Regenerative Medicine, Год журнала: 2024, Номер unknown, С. 1 - 19
Опубликована: Дек. 17, 2024
Extracellular Vesicles (EVs) became a focus of clinical research when experimental and pre-clinical studies showed that they mimic their parent cells' regenerative therapeutic effects cargo carries disease-specific diagnostic prognostic biomarkers. Since the publication data forms an endpoint study, this review specifically focused on
Язык: Английский
Theranostics, Год журнала: 2024, Номер 14(11), С. 4198 - 4217
Опубликована: Янв. 1, 2024
The utilization of extracellular vesicles (EVs) in wound healing has been well-documented.However, the direct administration free EVs via subcutaneous injection at sites may result rapid dissipation bioactive components and diminished therapeutic efficacy.Functionalized hydrogels provide effective protection, as well ensure sustained release bioactivity during process, making them an ideal candidate material for delivering EVs.In this review, we introduce mechanisms by which accelerate healing, then elaborate on construction strategies engineered EVs.Subsequently, discuss synthesis application delivery systems to enhance complicated healing.Furthermore, face wounds, functionalized with specific microenvironment regulation capabilities, such antimicrobial, anti-inflammatory, immune regulation, used loading EVs, potential approaches addressing these challenges.Ultimately, deliberate future trajectories outlooks, offering a fresh viewpoint advancement artificial intelligence (AI)-energized materials 3D bio-printed multifunctional hydrogel-based dressings biomedical applications.
Язык: Английский
Процитировано
14
Bioactive Materials, Год журнала: 2024, Номер 35, С. 495 - 516
Опубликована: Фев. 21, 2024
During skin aging, the degeneration of epidermal stem cells (EpiSCs) leads to diminished wound healing capabilities and disintegration. This study tackles this issue through a comprehensive analysis combining transcriptomics untargeted metabolomics, revealing age-dependent alterations in
Язык: Английский
Процитировано
11
Journal of Pineal Research, Год журнала: 2024, Номер 76(3)
Опубликована: Апрель 1, 2024
Abstract Osteoporosis (OP) is a severe global health issue that has significant implications for productivity and human lifespan. Gut microbiota dysbiosis been demonstrated to be closely associated with OP progression. Melatonin (MLT) an important endogenous hormone modulates bone metabolism, maintains homeostasis, improves Multiple studies indicated MLT participates in the regulation of intestinal gut barrier function. However, promising effects microbiota‐derived remain unclear. Here, we found resulted tryptophan disorder decreased production MLT, while administration could mitigate OP‐related clinical symptoms reverse dysbiosis, including diversity microbiota, relative abundance many probiotics such as Allobaculum Parasutterella , metabolic function flora amino acid nucleotide energy metabolism. Notably, significantly increased short‐chain fatty acids trimethylamine N‐oxide‐related metabolites. Importantly, modulate dynamic balance M1/M2 macrophages, reduce serum levels pro‐inflammatory cytokines, restore gut‐barrier Taken together, our results highlighted roles microbially derived progression via “gut‐bone” axis SCFA which may provide novel insight into development drug treating OP.
Язык: Английский
Процитировано
10
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101509 - 101509
Опубликована: Янв. 22, 2025
Articular cartilage, composed of chondrocytes within a dynamic viscoelastic matrix, has limited self-repair capacity, posing significant challenge for regeneration. Constructing high-fidelity cartilage organoids through three-dimensional (3D) bioprinting to replicate the structure and physiological functions is crucial regenerative medicine, drug screening, disease modeling. However, commonly used matrix bioinks lack reversible cross-linking precise controllability, hindering cellular regulation. Thus, encoding adaptive cultivating an attractive idea. DNA, with its ability be intricately encoded reversibly cross-linked into hydrogels, offers manipulation at both molecular spatial structural levels. This endows hydrogels viscoelasticity, printability, cell recognition, stimuli responsiveness. paper elaborates on strategies encode bioink via emphasizing regulation predictable properties resulting interactions behavior. The significance these construction highlighted. Finally, we discuss challenges future prospects using DNA-encoded 3D bioprinted organoids, underscoring their potential impact advancing biomedical applications.
