Genome Research,
Год журнала:
2023,
Номер
33(4), С. 572 - 586
Опубликована: Апрель 1, 2023
Epigenetic
modifications
undergo
drastic
erasure
and
reestablishment
after
fertilization.
This
reprogramming
is
required
for
proper
embryonic
development
cell
differentiation.
In
mammals,
some
histone
are
not
completely
reprogrammed
play
critical
roles
in
later
development.
contrast,
nonmammalian
vertebrates,
most
thought
to
be
more
intensively
erased
reestablished
by
the
stage
of
zygotic
genome
activation
(ZGA).
However,
that
escape
vertebrates
their
potential
functional
remain
unknown.
Here,
we
quantitatively
comprehensively
analyzed
modification
dynamics
during
epigenetic
Japanese
killifish,
medaka
(
Oryzias
latipes
)
embryos.
Our
data
revealed
H3K27ac,
H3K27me3,
H3K9me3
complete
reprogramming,
whereas
H3K4
methylation
cleavage
stage.
Furthermore,
experimentally
showed
such
retained
at
early
stages:
(i)
H3K27ac
premarks
promoters
stage,
inhibition
acetyltransferases
disrupts
patterning
H3K27
CpG-dense
promoters,
but
does
affect
chromatin
accessibility
ZGA;
(ii)
globally
specifically
telomeric
regions,
which
maintenance
genomic
stability
These
results
expand
understanding
diversity
conservation
unveil
previously
uncharacterized
functions
reprogramming.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июнь 11, 2024
In
all
eukaryotes,
acetylation
of
histone
lysine
residues
correlates
with
transcription
activation.
Whether
is
a
cause
or
consequence
debated.
One
model
suggests
that
promotes
the
recruitment
and/or
activation
acetyltransferases,
and
occurs
as
ongoing
transcription.
However,
extent
to
which
shapes
global
protein
landscapes
not
known.
Here,
we
show
remains
virtually
unaltered
after
acute
inhibition.
Transcription
inhibition
ablates
co-transcriptionally
occurring
ubiquitylation
H2BK120
but
does
reduce
acetylation.
The
combined
CBP/p300
further
demonstrates
acetyltransferases
remain
active
continue
acetylate
histones
independently
Together,
these
results
mere
transcription;
acetyltransferase
are
uncoupled
from
act
transcription,
non-histone
sustained
in
absence
Intervertebral
disc
degeneration
(IVDD)
is
a
prevalent
cause
of
low
back
pain
and
leading
contributor
to
disability.
IVDD
progression
involves
pathological
shifts
marked
by
low-grade
inflammation,
extracellular
matrix
remodeling,
metabolic
disruptions
characterized
heightened
glycolytic
pathways,
mitochondrial
dysfunction,
cellular
senescence.
Extensive
posttranslational
modifications
proteins
within
nucleus
pulposus
cells
chondrocytes
play
crucial
roles
in
reshaping
the
intervertebral
phenotype
orchestrating
metabolism
inflammation
diverse
contexts.
This
review
focuses
on
pivotal
phosphorylation,
ubiquitination,
acetylation,
glycosylation,
methylation,
lactylation
pathogenesis.
It
integrates
latest
insights
into
various
modification-mediated
inflammatory
signaling
networks,
laying
groundwork
for
targeted
proteomics
metabolomics
treatment.
The
discussion
also
highlights
unexplored
territories,
emphasizing
need
future
research,
particularly
understanding
role
health,
an
area
currently
shrouded
mystery.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Фев. 10, 2022
Abstract
Awakening
of
zygotic
transcription
in
animal
embryos
relies
on
maternal
pioneer
factors.
The
interplay
global
and
specific
functions
these
proteins
remains
poorly
understood.
Here,
we
analyze
chromatin
accessibility
time-resolved
single
double
mutant
zebrafish
lacking
pluripotency
factors
Pou5f3
Sox19b.
We
show
that
two
modify
a
largely
independent
manner.
distinguish
four
types
direct
enhancers
by
differential
requirements
for
or
demonstrate
changes
underlie
the
expression
repertoire
mutants.
Sox19b
promote
linked
to
genes
involved
gastrulation
ventral
fate
specification.
regulating
mesendodermal
dorsal
fates
are
primed
activation
independently
Strikingly,
simultaneous
loss
leads
premature
genes,
regulation
organogenesis
differentiation.
Genome Research,
Год журнала:
2023,
Номер
33(4), С. 572 - 586
Опубликована: Апрель 1, 2023
Epigenetic
modifications
undergo
drastic
erasure
and
reestablishment
after
fertilization.
This
reprogramming
is
required
for
proper
embryonic
development
cell
differentiation.
In
mammals,
some
histone
are
not
completely
reprogrammed
play
critical
roles
in
later
development.
contrast,
nonmammalian
vertebrates,
most
thought
to
be
more
intensively
erased
reestablished
by
the
stage
of
zygotic
genome
activation
(ZGA).
However,
that
escape
vertebrates
their
potential
functional
remain
unknown.
Here,
we
quantitatively
comprehensively
analyzed
modification
dynamics
during
epigenetic
Japanese
killifish,
medaka
(
Oryzias
latipes
)
embryos.
Our
data
revealed
H3K27ac,
H3K27me3,
H3K9me3
complete
reprogramming,
whereas
H3K4
methylation
cleavage
stage.
Furthermore,
experimentally
showed
such
retained
at
early
stages:
(i)
H3K27ac
premarks
promoters
stage,
inhibition
acetyltransferases
disrupts
patterning
H3K27
CpG-dense
promoters,
but
does
affect
chromatin
accessibility
ZGA;
(ii)
globally
specifically
telomeric
regions,
which
maintenance
genomic
stability
These
results
expand
understanding
diversity
conservation
unveil
previously
uncharacterized
functions
reprogramming.
