity acid. According experimental data, NLC nanoformulations, delivery systems natural, biodegradable ingredients enhanced bioavailability. necrosis effects more pronounced types cells, lower cytotoxicity Doxorubicin, can be correlated increased

Язык: Английский

Процитировано

1

Synthesis and Characterization of PEGylated Liposomes and Nanostructured Lipid Carriers with Entrapped Bioactive Triterpenoids: Comparative Fingerprints and Quantification by UHPLC-QTOF-ESI+-MS, ATR-FTIR Spectroscopy, and HPLC-DAD DOI Creative Commons
Carmen Socaciu,

Florinela Fetea,

Mihai Socaciu

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 18(1), С. 33 - 33

Опубликована: Дек. 31, 2024

Background/Objectives: Pentacyclic triterpenoids, as bioactive phytochemicals, have proven to exhibit significant bioactivity (antioxidant, anti-inflammatory, hypoglycemic, and anticancer) low cytotoxicity. This study developed convenient methods for extracting characterizing a birch bark extract enriched in pentacyclic triterpenoids (betulin, betulinic acid, lupeol) entrapped two bioavailable nanoformulations. The performance of ATR-FTIR spectroscopy cost-effective non-destructive method was evaluated comparatively with accurate HPLC-based methods. Methods: pure betulin or acid were used obtain PEGylated liposomes nano lipid carriers (NLCs). Their size characterized by light scattering diffraction. UV-Vis spectrometry applied preliminary evaluation (1), well UHPLC-QTOF-ESI+-MS structure identification (2), (for semi-quantitative evaluation) (3), HPLC-DAD an quantification each component, either the organic solvents nanoformulations (4). Results: had smaller sizes, higher entrapment efficiency, significantly correlated between three analytical positively data confirmed potential this cheaper reliable evaluate efficiency TTs liposome NLC Conclusions: results recommend using liposomal terpenoids their characterization after validation HPLC-DAD. also offers possibility screening short time different recipes stability bioavailability, which is useful investigations vitro vivo, may confirm efficacy therapeutic agents.

Язык: Английский

Процитировано

0