Язык: Английский
Процитировано
1
Chinese Medicine, Год журнала: 2025, Номер 20(1)
Опубликована: Фев. 12, 2025
Abstract In the past few years, emergence of organoids and organ-on-a-chip (OOAC) technologies, which are complementary to animal models two-dimensional cell culture methods can better simulate internal environment human body, provides a new platform for traditional Chinese medicine (TCM) studies. Organoids OOAC techniques have been increasingly applied in fields drug screening, assessment development, personalized therapies, developmental biology, there some application cases TCM this review, we summarized current status using organoid technologies research provide key insights future study. It is believed that will play more important roles make greater contributions innovative development TCM.
Язык: Английский
Процитировано
1
Chemical Engineering Journal, Год журнала: 2024, Номер 490, С. 151892 - 151892
Опубликована: Май 3, 2024
Cell-based therapies are used extensively in tissue engineering because of their great biosafety and diverse biological functions. Among them, macrophage lysate provides a comprehensive rich network cytokines to modulate the local immune microenvironment. However, effective strategies for lysate-based biomaterials with immune-regulatory function still lacking. In this study, we engineered silk fibroin hydrogel loaded THP-1 whole-cell regulate microenvironment promote vascularized bone regeneration diabetic defects. Specifically, by modulating expression pattern key enzyme energy metabolism (mitochondrial phosphoenolpyruvate carboxykinase, PCK2) cells, generated enriched anti-inflammatory factors. This facilitated osteogenic differentiation BMSCs angiogenesis HUVECs vitro, incorporating immunomodulatory into enhanced diabetes. Mechanistically, revealed that PCK2 activated nuclear factor-kappa B (NF-κB) signaling pathway macrophages using proteomic profiling. research new insights design cell-derived biomaterials, aiming improve therapeutic outcomes pathological
Язык: Английский
Процитировано
5
Theranostics, Год журнала: 2024, Номер 15(2), С. 682 - 706
Опубликована: Дек. 2, 2024
Organoids, self-organized structures derived from stem cells cultured in a specific three-dimensional (3D) vitro microenvironment, have emerged as innovative platforms that closely mimic vivo cellular behavior, tissue architecture, and organ function. Bone organoids, frontier organoid research, can replicate the complex functional characteristics of bone tissue. Recent advancements led to successful development including models callus, woven bone, cartilage, trabecular marrow. These organoids are widely utilized establishing bone-related disease models, injury repair, drug screening. However, significant discrepancies remain between current human skeletal tissues terms morphology functionality, limiting their ability accurately model physiology pathology. To address these challenges promote standardization construction, evaluation, application we convened experts research teams with substantial expertise field. By integrating existing findings, this consortium aims establish consensus guide future organoids.
Язык: Английский
Процитировано
5
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Янв. 14, 2025
Osteoporosis (OP) is a systemic skeletal disorder characterized by decreased bone mineral density and heightened risk of fractures. Therapies for OP have primarily focused on balancing formation resorption, but enhancing the remineralization osteoporotic also key strategy effective repair. Recent insights into biomineralization mechanisms highlighted essential role mineral-containing extracellular vesicles (EVs) secreted osteoblasts in promoting marrow mesenchymal stromal/stem cell (BMSC) differentiation initiating matrix mineralization. Drawing from these principles, we developed biomimetic approach to replicate structure function osteoblast-derived EVs engineering mitochondrial minerals with homing membranes (CMs). This bone-targeted system exhibits excellent biocompatibility, osteogenic stimulating angiogenesis regulating cellular energy metabolism. Additionally, CM-coated shows affinity collagen fibrils, effectively intrafibrillar mineralization, thereby facilitating Overall, offers safe efficient therapeutic alternative, positioning it as platform tissue regenerative medicine.
Язык: Английский
Процитировано
0
Composites Part B Engineering, Год журнала: 2025, Номер unknown, С. 112255 - 112255
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Current Opinion in Cell Biology, Год журнала: 2025, Номер 94, С. 102519 - 102519
Опубликована: Апрель 22, 2025
Язык: Английский
Процитировано
0